Abstract
Conductive materials functionalized with redox enzymes provide bioelectronic architectures with application to biological fuel cells and biosensors. Effective electron transfer between the enzyme (biocatalyst) and the conductive materials is imperative for function. Various nanostructured carbon materials are common electrode choices for these applications as both the materials’ inherent conductivity and physical integrity aids optimal performance. The following chapter presents a method for the use of carbon nanotube buckypaper as a conductive architecture suitable for biocatalyst functionalization. In order to securely attach the biocatalyst to the carbon nanotube surface, the conductive buckypaper is modified with the heterobifunctional cross-linker, 1-pyrenebutanoic acid, succinimidyl ester. The technique effectively tethers the enzyme to the carbon nanotube which enhances bioelectrocatalysis, preserves the conductive nature of the carbon surface, and facilities direct electron transfer between the catalyst and material interface. The approach is demonstrated using phenol oxidase (laccase) and pyrroloquinoline quinone-dependent glucose dehydrogenase PQQ-GDH, as representative biocatalysts.
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References
Rincon RA, Lau C, Luckarift HR, Garcia KE, Adkins E, Johnson GR, Atanassov P (2011) Enzymatic fuel cells: integrating flow-through anode and air-breathing cathode into a membrane-less biofuel cell design. Biosens Bioelectron 27:132–136
Ivnitski D, Atanassov P, Apblett C (2007) Direct bioelectrocatalysis of PQQ-dependent glucose dehydrogenase. Electroanalysis 19:1562–1568
Ramasamy RP, Luckarift HR, Ivnitski DM, Atanassov PB, Johnson GR (2010) High electrocatalytic activity of tethered multicopper oxidase-carbon nanotube conjugates. Chem Commun 46:6045–6047
Lau C, Adkins ER, Ramasamy RP, Luckarift HR, Johnson GR, Atanassov P (2011) Design of carbon nanotube-based gas-diffusion cathode for O2 reduction by multicopper oxidases. Adv Energy Mater 2:162–168
Strack G, Luckarift HR, Nichols R, Cozart K, Katz E, Johnson GR (2011) Bioelectrocatalytic generation of directly readable code: harnessing cathodic current for long-term information relay. Chem Commun 47:7662–7664
Narvaez Villarrubia CW, Rincon RA, Radhakrishnan VK, Davis V, Atanassov P (2011) Methylene green electrodeposited on SWNTs-based “bucky” papers for NADH and l-malate oxidation. ACS Appl Mater Interfaces 3:2402–2409
Hussein L, Rubenwolf S, Von Stetten F, Urban G, Zengerle R, Krueger M, Kerzenmacher S (2011) A highly efficient buckypaper-based electrode material for mediatorless laccase-catalyzed dioxygen reduction. Biosens Bioelectron 26:4133–4138
Hussein L, Urban G, Kruger M (2011) Fabrication and characterization of buckypaper-based nanostructured electrodes as a novel material for biofuel cell applications. Phys Chem Chem Phys 13:5831–5839
Ivnitski D, Artyushkova K, Rincon RA, Atanassov P, Luckarift HR, Johnson GR (2008) Entrapment of enzymes and carbon nanotubes in biologically synthesized silica: glucose oxidase-catalyzed direct electron transfer. Small 4:357–364
Vaz-Dominguez C, Campuzano S, Rudiger O, Pita M, Gorbacheva M, Shleev S, Fernandez VM, De Lacey AL (2008) Laccase electrode for direct electrocatalytic reduction of O2 to H2O with high-operational stability and resistance to chloride inhibition. Biosens Bioelectron 24:531–537
Tanne C, Gobel G, Lisdat F (2010) Development of a (PQQ)-GDH-anode based on MWCNT-modified gold and its application in a glucose/O2 biofuel cell. Biosens Bioelectron 26:530–535
Flexer V, Durand F, Tsujimura S, Mano N (2011) Efficient direct electron transfer of PQQ-glucose dehydrogenase on carbon cryogel electrodes at neutral pH. Anal Chem 83:5721–5727
Razumiene J, Vilkanauskyte A, Gureviciene V, Barkauskas J, Meskys R, Laurinavicius V (2006) Direct electron transfer between PQQ dependent glucose dehydrogenases and carbon electrodes: an approach for electrochemical biosensors. Electrochim Acta 51:5150–5156
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Strack, G., Nichols, R., Atanassov, P., Luckarift, H.R., Johnson, G.R. (2013). Modification of Carbon Nanotube Electrodes with 1-Pyrenebutanoic Acid, Succinimidyl Ester for Enhanced Bioelectrocatalysis. In: Guisan, J. (eds) Immobilization of Enzymes and Cells. Methods in Molecular Biology, vol 1051. Humana Press, Totowa, NJ. https://doi.org/10.1007/978-1-62703-550-7_14
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DOI: https://doi.org/10.1007/978-1-62703-550-7_14
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