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Evaluation of Breast Cancer and Melanoma Metastasis in Syngeneic Mouse Models

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Immune Checkpoint Blockade

Part of the book series: Methods in Molecular Biology ((MIMB,volume 1913))

Abstract

Syngeneic mouse models enable us to study the interaction of the immune system with tumor cells during tumor development and metastasis. It further allows us to evaluate the efficacy of immunotherapies on the different stages of tumor growth and the metastatic process. Here we describe two syngeneic mouse models, a murine mammary carcinoma and a murine melanoma model, that are used to study metastasis. Metastases occur spontaneously in the murine mammary carcinoma. The presence and number of foci are evaluated by culturing the lung tissue in a colony formation assay. For the murine melanoma model, tumor cells are injected intravenously, and metastatic burden is analyzed by counting of metastatic lesions.

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Authors and Affiliations

  1. Department of Oncology and Pathology, Karolinska Institutet, Stockholm, Sweden

    Kristina Witt & Andreas Lundqvist

Authors
  1. Kristina Witt
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  2. Andreas Lundqvist
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Corresponding author

Correspondence to Kristina Witt .

Editor information

Editors and Affiliations

  1. Department of Oncology-Pathology, Karolinska Institutet, Stockholm, Sweden

    Yago Pico de Coaña

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Witt, K., Lundqvist, A. (2019). Evaluation of Breast Cancer and Melanoma Metastasis in Syngeneic Mouse Models. In: Pico de Coaña, Y. (eds) Immune Checkpoint Blockade. Methods in Molecular Biology, vol 1913. Humana Press, New York, NY. https://doi.org/10.1007/978-1-4939-8979-9_14

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  • DOI: https://doi.org/10.1007/978-1-4939-8979-9_14

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  • Publisher Name: Humana Press, New York, NY

  • Print ISBN: 978-1-4939-8978-2

  • Online ISBN: 978-1-4939-8979-9

  • eBook Packages: Springer Protocols

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