Abstract
Mesenchymal stem cells (MSCs) are blossoming as a credible source for regenerative medical applications. The use of fetal MSCs is gaining momentum for therapeutic use. The ease of isolation, enhanced characteristics, and immunomodulation properties renders the utilization of fetal MSCs for numerous clinical applications. In this article, we will demonstrate a step-by-step protocol for isolation of Wharton’s jelly MSCs (WJMSCs) from the human umbilical cord matrix, preparation of human platelet lysate, fabricating amniotic membrane scaffold and mice model to study skin regeneration using a combination of MSCs and decellularized amniotic membrane scaffold.
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References
Lazarus GS, Cooper DM, Knighton DR et al (1994) Definitions and guidelines for assessment of wounds and evaluation of healing. Arch Dermatol 130:489–493
Maxson S, Lopez EA, Yoo D et al (2012) Concise review: role of mesenchymal stem cells in wound repair. Stem Cells Transl Med 1:142–149
Sabapathy V, Sundaram B, Vm S et al (2014) Human Wharton’s jelly mesenchymal stem cells plasticity augments scar-free skin wound healing with hair growth. PLoS One 9:e93726
Sabapathy V, Ravi S, Srivastava V et al (2012) Long-term cultured human term placenta-derived mesenchymal stem cells of maternal origin displays plasticity. Stem Cells Int 2012:1–11
Radtke S, Giebel B, Wagner W et al (2014) Platelet lysates and their role in cell therapy. ISBT Sci Ser 9:193–197
Formigli L, Paternostro F, Tani A et al (2015) MSCs seeded on bioengineered scaffolds improve skin wound healing in rats. Wound Repair Regen 23:115–123
Can A, Balci D (2011) Isolation, culture, and characterization of human umbilical cord stroma-derived mesenchymal stem cells. Methods Mol Biol 698:51–62
Woodbury D, Schwarz EJ, Prockop DJ et al (2000) Adult rat and human bone marrow stromal cells differentiate into neurons. J Neurosci Res 61:364–370
Horn P, Bokermann G, Cholewa D et al (2010) Impact of individual platelet lysates on isolation and growth of human mesenchymal stromal cells. Cytotherapy 12:888–898
Wilshaw S-P, Kearney JN, Fisher J et al (2006) Production of an acellular amniotic membrane matrix for use in tissue engineering. Tissue Eng 12:2117–2129
Sabapathy V, Mentam J, Jacob PM et al (2015) Noninvasive optical imaging and in vivo cell tracking of indocyanine green labeled human stem cells transplanted at superficial or in-depth tissue of SCID mice. Stem Cells Int 2015:1–8
Acknowledgments
We are grateful to Department of Biotechnology (DBT), Government of India for Ramalingaswami Fellowship and research support grants (DBT Grants BT/PR8527/MED/31/234/2013; BT/PR8742/AGR/36/773/2013; and BT/PR 15420/MED/31/122/2011) to Sanjay Kumar. We would like to thank University Grants Commission (UGC), New Delhi, India for JRF/SRF fellowship to Balasubramanian Sundaram. We would like to thank Dr. Anne George Cherian, Department of Obstetrics & Gynaecology and Saranya Ravi for valuable assistance during this study.
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Sabapathy, V., Sundaram, B., Kumar, S. (2017). Therapeutic Application of Human Wharton Jelly Mesenchymal Stem Cells in Skin Injury of SCID. In: Di Nardo, P., Dhingra, S., Singla, D. (eds) Adult Stem Cells. Methods in Molecular Biology, vol 1553. Humana Press, New York, NY. https://doi.org/10.1007/978-1-4939-6756-8_9
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DOI: https://doi.org/10.1007/978-1-4939-6756-8_9
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Publisher Name: Humana Press, New York, NY
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Online ISBN: 978-1-4939-6756-8
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