Sister chromatid exchanges are detectable in eukaryotic cells provided with 5-bromo-deoxyuridine for (generally) one cycle of DNA replication (see Fig. S67). Subsequently, at metaphase the chromosomes are stained with either the fluorescent compound Hoechst 33258 (harlequin staining) or according to a special Giemsa procedure.
Sister chromatid exchange. Right: Untreated control. Left: Exposed to the alkylating compound thiotepa during DNA synthesis. (Courtesy of Professor BA Kihlman)
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(2008). Sister Chromatid Exchange (SCE). In: Encyclopedia of Genetics, Genomics, Proteomics and Informatics. Springer, Dordrecht. https://doi.org/10.1007/978-1-4020-6754-9_15702
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DOI: https://doi.org/10.1007/978-1-4020-6754-9_15702
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