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Positron Emission Tomography (PET) Imaging of Metabotropic Glutamate Receptor Subtype 2 (mGlu2) Based on a Negative Allosteric Modulator Radioligand

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Metabotropic Glutamate Receptor Technologies

Part of the book series: Neuromethods ((NM,volume 164))

Abstract

Metabotropic glutamate receptor subtype 2 (mGlu2) is a G-protein-coupled receptor and involved in the physiopathology of several major mental disorders and neurodegenerative diseases. Nowadays, with the development of positron emission tomography (PET) tracers, the distribution and concentration of mGlu2 in vivo can be quantitatively evaluated, which could substantially facilitate disease diagnosis and drug discovery. To demonstrate continuous efforts in the development of mGlu2 PET tracers, we present an example with detailed protocols, including step-by-step procedures of chemical synthesis of an mGlu2-negative allosteric modulator, namely 7-((2,5-dioxopyrrolidin-1-yl)methyl)-4-(2-fluro-4-methoxyphenyl) quinoline-2-carboxamide (QCA), radiolabeling of [11C]QCA, ex vivo whole-body biodistribution, autoradiography, and dynamic PET imaging.

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Author information

Authors and Affiliations

  1. Division of Nuclear Medicine and Molecular Imaging, Massachusetts General Hospital, Boston, MA, USA

    Xiaofei Zhang, Zhiwei Xiao, Lee Josephson, Lu Wang & Steven H. Liang

  2. Department of Radiology, Harvard Medical School, Boston, MA, USA

    Xiaofei Zhang, Zhiwei Xiao, Lee Josephson, Lu Wang & Steven H. Liang

  3. Department of Nuclear Medicine and PET/CT-MRI Center, Center of Cyclotron and PET Radiopharmaceuticals, The First Affiliated Hospital of Jinan University, Guangzhou, China

    Zhiwei Xiao & Lu Wang

  4. Department of Radiopharmaceuticals Development, National Institute of Radiological Sciences, National Institutes for Quantum and Radiological Science and Technology, Chiba, Japan

    Katsushi Kumata, Tomoteru Yamasaki & Ming-Rong Zhang

Authors
  1. Xiaofei Zhang
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  2. Zhiwei Xiao
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  3. Katsushi Kumata
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  4. Tomoteru Yamasaki
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  5. Lee Josephson
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  6. Ming-Rong Zhang
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  7. Lu Wang
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  8. Steven H. Liang
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Corresponding author

Correspondence to Steven H. Liang .

Editor information

Editors and Affiliations

  1. Psychology Department, Arizona State University, Tempe, AZ, USA

    M Foster Olive

  2. Phoenix Children’s Hospital, Barrow Neurological Institute, Phoenix, AZ, USA

    Brian T. Burrows

  3. Psychology Department, Arizona State University, Tempe, AZ, USA

    Jonna M. Leyrer-Jackson

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Zhang, X. et al. (2021). Positron Emission Tomography (PET) Imaging of Metabotropic Glutamate Receptor Subtype 2 (mGlu2) Based on a Negative Allosteric Modulator Radioligand. In: Olive, M.F., Burrows, B.T., Leyrer-Jackson, J.M. (eds) Metabotropic Glutamate Receptor Technologies. Neuromethods, vol 164. Humana, New York, NY. https://doi.org/10.1007/978-1-0716-1107-4_2

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  • DOI: https://doi.org/10.1007/978-1-0716-1107-4_2

  • Published:

  • Publisher Name: Humana, New York, NY

  • Print ISBN: 978-1-0716-1106-7

  • Online ISBN: 978-1-0716-1107-4

  • eBook Packages: Springer Protocols

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