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Chiral separation of ketoprofen on an achiral NH2 column by HPLC using vancomycin as chiral mobile phase additive

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Abstract

A high-performance liquid chromatographic method for chiral separation of ketoprofen racemate was developed. (R)- and (S)-ketoprofen enantiomers were separated on a LiChrosorb NH2 column (250 mm × 4.6 mm, i.d 5 µm) at 20 °C, using 2-propanol/potassium dihydrogen phosphate buffer (pH 6.0, 0.05 M) (50:50 v/v). Containing vancomycin as the mobile phase, at a flow rate of 0.8 ml min−1 and detection wavelength of UV, the detector was set at 310 nm. Under these conditions, ketoprofen enantiomers could be separated with a selectivity factor (α) of 2.172 and a resolution (Rs) of 4.78 using extremely low concentrations of the vancomycin chiral additive.

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Authors and Affiliations

  1. Laboratory to Research of Bioactive Products and Enhancement of Biomass, Department of Chemistry, Ecole Normale Superieur Vieux Kouba, 16050, Algiers, Algeria

    Dehbiya Gherdaoui, Hafsa Bekdouche, Said Zerkout & Rachid Fegas

  2. CNRS, iSm2 UMR 7313, Aix Marseille Université, 13397, Marseille, France

    Dehbiya Gherdaoui, Rachid Fegas & Michel Righezza

Authors
  1. Dehbiya Gherdaoui
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  2. Hafsa Bekdouche
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  3. Said Zerkout
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  4. Rachid Fegas
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  5. Michel Righezza
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Correspondence to Dehbiya Gherdaoui.

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Gherdaoui, D., Bekdouche, H., Zerkout, S. et al. Chiral separation of ketoprofen on an achiral NH2 column by HPLC using vancomycin as chiral mobile phase additive. J IRAN CHEM SOC 13, 2319–2323 (2016). https://doi.org/10.1007/s13738-016-0951-6

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  • DOI: https://doi.org/10.1007/s13738-016-0951-6

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