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Sequence analysis and expression of the calmodulin gene, MCaM-3, in mulberry (Morus L.)

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Abstract

A full-length cDNA sequence encoding CaM from mulberry, which we designated MCaM-3 (GenBank accession No: GQ303247), was cloned based on mulberry expressed sequence tags(ESTs). Sequence analysis showed that MCaM-3 is 951 base pairs in length, encoding 149 amino acids with a predicted molecular weight of 16.85 kD and an isoelectric point of 3.95. Online SMART analysis showed that the MCaM-3 protein has four EFh functional domains, which can bind calcium. The expression level of MCaM-3at different developmental stages in mulberry leaves and flowers and in different tissues was investigated. The results showed that MCaM-3 transcripts are most abundantly expressed in mature tissues, such as mature female flowers and climax leaves, and the expression level of the mRNA could be increased significantly under low temperature, drought, and salt stress conditions compared to the normal growth environment. This research will help us understand the resistance mechanism of functional genes in mulberry.

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References

  • Al-Quraan NA, Locy RD and Singh NK (2010) Expression of calmodulin genes in wild type and calmodulin mutants of Arabidopsis thaliana under heat stress. Plant Physiol. Biochem. 48: 697–702.

    Article  PubMed  CAS  Google Scholar 

  • Aravind P, Chandra K, Reddy PP, Jeromin A, Chary KV and Sharma Y (2008) Regulatory and structural EF-hand motifs of neuronal calcium sensor-1: Mg2+ modulates Ca2+ binding, Ca2+-induced conformational changes, and equilibrium unfolding transitions. J. Mol. Biol. 376: 1100–1115.

    Article  PubMed  CAS  Google Scholar 

  • Camas A, Cardenas L, Quinto C and Lara M (2002) Expression of different calmodulin genes in bean (Phaseolus vulgaris L.): role of nod factor on calmodulin gene regulation. Mol. Plant Microbe Interact. 15: 428–436.

    Article  PubMed  CAS  Google Scholar 

  • Cao Y, Song F, Goodman RM and Zheng Z (2006) Molecular characterization of four rice genes encoding ethylene-responsive transcriptional factors and their expressions in response to biotic and abiotic stress. J. Plant Physiol. 163: 1167–1178.

    Article  PubMed  CAS  Google Scholar 

  • Capozzi F, Casadei F and Luchinat C (2006) EF-hand protein dynamics and evolution of calcium signal transduction: an NMR view. J. Biol. Inorg. Chem. 11: 949–962.

    Article  PubMed  CAS  Google Scholar 

  • Cook SJ and Lockyer PJ. 2006. Recent advances in Ca2+-dependent Ras regulation and cell proliferation. Cell Calcium 39: 101–112.

    Article  PubMed  CAS  Google Scholar 

  • Duplat D, Puissegur M, Bedouet L, Rousseau M, Boulzaguet H, Milet C, Sellos D, Van Wormhoudt A and Lopez E (2006) Identification of calconectin, a calcium-binding protein specifically expressed by the mantle of Pinctada margaritifera. FEBS Lett. 580: 2435–2441.

    Article  PubMed  CAS  Google Scholar 

  • Folzer H, Capelli N, Dat J and Badot PM (2005) Molecular cloning and characterization of calmodulin genes in young oak seedlings (Quercus petraea L.) during early flooding stress. Biochim. Biophys. Acta 1727: 213–219.

    PubMed  CAS  Google Scholar 

  • Galon Y, Nave R, Boyce JM, Nachmias D, Knight MR and Fromm H (2008) Calmodulin-binding transcription activator (CAMTA) 3 mediates biotic defense responses in Arabidopsis. FEBS Lett. 582: 943–948.

    Article  PubMed  CAS  Google Scholar 

  • Gao Y, Gillen CM and Wheatly MG (2009) Cloning and characterization of a calmodulin gene (CaM) in crayfish Procambarus clarkii and expression during molting. Comp. Biochem. Physiol. B: Biochem. Mol. Biol. 152: 216–225.

    Article  Google Scholar 

  • Gifford JL, Walsh MP and Vogel HJ (2007) Structures and metalion-binding properties of the Ca2+-binding helix-loop-helix EF-hand motifs. Biochem. J. 405: 199–221.

    Article  PubMed  CAS  Google Scholar 

  • Gonzalez-Andrade M, Figueroa M, Rodriguez-Sotres R, Mata R and Sosa-Peinado A (2009) An alternative assay to discover potential calmodulin inhibitors using a human fluorophore-labeled CaM protein. Anal. Biochem. 387: 64–70.

    Article  PubMed  CAS  Google Scholar 

  • Grabarek Z (2006) Structural basis for diversity of the EF-hand calcium-binding proteins. J. Mol. Biol. 359: 509–525.

    Article  PubMed  CAS  Google Scholar 

  • Gu Z, Ma B, Jiang Y, Chen Z, Su X and Zhang H (2008) Expression analysis of the calcineurin B-like gene family in rice (Oryza sativa L.) under environmental stresses. Gene 415: 1–12.

    Article  PubMed  CAS  Google Scholar 

  • Gut H, Dominici P, Pilati S, Astegno A, Petoukhov MV, Svergun DI, Grutter MG and Capitani G (2009) A common structural basis for pH- and calmodulin-mediated regulation in plant glutamate decarboxylase. J. Mol. Biol. 392: 334–351.

    Article  PubMed  CAS  Google Scholar 

  • Hamada S, Seiki Y, Watanabe K, Ozeki T, Matsui H and Ito H (2009) Expression and interaction of the CBLs and CIPKs from immature seeds of kidney bean (Phaseolus vulgaris L.). Phytochemistry 70: 501–507.

    Article  PubMed  CAS  Google Scholar 

  • Hong-Bo S, Li-Ye C, Ming-An S, Shi-Qing L and Ji-Cheng Y (2008) Bioengineering plant resistance to abiotic stresses by the global calcium signal system. Biotechnol. Adv. 26: 503–510.

    Article  PubMed  Google Scholar 

  • Koo SC(2009) The calmodulin-binding transcription factor OsCBT suppresses defense responses to pathogens in rice. Mol. Cells 27: 563–570.

    Google Scholar 

  • Lee SM, Kim HS, Han HJ, Moon BC, Kim CY, Harper JF and Chung WS (2007) Identification of a calmodulin-regulated autoinhibited Ca2+-ATPase (ACA11) that is localized to vacuole membranes in Arabidopsis. FEBS Lett. 581: 3943–3949.

    Article  PubMed  CAS  Google Scholar 

  • Li DF, Li J, Ma L, Zhang L and Lu YT (2006) Calmodulin isoform-specific activation of a rice calmodulin-binding kinase conferred by only three amino-acids of OsCaM61. FEBS Lett. 580: 4325–4331.

    Article  PubMed  CAS  Google Scholar 

  • Luan J, Liu Z, Zhang S, Li H, Fan C and Li L (2007) Characterization, evolution and expression of the calmodulin1 genes from the amphioxus Branchiostoma belcheri tsingtauense. Acta Biochim. Biophys. Sin. (Shanghai) 39: 255–264.

    Article  CAS  Google Scholar 

  • Ma YY, Song WY, Liu ZH, Zhang HM, Guo XL, Shao HB and Ni FT (2009) The dynamic changing of Ca2+ cellular localization in maize leaflets under drought stress. C. R. Biol. 332: 351–362.

    Article  PubMed  CAS  Google Scholar 

  • Maghuly F, Khan MA, Fernandez EB, Druart P, Watillon B and Laimer M (2008) Stress regulated expression of the GUS-marker gene (uidA) under the control of plant calmodulin and viral 35S promoters in a model fruit tree rootstock: Prunus incisa x serrula. J. Biotechnol. 135: 105–116.

    Article  PubMed  CAS  Google Scholar 

  • Mahajan S and Tuteja N (2005) Cold, salinity and drought stresses: an overview. Arch Biochem. Biophys. 444: 139–158.

    Article  PubMed  CAS  Google Scholar 

  • Mamrutha HM, Mogili T, Jhansi Lakshmi K, Rama N, Kosma D, Udaya Kumar M, Jenks MA and Nataraja KN (2010) Leaf cuticular wax amount and crystal morphology regulate post-harvest water loss in mulberry (Morus species). Plant Physiol. Biochem. 48: 690–696.

    Article  PubMed  CAS  Google Scholar 

  • McCormack E, Tsai YC and Braam J (2005) Handling calcium signaling: Arabidopsis CaMs and CMLs. Trends Plant Sci. 10: 383–389.

    Article  PubMed  CAS  Google Scholar 

  • Morgan RO, Martin-Almedina S, Garcia M, Jhoncon-Kooyip J and Fernandez MP (2006) Deciphering function and mechanism of calcium-binding proteins from their evolutionary imprints. Biochim. Biophys. Acta 1763: 1238–1249.

    Article  PubMed  CAS  Google Scholar 

  • Orojan I, Bakota L and Gulya K (2006) Differential calmodulin gene expression in the nuclei of the rat midbrain-brain stem region. Acta Histochem. 108: 455–462.

    Article  PubMed  CAS  Google Scholar 

  • Pan G and Lou C (2008) Isolation of an 1-aminocyclopropane-1-carboxylate oxidase gene from mulberry (Morus alba L.) and analysis of the function of this gene in plant development and stresses response. J. Plant Physiol. 165: 1204–1213.

    Article  PubMed  CAS  Google Scholar 

  • Rai MK, Asthana P, Singh SK, Jaiswal VS and Jaiswal U (2009) The encapsulation technology in fruit plants: A review. Biotechnol. Adv. 27: 671–679.

    Article  PubMed  Google Scholar 

  • Roche J, Hewezi T, Bouniols A and Gentzbittel L (2009) Real-time PCR monitoring of signal transduction related genes involved in water stress tolerance mechanism of sunflower. Plant Physiol. Biochem. 47: 139–145.

    Article  PubMed  CAS  Google Scholar 

  • Schaub MC and Heizmann CW (2008) Calcium, troponin, calmodulin, S100 proteins: from myocardial basics to new therapeutic strategies. Biochem. Biophys. Res. Commun. 369: 247–264.

    Article  PubMed  CAS  Google Scholar 

  • Shao HB, Song WY and Chu LY (2008) Advances of calcium signals involved in plant anti-drought. C. R. Biol. 331: 587–596.

    Article  PubMed  CAS  Google Scholar 

  • Vanderbeld B and Snedden WA(2007) Developmental and stimulus-induced expression patterns of Arabidopsis calmodulin-like genes CML37, CML38 and CML39. Plant Mol. Biol. 64: 683–697.

    Article  PubMed  CAS  Google Scholar 

  • Wang JH, Thampatty BP, Lin JS and Im HJ (2007) Mechanoregulation of gene expression in fibroblasts. Gene 391: 1–15.

    Article  PubMed  CAS  Google Scholar 

  • Wang Y, Ma H, Liu G, Zhang D, Ban Q, Zhang G, Xu C and Yang C (2008) Generation and analysis of expressed sequence tags from a NaHCO3-treated Limonium bicolor cDNA library. Plant Physiol. Biochem. 46: 977–986.

    Article  PubMed  CAS  Google Scholar 

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Authors and Affiliations

  1. Sericultural Research Institute, Chinese Academy of Agricultural Sciences, Zhenjiang Jiangsu, 212018, China

    Rongjun Fang, Dongqing Hu, Yinghua Zhang, Long Li, Weiguo Zhao, Li Liu & Jialin Cheng

  2. School of Life Sciences, Nanjing University, Nanjing Jiangsu, 210093, China

    Rongjun Fang, Jinliang Qi & Yonghua Yang

  3. College of biological and Environmental Engineering, Jiangsu University of Science and technology, Zhenjiang Jiangsu, 212018, China

    Dongqing Hu, Yinghua Zhang, Long Li & Weiguo Zhao

Authors
  1. Rongjun Fang
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  2. Dongqing Hu
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  3. Yinghua Zhang
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  4. Long Li
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  5. Weiguo Zhao
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  6. Li Liu
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  7. Jialin Cheng
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  8. Jinliang Qi
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  9. Yonghua Yang
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Correspondence to Long Li or Weiguo Zhao.

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Fang, R., Hu, D., Zhang, Y. et al. Sequence analysis and expression of the calmodulin gene, MCaM-3, in mulberry (Morus L.). Genes Genom 33, 97–103 (2011). https://doi.org/10.1007/s13258-010-0124-4

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  • DOI: https://doi.org/10.1007/s13258-010-0124-4

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