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Antioxidant effect of astragalin isolated from the leaves of Morus alba L. against free radical-induced oxidative hemolysis of human red blood cells

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Abstract

We evaluated the antioxidant properties of mulberry leaves extract (MLE) and flavonoids isolated from MLE. MLE was prepared by extraction with methanol. Flavonoids were analyzed by high-performance liquid chromatography. Oxidative hemolysis of normal human red blood cells (RBCs) was induced by the aqueous peroxyl radical [2,2′-Azobis (2-amidinopropane) dihydrochloride, AAPH]. MLE contained three flavonoids in the order quercetin (QC) > kaempferol (KF) > astragalin (AG). Oxidative hemolysis of RBCs induced by AAPH was suppressed by MLE, AG, KF, and QC in a time- and dose-dependent manner. MLE and these three flavonoids prevented the depletion of cystosolic antioxidant glutathione (GSH) in RBCs. AG had the greatest protective effect against AAPH-induced oxidative hemolysis and GSH depletion in RBCs.

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Acknowledgments

This work was carried out with the support of “Cooperative Research Program for Agriculture Science & Technology Development (Project No. 00966303)” Rural Development Administration, Republic of Korea.

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Authors and Affiliations

  1. Department of Radiological Sciences, Jeonju University, Jeonju, 560-759, Korea

    Jiwon Choi

  2. Department of Healthcare & Science, Jeonju University, Jeonju, 560-759, Korea

    Hyun Ju Kang & Seon Il Jang

  3. Department of Physiology, School of Medicine, Chonbuk National University, Jeonju, 561-756, Korea

    Jiwon Choi & Sung Zoo Kim

  4. College of Life Science and Natural Resources, Wonkwang University, Iksan, 570-749, Korea

    Tae Oh Kwon

  5. Jeonju Biomaterials Institute, Jeonju, 561-360, Korea

    Seung-Il Jeong

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  1. Jiwon Choi
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  2. Hyun Ju Kang
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Correspondence to Seon Il Jang.

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Choi, J., Kang, H.J., Kim, S.Z. et al. Antioxidant effect of astragalin isolated from the leaves of Morus alba L. against free radical-induced oxidative hemolysis of human red blood cells. Arch. Pharm. Res. 36, 912–917 (2013). https://doi.org/10.1007/s12272-013-0090-x

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  • DOI: https://doi.org/10.1007/s12272-013-0090-x

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