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Protective Effect of Hydroxytyrosol Against Cardiac Remodeling After Isoproterenol-Induced Myocardial Infarction in Rat

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Abstract

The present study aimed to investigate the cardioprotective effect of hydroxytyrosol (HT) against isoproterenol-induced myocardial infarction in rats. Male rats were randomly divided into four groups, control, isoproterenol (Isop) and pretreated animals with HT in two different doses (2 and 5 mg/kg) orally for 7 days and intoxicated with isoproterenol (Isop + HT1) and (Isop + HT2) groups. Myocardial infarction in rats was induced subcutaneously by isoproterenol (100 mg/kg, s.c.) at an interval of 24 h on 6th and 7th day. On 8th day, electrocardiographic (ECG) pattern, gravimetric and biochemical parameters were assessed. Isoproterenol exhibited changes in ECG pattern, including significant ST-segment elevation and increase in the serum troponin-T level by 317 % as compared to control rats. Moreover, cardiac injury markers (creatine kinase-MB, lactate dehydrogenase, alanine aminotransferase) underwent a notable rise in serum of infarcted animals. Else, a disturbance in lipids profile and significant increase in lipase and angiotensin-converting enzyme (ACE) activities and heart weight ratio were observed in isoproterenol group. However, pre- and co-treatment with HT (2 and 5 mg/kg) improved the myocardium injury, restored the hemodynamic function and inhibited the ACE activity that prevent cardiac hypertrophy and remodeling. Overall, these findings demonstrated that HT exerted a potent cardioprotective effect against isoproterenol-induced myocardial infarction.

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Acknowledgments

This research was supported by the Tunisian Ministry of Higher Education and Scientific Research and the Tunisian Ministry of Public Health. Authors wish to thank Mrs. Sadok Slema, Mbarek Nasri and Chedly Tmar for their assistance and cooperation.

Conflict of interests

The authors declare no conflict of interest.

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    Authors and Affiliations

    1. Laboratory of Animal Physiology, Faculty of Sciences of Sfax, University of Sfax, P.O. Box 95, 3052, Sfax, Tunisia

      Kais Mnafgui & Abdelfattah Elfeki

    2. Department of Internal Medicine, Hospital of Sidi Bouzid, 9100, Sidi Bouzid, Tunisia

      Kais Mnafgui, Raouf Hajji & Fatma Derbali

    3. Laboratory of Chemistry of Natural Products, Faculty of Sciences of Sfax, University of Sfax, B.P. 1171, 3000, Sfax, Tunisia

      Ines Khlif & Noureddine Allouche

    4. Faculty of Medicine of Monastir, Avenue Avicenne, 5019, Monastir, Tunisia

      Ftouma Kraiem

    5. Department of Cardiology, Centre Hospitalier Intercommunal Robert Ballanger, 93600, Boulevard Robert Ballanger, Aulnay-Sous-Bois, France

      Hedi Ellefi

    6. Laboratory of Plant Biotechnology, Faculty of Sciences of Sfax, University of Sfax, B.P. 1171, 3000, Sfax, Tunisia

      Neji Gharsallah

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    1. Kais Mnafgui
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    Correspondence to Kais Mnafgui.

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    Raouf Hajji and Fatma Derbali have contributed equally to this work.

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    Mnafgui, K., Hajji, R., Derbali, F. et al. Protective Effect of Hydroxytyrosol Against Cardiac Remodeling After Isoproterenol-Induced Myocardial Infarction in Rat. Cardiovasc Toxicol 16, 147–155 (2016). https://doi.org/10.1007/s12012-015-9323-1

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    • DOI: https://doi.org/10.1007/s12012-015-9323-1

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