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Lead Modulates Calcium Entry and Beta-Adrenoceptors Signaling to Produce Myometrial Relaxation in Rats

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Abstract

Modulation of myometrial spontaneity by lead acetate trihydrate (Pb) and its regulatory pathways were studied in estrogenized rats. Isometric tension in myometrial strips under a resting tension of 1 g was measured using data acquisition system-based physiograph and Lab Chart Pro v7.3.7 software. Lead produced a dose-dependent inhibitory effect on rat myometrium with a major effect on phasic contractions compared to tonic contractions along with a reduction in both amplitude and frequency of contraction. Lead (3 μM) significantly (p < 0.05) reduced CaCl2, and 80 mM KDS induced contractile response while potentiated the relaxant effect of phenylephrine. Based on our findings, it may be inferred that lead blocks calcium entry through VDCC and/or stimulates β-adrenoceptors adenylyl cyclase-C-AMP pathway to produce inhibitory effect on rat myometrium.

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Acknowledgment

This research work was supported by the Indian Council of Agricultural Research (ICAR), New Delhi under Niche Area of Excellence Programme to Department of Veterinary Pharmacology and Toxicology, DUVASU, Mathura, India (Grant No.:10(10)/2012-EPD, dated: 23rd March, 2012). Financial assistance from ICAR is thankfully acknowledged.

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Authors and Affiliations

  1. Experimental Pharmacology Laboratory, Department of Veterinary Pharmacology and Toxicology, College of Veterinary Science and Animal Husbandry, U.P. Pandit Deen Dayal Upadhyaya Pashu Chikitsa Vigyan Vishwavidyalaya Evam Go-Anusandhan Sansthan, (DUVASU), Mathura, 281001, India

    Udayraj P. Nakade, Abhishek Sharma, Soumen Choudhury, Rajkumar Singh Yadav & Satish Kumar Garg

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  1. Udayraj P. Nakade
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  2. Abhishek Sharma
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  3. Soumen Choudhury
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  4. Rajkumar Singh Yadav
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  5. Satish Kumar Garg
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Correspondence to Satish Kumar Garg.

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Nakade, U.P., Sharma, A., Choudhury, S. et al. Lead Modulates Calcium Entry and Beta-Adrenoceptors Signaling to Produce Myometrial Relaxation in Rats. Biol Trace Elem Res 176, 176–180 (2017). https://doi.org/10.1007/s12011-016-0813-1

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  • DOI: https://doi.org/10.1007/s12011-016-0813-1

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