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Fargesin, a component of Flos Magnoliae, stimulates glucose uptake in L6 myotubes

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Abstract

Flos Magnoliae (FM) is a commonly used Chinese medicinal herb for symptomatic relief of allergic rhinitis, sinusitis and headache. Although several FM species have been used as substitutes or adulterants for clinical use, possible differences in their pharmacological actions have not been reported. To confirm the effects of FM on skeletal muscle glucose metabolism, we tested the effects of several compounds isolated from FM on glucose uptake by L6 myotubes. We found that fargesin, a component of FM, dose-dependently stimulated glucose consumption in L6 myotubes, which was accompanied by enhanced glucose transporter (GLUT)-4 translocation to the cell surface. Fargesin-stimulated glucose uptake was blocked by wortmannin, a phosphatidylinositol-3 kinase (PI3 K) inhibitor. Fargesin stimulated Akt phosphorylation, a key component in the insulin signaling pathway, which was completely inhibited by wortmannin. Here, we demonstrated that fargesin, a bioactive component of Flos Magnoliae, increases basal glucose uptake and GLUT4 translocation in L6 myotubes by activating the PI3 K–Akt pathway.

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Acknowledgments

This study was performed at a laboratory supported by an endowment from Erina Co., Inc.

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Authors and Affiliations

  1. Research Institute for Biological Functions, Chubu University, 1200 Matsumoto, Kasugai, Aichi, Japan

    Sun-Sil Choi, Byung-Yoon Cha, Bong-Keun Choi, Young-Sil Lee, Takayuki Yonezawa, Kazuo Nagai & Je-Tae Woo

  2. Faculty of Education, University of the Ryukyus, 1 Senbaru, Nishihara, Okinawa, 903-0213, Japan

    Toshiaki Teruya

  3. Department of Biological Chemistry, Chubu University, 1200 Matsumoto, Kasugai, Aichi, Japan

    Toshiaki Teruya

  4. Department of Research and Development, Erina Co., Inc., 1-9-2 Hagashi-Shinbash, Minato-ku, Tokyo, 105-0021, Japan

    Kazuo Nagai & Je-Tae Woo

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  1. Sun-Sil Choi
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  2. Byung-Yoon Cha
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  3. Bong-Keun Choi
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  4. Young-Sil Lee
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  8. Je-Tae Woo
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Correspondence to Byung-Yoon Cha.

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Choi, SS., Cha, BY., Choi, BK. et al. Fargesin, a component of Flos Magnoliae, stimulates glucose uptake in L6 myotubes. J Nat Med 67, 320–326 (2013). https://doi.org/10.1007/s11418-012-0685-4

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  • DOI: https://doi.org/10.1007/s11418-012-0685-4

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