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Antimicrobial resistance in Gram-positive bacteria from Timorese River Buffalo (Bubalus bubalis) skin microbiota

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Abstract

The Timorese River Buffalo (Bubalus bubalis) plays a major role in the East Timor economy, as it is an important source of animal protein in human nutrition. They are widely spread throughout the country and are in direct contact with the populations. In spite of this proximity, information on their microbiota is scarce. This work aimed at characterizing the skin microbiota of the East Timorese River Buffalo and its antimicrobial resistance profile. Skin swab samples were taken from 46 animals in surveys conducted in three farms located in “Suco de Nairete”, Lospalos district, during July and August 2006. Bacteria were isolated and identified according to conventional microbiological procedures. A total of 456 isolates were obtained, including Gram-positive (n = 243) and Gram-negative (n = 213) bacteria. Due to their importance as potential pathogens and as vehicles for antimicrobial resistance transmission, Gram-positive cocci (n = 27) and bacilli (n = 77) isolates were further characterized, and their antimicrobial resistance profile determined by the disk diffusion method according to the Clinical and Laboratory Standards Institute guidelines. This study shows the high bacterial diversity of B. bubalis skin microbiota, representing an important first step towards understanding its importance and epidemiologic role in animal health. It also points out the potential role of these animals as vectors of antimicrobial resistant bacteria dissemination and the importance of antimicrobial resistance monitoring in developing countries.

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Acknowledgements

We would like to thank “Intercooperação e Desenvolvimento” (INDE), a non-governmental organization that fights against extreme poverty in developing countries and especially to its local coordinator, Frederico da Costa Henriques, for the sample collection; to “Direcção Geral de Veterinária” (DGV) for allowing the transport of the samples to Portugal; and to “Centro de Investigação Interdisciplinar em Sanidade Animal” (CIISA) from “Faculdade de Medicina Veterinária da Universidade Técnica de Lisboa” (FMV/UTL) for the financial support. M. Oliveira is a “Fundação para a Ciência e Tecnologia” (FCT) funded scientist from program “Ciência 2007”.

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Authors and Affiliations

  1. CIISA/Laboratório de Microbiologia e Imunologia, Faculdade de Medicina Veterinária, Universidade Técnica de Lisboa, Avenida da Universidade Técnica, 1300-477, Lisboa, Portugal

    Manuela Oliveira & Cristina L. Vilela

  2. INDE-Intercooperação e Desenvolvimento, Presently at Liga para a Protecção da Natureza, Lisboa, Portugal

    José L. Monteiro

  3. Escola Superior de Biotecnologia/AESBUC-ETGI, Sintra, Portugal

    Sílvia Rana

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  1. Manuela Oliveira
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  2. José L. Monteiro
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  3. Sílvia Rana
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  4. Cristina L. Vilela
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Correspondence to Manuela Oliveira.

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Oliveira, M., Monteiro, J.L., Rana, S. et al. Antimicrobial resistance in Gram-positive bacteria from Timorese River Buffalo (Bubalus bubalis) skin microbiota. Trop Anim Health Prod 42, 833–839 (2010). https://doi.org/10.1007/s11250-009-9494-5

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  • DOI: https://doi.org/10.1007/s11250-009-9494-5

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