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Muscle fiber types composition and type identified endplate morphology of forepaw intrinsic muscles in the rat

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Abstract

The failure to accept reinnervation is considered to be one of the reasons for the poor motor functional recovery of intrinsic hand muscles (IHMs) after nerve injury. Rat could be a suitable model to be used in simulating motor function recovery of the IHMs after nerve injury as to the similarities in function and anatomy of the muscles between human and rat. However, few studies have reported the muscle fiber types composition and endplate morphologic characteristics of intrinsic forepaw muscles (IFMs) in the rat. In this study, the myosin heavy chain isoforms and acetylcholine receptors were stained by immunofluorescence to show the muscle fiber types composition and endplates on type-identified fibers of the lumbrical muscles (LMs), interosseus muscles (IMs), abductor digiti minimi (AM) and flexor pollicis brevis (FM) in rat forepaw. The majority of IFMs fibers were labeled positively for fast-switch fiber. However, the IMs were composed of only slow-switch fiber. With the exception of the IMs, the other IFMs had a part of hybrid fibers. Two-dimensional morphological characteristics of endplates on I and IIa muscle fiber had no significant differences among the IFMs. The LMs is the most suitable IFMs of rat to stimulate reinnervation of the IHMs after nerve injury. Gaining greater insight into the muscle fiber types composition and endplate morphology in the IFMs of rat may help understand the pathological and functional changes of IFMs in rat model stimulating reinnervation of IHMs after peripheral nerve injury.

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Authors and Affiliations

  1. Department of Hand Surgery, Wuxi Number 9 People’s Hospital Affiliated to Soochow University, Wuxi, 214062, Jiangsu, People’s Republic of China

    Feng Pan, Jing-yi Mi, Yan Zhang, Xiao-yun Pan & Yong-jun Rui

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  1. Feng Pan
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  2. Jing-yi Mi
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  3. Yan Zhang
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  4. Xiao-yun Pan
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  5. Yong-jun Rui
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Correspondence to Yong-jun Rui.

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Pan, F., Mi, Jy., Zhang, Y. et al. Muscle fiber types composition and type identified endplate morphology of forepaw intrinsic muscles in the rat. J Muscle Res Cell Motil 37, 95–100 (2016). https://doi.org/10.1007/s10974-016-9450-8

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  • DOI: https://doi.org/10.1007/s10974-016-9450-8

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