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In vitro maturation of ovine oocytes using different maturation media: effect of human menopausal serum

  • Gamete Biology
  • Published:
Journal of Assisted Reproduction and Genetics Aims and scope Submit manuscript

Abstract

Objective

To compare the effect of human menopausal serum with estrous sheep serum, estrous goat serum, ovine follicular fluid and bovine follicular fluid on in vitro maturation, in vitro fertilization and embryo development of sheep oocytes

Method (s)

Oocytes were treated in culture with different sera and follicular fluids supplemented media to examine effects on embryo development.

Results

Basic culture medium supplemented with human menopausal serum, estrous sheep serum and estrous goat serum supported better rates of in vitro maturation, in vitro fertilization and embryo development. Ovine follicular fluid and bovine follicular fluid supplementations supported similar rates of In vitro maturation, In vitro fertilization and embryo development which were lower than those supported by human menopausal serum, estrous sheep serum, estrous goat serum and control medium.

Conclusion

Human menopausal serum, estrous sheep serum, and estrous goat serum resulted in higher maturation, fertilization and embryo development than ovine follicular fluid, bovine follicular fluid and control media.

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Author information

Authors and Affiliations

  1. Department of Animal Science, Razi University, Kermanshah, Iran

    H. Karami Shabankareh

  2. Animal Reproduction Physiology, Department of Animal Science, Razi University, Kermanshah, Iran

    K. Sarsaifi

  3. Department of Anatomy, Medical Faculty, University of Medical Science, Kermanshah, Iran

    T. Mehrannia

Authors
  1. H. Karami Shabankareh
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  2. K. Sarsaifi
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  3. T. Mehrannia
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Corresponding author

Correspondence to H. Karami Shabankareh.

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Capsule

Media Supplemented with human menopausal serum, estrous sheep serum and estrous goat serum supported better rates of in vitro maturation, in vitro fertilization and embryo development.

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Karami Shabankareh, H., Sarsaifi, K. & Mehrannia, T. In vitro maturation of ovine oocytes using different maturation media: effect of human menopausal serum. J Assist Reprod Genet 28, 531–537 (2011). https://doi.org/10.1007/s10815-010-9523-3

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  • DOI: https://doi.org/10.1007/s10815-010-9523-3

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