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High levels of genetic and genotypic diversity in field populations of the barley pathogen Ramularia collo-cygni

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Abstract

The ascomycete pathogen Ramularia collo-cygni causes Ramularia leaf spot (RLS) on barley. Although R. collo-cygni is considerd an emerging disease of barley, little is known about genetic diversity or population genetic structure of this pathogen. We applied a set of polymorphic AFLP (Amplified Fragment Length Polymorphism) markers to investigate population genetic structure in two Northern European populations of R. collo-cygni. The distribution of AFLP alleles revealed low levels of population subdivision and high levels of genetic diversity at both locations. Our analyses included 87 isolates and of these 84 showed a unique genotype pattern. The genetic structure of populations in Scotland and Denmark is highly similar and we find no evidence of population sub-division. An analysis of molecular variance was used to show that 86 % of the variance is attributable to within field genetic variance. In spite of the high levels of genetic and genotypic diversity in the R. collo-cygni populations, we find significant evidence of linkage disequilibrium among the AFLP alleles using a multilocus analysis. We propose that the high levels of genotypic diversity and the lack of population differentiation result from considerable levels of gene flow between populations most likely mediated by seed borne dispersal of inoculum.

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References

  • Agapow, P. M., & Burt, A. (2001). Indices of multilocus linkage disequilibrium. Molecular Ecology Notes, 1, 101–102.

    Article  CAS  Google Scholar 

  • Cavara, F. (1893). Zeitschrift für Pflanzenkrankheiten, 3, 16–19.

    Google Scholar 

  • Christiansen, M. J., Feenstra, B., Skovgaard, I. M., & Andersen, S. B. (2006). Genetic analysis of resistance to yellow rust in hexaploid wheat using a mixture model for multiple crosses. Theoretical and Applied Genetics, 112, 581–591.

    Article  PubMed  CAS  Google Scholar 

  • Eriksen, L., Shaw, M. W., & Østergård, H. (2001). A model of the effect of pseudothecia on genetic recombination and epidemic development in populations of Mycosphaerella graminicola. Phytopathology, 91, 240–248.

    Article  PubMed  CAS  Google Scholar 

  • Garcia-Vallvè, S., Palau, J., & Romeu, A. (1999). Horizontal gene transfer in glycosyl hydrolases inferred from codon usage in Escherichia coli and Bacillus subtilis. Molecular Biology and Evolution, 16, 1125–1134.

    Article  PubMed  Google Scholar 

  • Greif, P. (2002). Importance of leaf spot Ramularia collo-cygni for barley growers and breeders. Meeting the challenges of barley blights. Proceedings of the II international workshop on barley leaf blights, 7–11.

  • Halama, P. (2002). Mating relationships between isolates of Phaeosphaeria nodorum, (anamorph Stagonospora nodorum) from geographical locations. European Journal of Plant Pathology, 108, 593–596.

    Article  Google Scholar 

  • Harvey, I. (2002). Epidemiology and control of leaf and awn spot of barley caused by Ramularia collo-cygni. New Zealand Plant Protection, 55, 331–335.

    Google Scholar 

  • Leisova-Svobodova, L., Matusinsky, P., & Kucera, L. (2012). Variability of the Ramularia collo-cygni population in Central Europe. Journal of Phytopathology, 160, 701–709.

    Article  Google Scholar 

  • Linde, C. C., Zala, M., & McDonald, B. A. (2009). Molecular evidence for recent founder populations and human-mediated migration in the barley scald pathogen Rhynchosporium secalis. Molecular Phylogenetics and Evolution, 51, 454–464.

    Article  PubMed  CAS  Google Scholar 

  • Matusinsky, P., Leisova-Svobodova, L., Marik, P., Tvaruzek, L., Stemberkova, L., Hanusova, M., Minarikova, V., Vysohlidova, M., & Spitzer, T. (2011). Frequency of a mutant allele of cytochrome b conferring resistance to QoI fungicides in the Czech population of Ramularia collo-cygni. Journal for Plant Diseases and Plant Protection, 6, 248–252.

    Google Scholar 

  • McDonald, B. A., & Linde, C. (2002). Pathogen population genetics, evolutionary potential, and durable resistance. Annual Review of Phytopathology, 40, 349–379.

    Article  PubMed  CAS  Google Scholar 

  • Mueller, U., & Wolfenbarger, L. (1999). AFLP genotyping and fingerprinting. Trends in Ecology & Evolution, 14, 389–394.

    Article  Google Scholar 

  • Nei, M. (1973). Analysis of gene diversity in subdivided populations. Proceedings of the National Academy of Sciences, 70, 3321–3323.

    Article  CAS  Google Scholar 

  • Nei, M. (1978a). Estimation of average heterozygosity and genetic distance from a small number of individuals. Genetics, 89, 583–590.

    PubMed  CAS  Google Scholar 

  • Nei, M. (1978b). The theory of genetic distance and evolution of human races. Journal of Human Genetics, 23, 341–369.

    Article  CAS  Google Scholar 

  • Peakall, R., & Smouse, P. E. (2006). GENALEX 6: genetic analysis in Excel. Population genetic software for teaching and research. Molecular Ecology Notes, 6, 288–295.

    Article  Google Scholar 

  • Peakall, R., Smouse, P. E., & Huff, D. R. (1995). Evolutionary implications of allozyme and RAPD variation in diploid populations of dioecious buffalograss Buchloë dactyloides. Molecular Ecology, 4, 135–147.

    Article  CAS  Google Scholar 

  • Rau, D., Maier, F. J., Papa, R., Brown, A. H. D., Balmas, V., Saba, E., Schaefer, W., & Attene, G. (2005). Isolation and characterization of the mating-type locus of the barley pathogen Pyrenophora teres and frequencies of mating-type idiomorphs within and among fungal populations collected from barley landraces. Genome, 48, 855–869.

    Article  PubMed  CAS  Google Scholar 

  • Sachs, E. (2006). The history of research into Ramularia leaf spot on barley. Proceedings of the 1st European Ramularia Workshop, Göttingen. Germany, 9–15.

  • Schützendübel, A., Stadler, M., Wallner, D., & Von Tiedemann, A. (2008). A hypothesis on physiological alterations during plant ontogenesis governing susceptibility of winter barley to ramularia leaf spot. Plant Pathology, 57, 518–526.

    Article  Google Scholar 

  • Stabentheiner, E., Minihofer, T., & Huss, H. (2009). Infection of barley by Ramularia collo-cygni: scanning electron microscopic investigations. Mycopathologia, 168, 135–143.

    Article  PubMed  Google Scholar 

  • Vos, P., Hogers, R., Bleeker, M., Reijans, M., van de Lee, T., Hornes, M., Friters, A., Pot, J., Paleman, J., Kuiper, M., & Zabeau, M. (1995). AFLP: a new technique for DNA fingerprinting. Nucleic Acids Research, 23, 4407–4414.

    Article  PubMed  CAS  Google Scholar 

  • Walters, D. R., Havis, N. D., & Oxley, S. J. (2008). Ramularia collo-cygni: the biology of an emerging pathogen of barley. FEMS Microbiology Letters, 279, 1–7.

    Article  PubMed  CAS  Google Scholar 

  • Zaffarano, P. L., McDonald, B. A., & Linde, C. C. (2009). Phylogeographical analyses reveal global migration patterns of the barley scald pathogen Rhynchosporium secalis. Molecular Ecology, 18, 279–293.

    Article  PubMed  CAS  Google Scholar 

  • Zhan, J., Kema, G. H. J., Waalwijk, C., & McDonald, B. A. (2002). Distribution of mating type alleles in the wheat pathogen Mycosphaerella graminicola over spatial scales from lesions to continents. Fungal Genetics and Biology, 36, 128–136.

    Article  PubMed  CAS  Google Scholar 

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Acknowledgments

Sejet Plantbreeding I/S, Denmark funded the population genetic study of R. collo-cygni and a PhD fellowship to RLH.

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Authors and Affiliations

  1. Sejet Plantbreeding, Nørremarksvej 67, 8700, Horsens, Denmark

    R. L. Hjortshøj

  2. Department of Molecular Biology, Aarhus University, Gustav Wieds Vej 10c, 8000, Aarhus, Denmark

    R. L. Hjortshøj & J. Stougaard

  3. Department of Plant Biology and Biotechnology, Copenhagen University, Thorvaldsensvej 40, 1871, Frederiksberg, Denmark

    A. R. Ravnshøj

  4. Crop and Soil Research Group, Scottish Rural University College, W. Mains Road, Edinburgh, UK

    M. Nyman & N. Havis

  5. Department of Agriculture and Ecology, Copenhagen University, Thorvaldsensvej 40, 1871, Frederiksberg, Denmark

    J. Orabi & G. Backes

  6. Department of Integrated Pest Management, Aarhus University, Forsøgsvej 1, 4200, Slagelse, Denmark

    H. Pinnschmidt

  7. Max Planck Institute for Terrestial Micropbiology, Karl von Frischstraße 10, 35043, Marburg, Germany

    E. H. Stukenbrock

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  1. R. L. Hjortshøj
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  2. A. R. Ravnshøj
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  9. E. H. Stukenbrock
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Correspondence to R. L. Hjortshøj.

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Hjortshøj, R.L., Ravnshøj, A.R., Nyman, M. et al. High levels of genetic and genotypic diversity in field populations of the barley pathogen Ramularia collo-cygni . Eur J Plant Pathol 136, 51–60 (2013). https://doi.org/10.1007/s10658-012-0137-8

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  • DOI: https://doi.org/10.1007/s10658-012-0137-8

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