Abstract
We show the application of a novel optical on-line sensor fixed in spinner flasks for the online monitoring of dissolved O2 concentrations during mammalian cell growth. Using this sensor that requires only minute changes to the flask to be made, we could determine the volumetric O2 transfer coefficient as well as O2 consumption rates. Under normal growth conditions the cells did not undergo O2 limitation. Also, the transfer of O2 from the atmosphere to the spinner flasks is influenced by the use of screw caps. The on-line measurement was further applied to determine the O2 uptake rates which can then be used to monitor the metabolic state of the cells and also for online process monitoring.
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References
Annathur GV, Pierce JL, Combs RG, Rathore AS, Banerjee A, Steinmeyer DE (2003) Improvements in spinner-flask designs for insect-cell suspension culture. Biotechnol Appl Biochem 38:15–18
Deshpande RR, Heinzle E (2004) On-line oxygen uptake rate and culture viability measurement of animal cell culture using microplates with integrated oxygen sensors. Biotechnol Lett 26:763–767
Dunn IJ, Heinzle E, Ingham J, Prenosil JE (2003) Biological reaction engineering. Dynamic modelling fundamentals with simulation exercises, 2nd edn. Wiley-VCH, Weinheim
Eyer K, Heinzle E (1996) On-line estimation of ATP and biomass production in hybridoma culture at various dissolved oxygen levels. Biotechnol Bioeng 49:277–283
Eyer K, Oeggerli A, Heinzle E (1995) On-line gas analysis in animal cell cultivation: II. Oxygen uptake rate measurement and its application to controlled feeding of glutamate. Biotechnol Bioeng 45:54–62
Hanson MA, Ge X, Kostov Y, Brorson KA, Moreira AR, Rao G (2007) Comparisons of optical pH and dissolved oxygen sensors with traditional electrochemical probes during mammalian cell culture. Biotechnol Bioeng 97:833–841
Klimant I, Wolfbeis OS (1995) Oxygen-sensitive luminescent materials based on silicone-soluble ruthenium diimine complexes. Anal Chem 67:3160–3166
Kocincová AS, Nagl S, Arain S, Krause C, Borisov SM, Arnold M, Wolfbeis OS (2008) Multiplex bacterial growth monitoring in 24-well microplates using a dual optical sensor for dissolved oxygen and pH. Biotechnol Bioeng 100:430–438
Oeggerli A, Eyer K, Heinzle E (1995) On-line gas analysis in animal cell cultivation: I. Control of dissolved oxygen and pH. Biotechnol Bioeng 45:42–53
Wittmann C, Kim HM, John G, Heinzle E (2003) Characterization and application of an optical sensor for quantification of dissolved O2 in shake-flasks. Biotechnol Lett 25:377–380
Zupke C, Sinskey AJ, Stephanopoulos G (1995) Intracellular flux analysis applied to the effect of dissolved oxygen on hybridomas. Appl Microbiol Biotechnol 44:27–36
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Deshpande, R.R., Heinzle, E. Online monitoring of oxygen in spinner flasks. Biotechnol Lett 31, 665–669 (2009). https://doi.org/10.1007/s10529-009-9919-2
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DOI: https://doi.org/10.1007/s10529-009-9919-2