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Production of thermotolerant entomopathogenic Isaria fumosorosea SFP-198 conidia in corn-corn oil mixture

  • Short Communication
  • Published:
Journal of Industrial Microbiology & Biotechnology

Abstract

Low thermotolerance of entomopathogenic fungi is a major impediment to long-term storage and effective application of these biopesticides under seasonal high temperatures. The effects of high temperatures on the viability of an entomopathogenic fungus, Isaria fumosorosea SFP-198 (KCTC 0499BP), produced on different substrates amended with various additives were explored. Ground corn was found to be superior in producing the most thermotolerant conidia compared to yellow soybean, red kidney bean, and rice in a polyethylene bag production system. Using ground corn mixed with corn oil as a substrate resulted in only 7% reduction in germination compared to ground corn alone (67% reduction) after exposure of conidia to 50°C for 2 h. Corn oil as an additive for ground corn was followed by inorganic salts (KCl and NaCl), carbohydrates (sucrose and dextrin), a sugar alcohol (sorbitol), and plant oils (soybean oil and cotton seed oil) in ability to improve conidial thermotolerance. Unsaturated fatty acids, such as linoleic acid and oleic acid, the main components of corn oil, served as effective additives for conidial thermotolerance in a dosage-dependant manner, possibly explaining the improvement by corn oil. This finding suggests that the corn-corn oil mixture can be used to produce highly thermotolerant SFP-198 conidia and provides the relation of unsaturated fatty acids as substrates with conidial thermotolerance.

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Acknowledgments

This study was supported by the Biopesticide Research and Development Project of Dongbu HiTek Co. Ltd., Republic of Korea, as well as a grant from the “Specific Joint Agricultural Research-promoting Projects (project no. 20070101033014),” RDA, Republic of Korea, and the Brain Korea 21 project.

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Authors and Affiliations

  1. Entomology Research Laboratory, Department of Plant and Soil Science, University of Vermont, 661 Spear Street, Burlington, VT, 05405-0105, USA

    Jae Su Kim

  2. Dongbu Advanced Research Institute, Dongbu HiTek Co. Ltd., Daejon, 305-708, Republic of Korea

    Jae Su Kim

  3. Department of Agricultural Biotechnology, College of Agriculture and Life Sciences, Seoul National University, Seoul, 151-742, Republic of Korea

    Yeon Ho Je & Jong Yul Roh

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  1. Jae Su Kim
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  2. Yeon Ho Je
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Correspondence to Jae Su Kim.

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Kim, J.S., Je, Y.H. & Roh, J.Y. Production of thermotolerant entomopathogenic Isaria fumosorosea SFP-198 conidia in corn-corn oil mixture. J Ind Microbiol Biotechnol 37, 419–423 (2010). https://doi.org/10.1007/s10295-010-0692-y

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  • DOI: https://doi.org/10.1007/s10295-010-0692-y

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