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Effects of LLLT in combination with bisphosphonate on bone healing in critical size defects: a histological and histometric study in rat calvaria

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Abstract

The purpose of this study was to analyze histologically the effect of low-level laser therapy (LLLT) in combination with bisphosphonate on bone healing in surgically created critical size defects (CSD) in rat calvaria. One hundred Wistar female rats sham operated (sham) and ovariectomized (Ovx) were maintained untreated for 1 month to allow for the development of osteopenia in the Ovx animals. A CSD was made in the calvarium of each rat, and the animals were divided into five groups according to following treatments: (1) sham rats (control), (2) Ovx rats, (3) Ovx rats treated with LLLT, (4) Ovx rats treated with bisphosphonate, and (5) Ovx rats treated with bisphosphonate and LLLT. Groups 4 and 5 were irrigated with 1 ml of bisphosphonate, and groups 3 and 5 were submitted to LLLT (GaAlAs), 660 nm, 24 J, and 0.4285 W/cm2 on the CSD. Ten animals of each treatment were killed at 30 and 60 days. Histomorphometric assessments, using image analysis software, and histological analyses were performed. No defect was completely regenerated with the bone. Histometrically, it can be observed that groups 3 (37.49 ± 1.94%, 43.11 ± 2.39%) and 5 (35.05 ± 1.57%, 41.07 ± 1.89%) showed a significant bone neoformation when compared to groups 1 (16.81 ± 1.57%, 27.54 ± 1.49%), 2 (11.68 ± 0.98%, 22.51 ± 1.05%), and 4 (14.62 ± 1.70%, 25.67 ± 1.41%) in all experimental periods (P < 0.05). It was possible to conclude that the LLLT associated or not with bisphosphonate treatment was effective for stimulating bone formation in CSD in the calvaria of rats submitted to ovariectomy.

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Acknowledgments

Juliana Mendonça da Conceição received a scholarship from the São Paulo State Foundation for Research (FAPESP: 2007/55072-5). This paper is attributed to the Department of Periodontology, Araçatuba Dental School, São Paulo State University (UNESP) Araçatuba, São Paulo, Brazil.

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Authors and Affiliations

  1. GEPLO—Group of Study and Research on Laser in Dentistry, Department of Surgery and Integrated Clinic—Division of Periodontics, São Paulo State University (UNESP)—UNESP, Araçatuba, São Paulo, Brazil

    Valdir Gouveia Garcia, Juliana Mendonça da Conceição, Leandro Araújo Fernandes, Juliano Milanezi de Almeida, Maria José Hitomi Nagata, Alvaro Francisco Bosco & Leticia Helena Theodoro

  2. Department of Clinic and Surgery, Federal University of Alfenas, Alfenas, Minas Gerais, Brazil

    Leandro Araújo Fernandes

  3. Department of Periodontics, University Center of the Educational Foundation of Barretos (UNIFEB), Barretos Dental School, Barretos, São Paulo, Brazil

    Valdir Gouveia Garcia

  4. Faculdade de Odontologia de Araçatuba—UNESP, Rua Jose Bonifácio 1193, Centro, CEP 16050-300, Araçatuba, São Paulo, Brazil

    Valdir Gouveia Garcia

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  1. Valdir Gouveia Garcia
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  2. Juliana Mendonça da Conceição
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Correspondence to Valdir Gouveia Garcia.

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Garcia, V.G., da Conceição, J.M., Fernandes, L.A. et al. Effects of LLLT in combination with bisphosphonate on bone healing in critical size defects: a histological and histometric study in rat calvaria. Lasers Med Sci 28, 407–414 (2013). https://doi.org/10.1007/s10103-012-1068-5

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  • DOI: https://doi.org/10.1007/s10103-012-1068-5

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