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Impact of ambient and supplemental ultraviolet-B stress on kidney bean plants: an insight into oxidative stress management

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Abstract

In the present study, the response of kidney bean (Phaseolus vulgaris L. cv. Pusa Komal) plants was evaluated under three different levels of ultraviolet-B (UV-B), i.e., excluded UV-B (eUV-B), ambient UV-B (aUV-B; 5.8 kJ m−2 day−1), and supplemental UV-B (sUV-B; 280–315 nm; ambient + 7.2 kJ m−2 day−1), under near-natural conditions. eUV-B treatment clearly demonstrated that both aUV-B and sUV-B are capable of causing significant changes in the plant’s growth, metabolism, economic yield, genome template stability, total protein, and antioxidative enzyme profiles. The experimental findings showed maximum plant height at eUV-B, but biomass accumulation was minimum. Significant reductions in quantum yield (Fv/Fm) were observed under both aUV-B and sUV-B, as compared to eUV-B. UV-B-absorbing flavonoids increased under higher UV-B exposures with consequent increments in phenylalanine ammonia lyase (PAL) activities. The final yield was significantly higher in plants grown under eUV-B, compared to those under aUV-B and sUV-B. Total protein profile through sodium dodecyl sulfate-polyacrylamide gel electrophoresis (SDS-PAGE) and analysis of isoenzymes, like superoxide dismutase (SOD), peroxidase (POX), catalase (CAT), ascorbate peroxidase (APX), guaiacol peroxidase (GPX), and glutathione reductase (GR), through native PAGE revealed major changes in the leaf proteome under aUV-B and sUV-B, depicting induction of some major stress-related proteins. The random amplified polymorphic DNA (RAPD) profile of genomic DNA also indicated a significant reduction of genome template stability under UV-B exposure. Thus, it can be inferred that more energy is diverted for inducing protection mechanisms rather than utilizing it for growth under high UV-B level.

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Acknowledgments

We thank the Head of the Department of Botany and the Co-ordinator of CAS in Botany, Banaras Hindu University, for providing the necessary laboratory and field facilities. The authors are also thankful to the authorities of the Council of Scientific and Industrial Research, New Delhi, for providing financial assistance to SS as RA and AS as SRF.

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The authors declare that they have no conflict of interest.

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  1. Laboratory of Air Pollution and Global Climate Change, Department of Botany, Banaras Hindu University, Varanasi, 221005, India

    Suruchi Singh, Abhijit Sarkar, S.B. Agrawal & Madhoolika Agrawal

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  1. Suruchi Singh
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  4. Madhoolika Agrawal
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Correspondence to Madhoolika Agrawal.

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Singh, S., Sarkar, A., Agrawal, S. et al. Impact of ambient and supplemental ultraviolet-B stress on kidney bean plants: an insight into oxidative stress management. Protoplasma 251, 1395–1405 (2014). https://doi.org/10.1007/s00709-014-0641-0

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