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Diagnostic and prognostic value of immunohistochemical expression of S100P and IMP3 in transpapillary biliary forceps biopsy samples of extrahepatic bile duct carcinoma

  • Original article
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Journal of Hepato-Biliary-Pancreatic Sciences

Abstract

Background

Because the biopsy specimen of extrahepatic bile duct carcinoma (EHBDC) is small and shows reactive changes, the histological distinction between malignant and benign tissue can be difficult. Recent studies reported that S100P and insulin-like growth factor II mRNA-binding protein 3 (IMP3) were not only diagnostic molecules but also prognostic biomarkers in several organs. The objective of this study is to clarify the diagnostic and prognostic value of immunohistochemical expression of S100P and IMP3 in transpapillary biliary forceps biopsy (TBFB) samples.

Methods

The TBFB samples were collected from 80 patients (EHBDC, 68 patients; benign, 12 patients), retrospectively.

Results

When using cytoplasmic-positive staining for IMP3 as a marker of malignancy, the sensitivity and specificity reached 79.4 and 91.7 %, respectively. The sensitivity, specificity and accuracy achieved 89.7, 91.7 and 90.0 %, respectively, when using positive staining for IMP3 and/or positive histology as a maker of malignancy. While univariate (P = 0.033) and multivariate (P = 0.039) analysis revealed that S100P-positive EHBDC patients showed significantly shorter survival.

Conclusions

The results of this study suggest that immunohistochemical staining for IMP3 is useful in the diagnosis of EHBDC and that of S100P is useful as a prognostic marker for EHBDC.

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Acknowledgments

This work was supported by Grant-in Aid for Young Scientists (B) 22790525 from the Ministry of Education, Culture, Sports, Science and Technology of Japan. Additionally, the authors wish to thank Yuya Itoh, MD, Yosuke Nakamura, MD, Takeshi Hiramatsu, MD, Hiroyuki Sugimoto, MD, Hajime Sumi, MD and Daijuro Hayashi, MD for their contributions.

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Authors and Affiliations

  1. Department of Gastroenterology and Hepatology, Nagoya University Graduate School of Medicine, Nagoya, Japan

    Hiroki Kawashima, Akihiro Itoh, Ryoji Miyahara, Naoki Ohmiya, Tsutomu Tanaka & Hidemi Goto

  2. Department of Endoscopy, Nagoya University Hospital, 65 Tsuruma-cho, Showa-ku, Nagoya, 466-8550, Japan

    Eizaburo Ohno & Yoshiki Hirooka

  3. Department of Pathology and Clinical Laboratories, Nagoya University Hospital, Nagoya, Japan

    Yoshie Shimoyama & Shigeo Nakamura

  4. Division of Surgical Oncology, Department of Surgery, Nagoya University Graduate School of Medicine, Nagoya, 466-8550, Japan

    Tomoki Ebata & Masato Nagino

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  1. Hiroki Kawashima
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  2. Akihiro Itoh
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Correspondence to Yoshiki Hirooka.

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Kawashima, H., Itoh, A., Ohno, E. et al. Diagnostic and prognostic value of immunohistochemical expression of S100P and IMP3 in transpapillary biliary forceps biopsy samples of extrahepatic bile duct carcinoma. J Hepatobiliary Pancreat Sci 20, 441–447 (2013). https://doi.org/10.1007/s00534-012-0581-z

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  • DOI: https://doi.org/10.1007/s00534-012-0581-z

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