Abstract
Strain improvement of Pichia angophorae KCTC 17574 was successfully carried out for bioethanol fermentation of seaweed slurry with high salt concentration. P. angophorae KCTC 17574 was cultured under increasing salinity from five practical salinity unit (psu, ‰) to as high as 100 psu for 723 h. The seaweed, Undaria pinnatifida (sea mustard, Miyuk), was fermented to produce bioethanol using high-salt acclimated yeast. The pretreatment of U. pinnatifida was optimized using thermal acid hydrolysis to obtain a high monosaccharide yield. Optimal pretreatment conditions of 75 mM H2SO4 and 13 % (w/v) slurry at 121 °C for 60 min were determined using response surface methodology. A maximum monosaccharide content of 28.65 g/L and the viscosity of 33.19 cP were obtained. The yeasts cultured under various salinity concentrations were collected and inoculated to the pretreated seaweed slurry after the neutralization using 5 N NaOH. The pretreated slurry was fermented with the inoculation of 0.1 g dcw/L of P. angophorae KCTC 17574 strain obtained at 90 psu. The maximum ethanol concentration of 9.42 g/L with 27 % yield of theoretical case of ethanol production from total carbohydrate of U. pinnatifida was obtained.
This is a preview of subscription content, log in via an institution to check access.
Similar content being viewed by others
References
Saxena RC, Adhikari DK, Goyal HB (2009) Biomass-based energy fuel through biochemical routes: a review. Renew Sustain Energy Rev 13:167–178
Alvira P, Tomas-Pejo E, Ballesteros M, Negro MJ (2010) Pretreatment technologies for an efficient bioethanol production process based on enzymatic hydrolysis: a review. Bioresour Technol 101:4851–4861
David C, Matteo P, Simone F, Luis O, Piero O, Paolo T, Francesco C (2012) Review of pretreatment processes for lignocellulosicethanol production, and development of an innovative method. Biomass Bioeng 46:25–35
Dias MOS, Ensinas AV, Nebra SA, Maciel R, Rossell CEV, Maciel MRW (2009) Production of bioethanol and other bio-based materials from sugarcane bagasse: integration to conventional bioethanol production process. Chem Eng Res Des 87:1206–1216
Rojan PJ, Anisha GS, Madhavan N, Ashok P (2011) Micro and macroalgal biomass: a renewable source for bioethanol. Bioresour Technol 102:186–193
Ge L, Peng W, Haijin M (2011) Study on saccharification techniques of seaweed wastes for the transformation of ethanol. Renew Energy 36:84–89
Pablo EM, Carolina VM, Graciela C (2012) Occurrence of the alien kelp Undaria pinnatifida (Laminariales, Phaeophyceae) in Mar del Plata, Argentina. Bioinvasions Rec 1:59–63
Dumitriu S (1998) Polysaccharides. Markker Deccer Inc., New York
Tang JC, Taniguchi H, Chu H, Zhou H, Nagata S (2009) Isolation and characterization of alginate-degrading bacteria for disposal of seaweed wastes. Lett Appl Microbiol 48:38–43
Groleau D, Chevalier P, Tse Hing Yuen TLS (1995) Production of polyols and ethanol by the osmophilic yeast Zygosaccharomyces rouxii. Biotechnol Lett 3:315–320
Khroustalyova K, Adler L, Rapoport A (2001) Exponential growth phase cells of the osmotolerant yeast Debaryomyces hansenii are extremely resistant to dehydration stress. Process Biochem 36:1163–1166
AOAC (Association of Official Analytical Chemists) (1995) In: Cunniff P (ed) Official methods of analysis of the association of official analytical chemists, 16th edn. Association of Officail Analytical Chemists, Arlington, VA
Redding AP, Wang Z, Keshwani DR, Cheng J (2010) High temperature dilute acid pretreatment of coastal Bermuda grass for enzymatic hydrolysis. Bioresour Technol 102:1415–1424
Lu X, Zhang Y, Angelidaki I (2009) Optimization of H2SO4-catalyzed hydrothermal pretreatment of rapeseed straw for bioconversion to ethanol: focusing on pretreatment at high solids content. Bioresour Technol 100:3048–3053
Rosgaard L, Andric P, Dam-Johansen K, Pedersen S, Meyer AS (2007) Effects of substrate loading on enzymatic hydrolysis and viscosity of pretreated barley straw. Appl Biochem Biotechnol 143:27–40
Nkemka VN, Murto M (2010) Evaluation of biogas production from seaweed in batch tests and in UASB reactors combined with the removal of heavy metals. J Environ Manage 91:1573–1579
Klinke HB, Thomsen AB, Ahring BK (2004) Inhibition of ethanol-producing yeast and bacteria by degradation products produced during pre-treatment of biomass. Appl Microbiol Biotechnol 66:10–26
Horn SJ, Aasen IM, Ostgaard K (2000) Ethanol production from seaweed extract. J Ind Microbiol 25:249–254
Taherzadeh MJ, Niklasson C, Liden G (1997) Acetic acid-friend or foe in anaerobic batch conversion of glucose to ethanol by Saccharomyces cerevisiae. Chem Eng Sci 52:2653–2659
Larsson S, Palmqvist E, Hahn-Hagerdal B, Tengborg C, Stenberg K, Zacchi G, Nilvebrant NO (1999) The generation of fermentation inhibitors during dilute acid hydrolysis of softwood. Enz Microbiol Technol 24:151–159
Oliva JM, Negro MJ, Saez F, Ballesteros I, Manzanares P, Gonzalez A, Ballesteros M (2006) Effects of acetic acid, furfural and catechol combinations on ethanol fermentation of Kluyveromyces marxianus. Process Biochem 41:1223–1228
Adam JW, Effendi L, Maung NW, Drew DR, Christine NS, Peter BK, Susan RC, Ryan MR, Asael H, Alicia BS, Arun L, Yuki K, David B, Yasuo Y (2012) An engineered microbial platform for direct biofuel production from brown macroalgae. Science 335:308–313
Acknowledgments
The research was supported by a grant from Marine Biotechnology Program Funded by Ministry of Land, Transport and Maritime Affairs of Korean Government.
Author information
Authors and Affiliations
Corresponding author
Rights and permissions
About this article
Cite this article
Cho, Y., Kim, H. & Kim, SK. Bioethanol production from brown seaweed, Undaria pinnatifida, using NaCl acclimated yeast. Bioprocess Biosyst Eng 36, 713–719 (2013). https://doi.org/10.1007/s00449-013-0895-5
Received:
Accepted:
Published:
Issue Date:
DOI: https://doi.org/10.1007/s00449-013-0895-5