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Improving the efficiency of enzyme utilization for sugar beet pulp hydrolysis

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Abstract

Sugar beet pulp (SBP) is a carbohydrate-rich residue of table sugar processing. It shows promise as a feedstock for fermentable sugar and biofuel production via enzymatic hydrolysis and microbial fermentation. This research focused on the enzymatic hydrolysis of SBP and examined the effects of solid loading (2–10 %, dry basis), enzyme preparation, and enzyme recycle on the production of fermentable sugars. The enzyme partitioning to the solid and liquid phases during SBP enzymatic hydrolysis and loss during recycling were investigated using SDS-PAGE and Zymogram analysis. Without considering product inhibition, the cellulase added initially to the SBP hydrolysis lost only 6 % filter paper activity and negligible carboxymethyl cellulose activity upon multiple cycles of SBP hydrolysis. It was found that enzyme dosage can be reduced by 50 % while maintaining similar, and in some cases higher fermentable sugar yield. The removal of hydrolysis products will further improve enzymatic hydrolysis of SBP for biofuel production.

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Acknowledgments

The funding for this research was provided by Chevron Technology Ventures.

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Author notes

  1. Yi Zheng

    Present address: Novozymes A/S, Krogshoejvej 36, 2880, Bagsvaerd, Denmark

Authors and Affiliations

  1. Department of Biological and Agricultural Engineering, University of California, Davis One Shields Avenue, Davis, CA, 95616, USA

    Yi Zheng, Yu-Shen Cheng, Chaowei Yu, Ruihong Zhang, Bryan M. Jenkins & Jean S. VanderGheynst

  2. Chemical and Material Engineering, National Yunlin University of Science and Technology, Yunlin, 64002, Taiwan

    Yu-Shen Cheng

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  1. Yi Zheng
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Correspondence to Jean S. VanderGheynst.

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Zheng, Y., Cheng, YS., Yu, C. et al. Improving the efficiency of enzyme utilization for sugar beet pulp hydrolysis. Bioprocess Biosyst Eng 35, 1531–1539 (2012). https://doi.org/10.1007/s00449-012-0743-z

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