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Kinetics of hydrogen peroxide decomposition by catalase: hydroxylic solvent effects

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Abstract

The effect of water–alcohol (methanol, ethanol, propan-1-ol, propan-2-ol, ethane-1,2-diol and propane-1,2,3-triol) binary mixtures on the kinetics of hydrogen peroxide decomposition in the presence of bovine liver catalase is investigated. In all solvents, the activity of catalase is smaller than in water. The results are discussed on the basis of a simple kinetic model. The kinetic constants for product formation through enzyme–substrate complex decomposition and for inactivation of catalase are estimated. The organic solvents are characterized by several physical properties: dielectric constant (D), hydrophobicity (log P), concentration of hydroxyl groups ([OH]), polarizability (α), Kamlet-Taft parameter (β) and Kosower parameter (Z). The relationships between the initial rate, kinetic constants and medium properties are analyzed by linear and multiple linear regression.

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Acknowledgments

Dr. Mihaela Puiu is grateful to the strategic grant POSDRU/89/1.5/S/58852 Project “Postdoctoral programme for training young scientific researchers” co-financed by the European Social Found within the Sectorial Operational Program Human Resources Development 2007–2013 for the fellowship supporting this research.

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Authors and Affiliations

  1. Department of Physical Chemistry, Faculty of Chemistry, University of Bucharest, Bd. Regina Elisabeta, Nr. 4–12, 030018, Bucharest, Romania

    Adina Raducan, Anca Ruxandra Cantemir & Dumitru Oancea

  2. LaborQ, University of Bucharest, Bd. Regina Elisabeta, Nr. 4–12, 030018, Bucharest, Romania

    Mihaela Puiu

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  1. Adina Raducan
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  2. Anca Ruxandra Cantemir
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Correspondence to Adina Raducan.

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Raducan, A., Cantemir, A.R., Puiu, M. et al. Kinetics of hydrogen peroxide decomposition by catalase: hydroxylic solvent effects. Bioprocess Biosyst Eng 35, 1523–1530 (2012). https://doi.org/10.1007/s00449-012-0742-0

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