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Cold induced Botrytis cinerea enolase (BcEnol-1) functions as a transcriptional regulator and is controlled by cAMP

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Abstract

Botrytis cinerea is a necrotrophic fungal plant pathogen that can survive, grow and infect crops under cold stress. In an attempt to understand the molecular mechanisms leading to cold tolerance of this phytopathogen, we identified an enolase, BcEnol-1. BcEnol-1 encodes a 48 kDa protein that shows high identity to yeast, Arabidopsis and human enolases (72, 63 and 63%, respectively). Northern analysis confirms that an increase in transcript abundance of BcEnol-1 was observed when B. cinerea mycelium was shifted from 22 to 4°C. In order to understand its regulation during cold stress, BcEnol-1 expression was studied in B. cinerea mutants viz Δbcg1 (mutant of B. cinerea for bcg1), Δbcg3 (mutant of B. cinerea for bcg3) and Δbac (mutant of B. cinerea for adenylate cyclase). A decrease in enolase expression in these mutants was observed during cold stress suggesting enolase activation by a cAMP mediated cascade. Expression of enolase was restored with the exogenous addition of cAMP to the Δbac mutant. Recombinant enolase protein was also found to bind to the promoter elements of transcripts belonging to the Zinc-C6 protein family and calpain like proteases. Based on these results we conclude that enolase from Botrytis is cold responsive, influenced by cAMP and acts putatively as a transcriptional regulator.

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Acknowledgments

We are grateful to Matthias Hahn for providing the Δbcg3 mutant and for critically reading the manuscript. We are also grateful to Amnon Lichter for his inputs during the preparation of the manuscript. This work was supported by the University of Alabama in Huntsville, start-up and Faculty Minigrant Award and BARD award # IS-3947-06 to M.R.D. A.K.·P. was also supported by M.R.D. through BARD funds.

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Authors and Affiliations

  1. Department of Biological Sciences, University of Alabama in Huntsville, Huntsville, AL, 35899, USA

    Ajay K. Pandey, Preti Jain, Gopi K. Podila & Maria R. Davis

  2. Institut fur Botanik der Westfalischen Wilhelms, Universitat Munster, Schlossgarten 3, 48149, Munster, Germany

    Bettina Tudzynski

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  1. Ajay K. Pandey
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  2. Preti Jain
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  5. Maria R. Davis
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Correspondence to Maria R. Davis.

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Communicated by J. Perez-Martin.

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Pandey, A.K., Jain, P., Podila, G.K. et al. Cold induced Botrytis cinerea enolase (BcEnol-1) functions as a transcriptional regulator and is controlled by cAMP. Mol Genet Genomics 281, 135–146 (2009). https://doi.org/10.1007/s00438-008-0397-3

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