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The protective role of DJ-1 in ultraviolet-induced damage of human skin: DJ-1 levels in the stratum corneum as an indicator of antioxidative defense

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Abstract

DJ-1 is a multifunctional protein associated with Parkinson’s disease and plays a significant role in protecting nerve cells from oxidative stress. DJ-1 is expressed in the skin, although its function there is unknown. In this study, we investigated DJ-1 function in keratinocytes. DJ-1 was induced by H2O2 exposure and UV irradiation in keratinocytes. DJ-1 knockdown with small interfering RNA (siRNA) increased reactive oxygen species (ROS) and lactate dehydrogenase (LDH) release after UVB irradiation, suggesting that DJ-1 reduces ROS and might protect skin cells from UV damage in vitro. To investigate the in vivo role of DJ-1 in the skin, we determined DJ-1 levels in human stratum corneum samples obtained by the tape-stripping method. DJ-1 levels in the stratum corneum (scDJ-1) correlated with total antioxidant capacity. We also examined the effect of scDJ-1 on changes in skin after UVB irradiation. DJ-1 was elevated in SC from the upper arm 1 to 2 weeks after UVB irradiation. One day after UVB irradiation, L* (brightness) and a* (redness) values, indicators of skin color, were altered regardless of scDJ-1 expression. However, these values recovered more quickly in subjects with high scDJ-1 expression than in those with low scDJ-1 expression. These data suggest that DJ-1 in skin plays a significant role in protection against UV radiation and oxidative stress, and that DJ-1 levels in the SC might be an indicator of antioxidative defense against UV-induced damage.

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Authors and Affiliations

  1. Fancl Research Institute, Totsuka, Yokohama, 244-0806, Japan

    Shioji Ishiwatari, Minako Takahashi, Chie Yasuda, Maho Nakagawa & Shoko Matsukuma

  2. Systems Life Sciences Laboratory, Department of Medical Life Systems, Faculty of Life and Medical Sciences, Doshisha University, Kyotanabe, Kyoto, 610-0394, Japan

    Yoshiro Saito & Noriko Noguchi

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  1. Shioji Ishiwatari
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  2. Minako Takahashi
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Correspondence to Shioji Ishiwatari.

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Ishiwatari, S., Takahashi, M., Yasuda, C. et al. The protective role of DJ-1 in ultraviolet-induced damage of human skin: DJ-1 levels in the stratum corneum as an indicator of antioxidative defense. Arch Dermatol Res 307, 925–935 (2015). https://doi.org/10.1007/s00403-015-1605-8

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  • DOI: https://doi.org/10.1007/s00403-015-1605-8

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