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The uncharacterized gene 1700093K21Rik and flanking regions are correlated with reproductive isolation in the house mouse, Mus musculus

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Abstract

Reproductive barriers exist between the house mouse subspecies, Mus musculus musculus and M. m. domesticus, members of the Mus musculus species complex, primarily as a result of hybrid male infertility, and a hybrid zone exists where their ranges intersect in Europe. Using single nucleotide polymorphisms (SNPs) diagnostic for the two taxa, the extent of introgression across the genome was previously compared in these hybrid populations. Sixty-nine of 1316 autosomal SNPs exhibited reduced introgression in two hybrid zone transects suggesting maladaptive interactions among certain loci. One of these markers is within a region on chromosome 11 that, in other studies, has been associated with hybrid male sterility of these subspecies. We assessed sequence variation in a 20 Mb region on chromosome 11 flanking this marker, and observed its inclusion within a roughly 150 kb stretch of DNA showing elevated sequence differentiation between the two subspecies. Four genes are associated with this genomic subregion, with two entirely encompassed. One of the two genes, the uncharacterized 1700093K21Rik gene, displays distinguishing features consistent with a potential role in reproductive isolation between these subspecies. Along with its expression specifically within spermatogenic cells, we present various sequence analyses that demonstrate a high rate of molecular evolution of this gene, as well as identify a subspecies amino acid variant resulting in a structural difference. Taken together, the data suggest a role for this gene in reproductive isolation.

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Acknowledgments

This work was funded by an Eastern Michigan University Sabbatical Leave Award (DHK) and an NSF-Research Opportunity Award (ROA) 0746560 to PKT. VJ received support from the Institutional Research Support Grant No. SVV-2013-267 201, the Grant Agency of Charles University in Prague Grant No. 6421/2012 and from the NextGen project No. CZ.1.07/2.3./20.0303. The authors thank Miloš Macholán, Jaroslav Piálek, Michael Nachman, and two anonymous reviewers for their valuable comments on an earlier version of this manuscript. The authors also thank Michael Nachman for providing us transcriptome data prior to publication.

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Authors and Affiliations

  1. Department of Biology, Eastern Michigan University, 441 Mark Jefferson, Ypsilanti, MI, 48197, USA

    David H. Kass

  2. Department of Zoology, Faculty of Science, Charles University in Prague, Viničná 7, 128 43, Prague 2, Czech Republic

    Václav Janoušek

  3. Department of Population Biology, Institute of Vertebrate Biology, ASCR, Studenec 123, 675 02 Koněšín and Květná 8, 60365, Brno, Czech Republic

    Václav Janoušek

  4. Department of Ecology and Evolutionary Biology and Museum of Zoology, University of Michigan, Ann Arbor, MI, 48109, USA

    Liuyang Wang & Priscilla K. Tucker

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  1. David H. Kass
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  2. Václav Janoušek
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  4. Priscilla K. Tucker
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Correspondence to Priscilla K. Tucker.

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Kass, D.H., Janoušek, V., Wang, L. et al. The uncharacterized gene 1700093K21Rik and flanking regions are correlated with reproductive isolation in the house mouse, Mus musculus . Mamm Genome 25, 223–234 (2014). https://doi.org/10.1007/s00335-014-9506-2

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