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Impact of Food-Related Environmental Factors on the Adherence and Biofilm Formation of Natural Staphylococcus aureus Isolates

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Abstract

Staphylococcus aureus is a pathogenic bacterium capable of developing biofilms on food-processing surfaces, a pathway leading to cross contamination of foods. The purpose of this study was to investigate the influence of environmental stress factors found during seafood production on the adhesion and biofilm-forming properties of S. aureus. Adhesion and biofilm assays were performed on 26 S. aureus isolated from seafood and two S. aureus reference strains (ATCC 6538 and ATCC 43300). Cell surface properties were evaluated by affinity measurements to solvents in a partitioning test, while adhesion and biofilm assays were performed in polystyrene microplates under different stress conditions of temperature, osmolarity, and nutrient content. The expression of genes implicated in the regulation of biofilm formation (icaA, rbf and σB) was analyzed by reverse transcription and quantitative real time PCR. In general, S. aureus isolates showed moderate hydrophobic properties and a marked Lewis-base character. Initial adhesion to polystyrene was positively correlated with the ionic strength of the growth medium. Most of the strains had a higher biofilm production at 37 °C than at 25 °C, promoted by the addition of glucose, whereas NaCl and MgCl2 had a lower impact markedly affected by incubation temperatures. Principal Component Analysis revealed a considerable variability in adhesion and biofilm-forming properties between S. aureus isolates. Transcriptional analysis also indicated variations in gene expression between three characteristic isolates under different environmental conditions. These results suggested that the prevalence of S. aureus strains on food-processing surfaces is above all conditioned by the ability to adapt to the environmental stress conditions present during food production. These findings are relevant for food safety and may be of importance when choosing the safest environmental conditions and material during processing, packaging, and storage of seafood products.

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Acknowledgments

Author VSD was awarded a research grant (JAE Program) by the Spanish National Research Council (CSIC). We also thank Tone Mari Rode for her excellent scientific advice, Signe Marie Drømtorp for her technical assistance, and Verónica Asensio Fandiño for her collaboration during statistical analysis.

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Authors and Affiliations

  1. Seafood Microbiology and Technology Section, Marine Research Institute, Eduardo Cabello 6, Vigo, Pontevedra, Spain

    Daniel Vázquez-Sánchez

  2. School of Chemical and Bioprocess Engineering, University College Dublin, Dublin, Ireland

    Olivier Habimana

  3. Nofima, Ås, Norway

    Askild Holck

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  1. Daniel Vázquez-Sánchez
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  2. Olivier Habimana
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  3. Askild Holck
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Correspondence to Daniel Vázquez-Sánchez.

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Vázquez-Sánchez, D., Habimana, O. & Holck, A. Impact of Food-Related Environmental Factors on the Adherence and Biofilm Formation of Natural Staphylococcus aureus Isolates. Curr Microbiol 66, 110–121 (2013). https://doi.org/10.1007/s00284-012-0247-8

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