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The Inhibitory Effect of Platelet-Rich Plasma on Botulinum Toxin Type-A: An Experimental Study in Rabbits

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Abstract

Background

Combination treatments of botulinum toxin type-A and other rejuvenation agents or instruments are gradually becoming more popular. After observing a high incidence of therapy failure following simultaneous applications of botulinum toxin type-A and platelet-rich plasma mesotherapy, we aimed to investigate whether PRP has an inhibitory effect on botulinum toxin type-A.

Methods

Twenty-four New Zealand white rabbits were divided into 4 groups, and the anterior auricular muscle and overlying skin were used for injections. Groups I and II both received onabotulinumtoxinA intramuscular injections. In addition, autologous platelet-rich plasma mesotherapy was performed in Group I while Group II received saline mesotherapy. Group III was designed as the in vitro mixture group in which onabotulinumtoxinA and platelet-rich plasma were mixed and then administered intramuscularly. Group IV received saline within the mixture instead of platelet-rich plasma. The contralateral ears of all the rabbits served as control and were only treated with onabotulinumtoxinA. Visual evaluation of ear positions and electroneuromyographic studies were done prior to all procedures and at day 14. Anterior auricular muscles were harvested at day 14 and were evaluated with quantitative real-time PCR.

Results

Visual and electroneuromyographic studies revealed less onabotulinumtoxinA activity in Groups I and III. When platelet-rich plasma was administered through skin mesotherapy, onabotulinumtoxinA activity failure was more severe in comparison with direct contact. No significant difference in SNAP-25 mRNA expression through quantitative real-time PCR was observed between groups.

Conclusion

Although we could not explain the exact mechanism underlying this interaction, platelet-rich plasma applications result in less onabotulinumtoxinA muscle paralysis activity.

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Acknowledgments

This study was approved by the Gazi University Ethical Committee for Experimental Research on Animals (Project no: G.U. ET-43-6865) and was financially supported by the Gazi University Research Fund (Project no: 01/2012-60).

Conflict of interest

The authors declare that they have no conflicts of interest to disclose within the blinded manuscript.

Statement of Animal Rights

All applicable institutional and/or national guidelines for the care and use of animals were followed.

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Authors and Affiliations

  1. Department of Plastic Reconstructive and Aesthetic Surgery, Gazi University Faculty of Medicine, Ankara, Turkey

    Hakan Bulam, Suhan Ayhan, Billur Sezgin, Kemal Findikcioglu, Serhan Tuncer & Seyhan Cenetoglu

  2. Department of Physical Therapy and Rehabilitation, Gazi University Faculty of Medicine, Ankara, Turkey

    Murat Zinnuroglu

  3. Department of Medical Biology and Genetics, Gazi University Faculty of Medicine, Ankara, Turkey

    Ece Konac & Nuray Varol

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  1. Hakan Bulam
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  2. Suhan Ayhan
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Correspondence to Suhan Ayhan.

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Bulam, H., Ayhan, S., Sezgin, B. et al. The Inhibitory Effect of Platelet-Rich Plasma on Botulinum Toxin Type-A: An Experimental Study in Rabbits. Aesth Plast Surg 39, 134–140 (2015). https://doi.org/10.1007/s00266-014-0418-z

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  • DOI: https://doi.org/10.1007/s00266-014-0418-z

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