Abstract
The multivariate algorithm hierarchical cluster analysis is applied to sets of resonance Raman spectra collected from human erythrocytes infected with the malaria parasite Plasmodium falciparum. The images obtained yield information about the distribution of hemoglobin and hemozoin (or malaria pigment) within the parasitized cells and about their molecular structure. This method has the advantage of conveying more information than other imaging approaches based on resonance Raman spectroscopy, and it is a promising tool to study the hemozoin formation process and its interaction with antimalarial drugs within unstained, well-preserved parasites.
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Acknowledgements
V.S. and A.B. acknowledge partial financial support from BINASP/INFRAEUR and IRCCS Burlo Garofolo, respectively. The work by D.T. and S.P. was generated in the context of the AntiMal project, funded under the 6th Framework Programme of the European Community (contract no. IP-018834). S.F. is a PhD student of the AntiMal International PhD Programme run in collaboration with the European Molecular Biology Laboratory (Heidelberg). The authors thank Nicoletta Basilico and Diego Monti for useful suggestions and critical review of the data.
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Bonifacio, A., Finaurini, S., Krafft, C. et al. Spatial distribution of heme species in erythrocytes infected with Plasmodium falciparum by use of resonance Raman imaging and multivariate analysis. Anal Bioanal Chem 392, 1277–1282 (2008). https://doi.org/10.1007/s00216-008-2414-0
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DOI: https://doi.org/10.1007/s00216-008-2414-0