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Kapillarelektrophorese gekoppelte Massenspektrometrie zur Proteomanalyse

Eine innovative diagnostische Methode bei Prostata- und Blasenkrebs

Capillary electrophoresis coupled to mass spectrometry for proteome analysis

An innovative diagnostic method for prostate and bladder cancer

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Zusammenfassung

Zur nicht-invasiven Erkennung von Urothel- oder Prostatakarzinomen wurde eine Proteomuntersuchung von Urin entwickelt. Mittels Kapillarelektrophorese gekoppelter Massenspektrometrie werden krankheitsspezifische Veränderungen von Polypeptiden im Urinproteom detektiert und als relevant erkannte Polypeptide als diagnostische Biomarker eingesetzt. Wir berichten hier über die Ergebnisse von mehreren Studien an ca. 1000 Patienten und Kontrollpersonen.

Die erhaltenen Daten zeigen, dass Prostata- und Urothelkarzinome mit Hilfe von spezifischen Polypeptidmustern im Urin erkannt werden können. Erste weiterführende Untersuchungen deuten zudem an, dass auch die Invasivität des Urothelkarzinoms mit Hilfe der Proteomanalyse abgeschätzt werden kann. Diese neue nicht-invasive Untersuchungsmethode von Urinproteinmustern könnte zu einer Verbesserung der bislang verfügbaren diagnostischen Methoden beitragen.

Abstract

We developed a proteomics-based technology for the non-invasive detection of urothelial and prostate carcinoma. Using capillary electrophoresis coupled to mass spectrometry, disease-specific changes in the urinary proteome were detected and subsequently relevant polypeptides were employed as disease-specific biomarkers. Here we report the results of various studies including approximately 1,000 patients with different diseases and healthy volunteers.

The results of these studies revealed that prostate and urothelial carcinoma can be detected by using disease-specific polypeptide patterns. Preliminary results also indicate that the tumour stage of an urothelial carcinoma can be estimated by this approach. In conclusion, this new and non-invasive application might help to improve the diagnostic methods already available.

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Danksagung

Wir danken Hr. PD Dr. med. Axel Semjonow für die kritische Durchsicht des vorliegenden Manuskriptes.

Interessenkonflikt

Dr. Wittke ist Mitarbeiter der Diapat GmbH. Dr. Schiffer ist Mitarbeiter der Mosaiques Diagnostics and Therapeutics AG (der Muttergesellschaft der Diapat GmbH).

Trotz des möglichen Interessenkonflikts ist der Beitrag unabhängig und produktneutral.

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Authors and Affiliations

  1. Diapat GmbH, Mellendorfer Straße 7–9, 30625, Hannover, Deutschland

    S. Wittke

  2. Mosaiques Diagnostics and Therapeutics AG, Hannover, Deutschland

    E. Schiffer

  3. Urologie Maximilianstraße, München, Deutschland

    H.W. Bauer

Authors
  1. S. Wittke
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  2. E. Schiffer
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  3. H.W. Bauer
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Corresponding author

Correspondence to S. Wittke.

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Wittke, S., Schiffer, E. & Bauer, H. Kapillarelektrophorese gekoppelte Massenspektrometrie zur Proteomanalyse. Urologe 46, 733–739 (2007). https://doi.org/10.1007/s00120-007-1302-0

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  • DOI: https://doi.org/10.1007/s00120-007-1302-0

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