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Thyroid peroxidase in endemic goiter tissue

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Abstract

Thyroid peroxidase (TPO) activity and TPO protein were analyzed in endemic goiter tissue under iodine deficiency and after iodine supplement. TPO was prepared from 9 endemic goiter tissues and 4 normal thyroid tissues by solubilizing enzyme with detergent. Four patients with endemic goiter received iodized oil injection 12 months before surgery. All patients had normal serum thyrotropin (TSH) and thyroid hormone levels before surgery. TPO activity was measured by iodinase assay and guaiacol assay. Endemic goiter TPO showed greater iodination activity than that of normal TPO (p<0.01). The guaiacol assay showed greater TPO activity in 6 of the 9 endemic goiter tissues than that of the normal tissue. Iodized oil treatment did not affect TPO activity or TPO proteins when compared with those in untreated endemic goiter tissues. TPO activity in endemic goiter tissue correlated with thyroid T4 5′-deiodinase activity and not with thyroid hormone content in thyroglobulin. Since thyroid T4 5′-deiodinase and TPO are under control of TSH, an increase in TPO activity in the presence of normal serum TSH may be explained by the increased sensitivity of endemic goiter tissue to TSH-one of the possible adaptation mechanisms of endemic goiter.

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References

  1. Hetzel BS. Iodine deficiency disorders (IDD) and their eradication. Lancet 2: 1126, 1983.

    Article  PubMed  CAS  Google Scholar 

  2. Hershman J.M., Due D.T., Sharp B., My L., Kent J.R., Binh L.N., Reed A.W., Puch L.D., Van Herle A.J., Thai N.G., Troung T.X., Van N.V., Sugawara M., Pekary A.E. Endemic goiter in Vietnam. J. Clin. Endocrinol. Metab. 57: 243, 1983.

    Article  PubMed  CAS  Google Scholar 

  3. Goyens P., Goldstein B, Nsombola B, Vis H., Dumont J.E. Selenium deficiency as a possible factor in the pathogenesis of mixoedematous endemic cretinism. Acta. Endocrinol. (Copenh). 114: 497, 1987.

    PubMed  CAS  Google Scholar 

  4. Maberly G.F., Eastman C.J., Corcoran J.M. Thyroid hormone response to thyrotropin releasing hormone stimulation in subjects from endemic goiter regions of Sarawaka, Malaysia. Aust. N. Zeal. J. Med. 9: 385, 1979.

    Article  CAS  Google Scholar 

  5. Medeiros-Neto G.A. Endemic goiter and endemic cretinism. In: DeGroot L.J., (Eds.), Endocrinology, ed.2. W.B. Saunders, Philadelphia 1989, p. 746.

    Google Scholar 

  6. Prettel E., Moneloa F., Salinas R, Karvano A., Guerra-Garcia R., Betila I. Prophylaxis and treatment of endemic goiter in Peru with iodidized oil injection. J. Clin. Endocrinol. Metab. 29: 1586, 1969.

    Article  Google Scholar 

  7. Maberly G.F., Corcoran J.M., Eastman C.J. The effect of iodized oil on goiter size, thyroid function and the development of the Jod Basedow chiminate phenomenon. Clin. Endocrinol. (Oxf.) 17: 253, 1982.

    Article  CAS  Google Scholar 

  8. Vanderpass J.B., Rivera-Vanderpass M.T., Bourdoux P., Luvivila K., Lagasse R., Perlmutter-Cremer N., Delange F., Lanoie L., Ermans A.M., Thilly C.H. Reversibility of severe hypothyroidism with supplementary iodine in patients with endemic cretinism. N.Engl. J. Med. 315: 791, 1986.

    Article  Google Scholar 

  9. DeGroot L.J., Thompson J.E., Dunn A.D. Studies on an iodination enzyme from calf thyroid. Endocrinology 76: 632, 1965.

    Article  PubMed  CAS  Google Scholar 

  10. Taurog A. Thyroid peroxidase and thyroxine biosynthesis. Recent. Prog. Horm. Res. 26: 189, 1970.

    PubMed  CAS  Google Scholar 

  11. Bray G.A. Increased sensitivity of the thyroid in iodine-depleted rats to the goitrogenic effects of thyrotropin. J. Clin. Invest. 47: 1640, 1968.

    Article  PubMed Central  PubMed  CAS  Google Scholar 

  12. Halmi N.S., Spirtos B.N. Analysis of the modifying effect of dietary iodine levels on the thyroidal response of hypophysectomized rats to thyrotropin. Endocrinology 56: 157, 1955.

    Article  PubMed  CAS  Google Scholar 

  13. Nagataki S., Hichimune H., Masuyama Y., Nakao K. Thyrotropin and thyroid peroxidase activity. Endocrinology 92: 363, 1975.

    Article  Google Scholar 

  14. Sugawara M., Trayer C.L., Kita T., Kuma K. Improved method of thyroid peroxidase extraction from the human thyroid gland. Clin. Chimica Acta 151: 17, 1985.

    Article  CAS  Google Scholar 

  15. Sugawara M. Coupling of iodotyrosines catalyzed by human thyroid peroxidase. J. Clin. Endocrinol. Metab. 60: 1069, 1985.

    Article  PubMed  CAS  Google Scholar 

  16. Inoue K., Taurog A. Digestion of 131l-labeled thyroid tissue with maximum recovery of 131l-iodothyronines. Endocrinology 81: 319, 1967.

    Article  PubMed  CAS  Google Scholar 

  17. Hosoya T., Kondo Y., Ui N. Peroxidase activity in thyroid gland and partial purification of the enzyme. J. Biochem. 52: 180, 1962.

    PubMed  CAS  Google Scholar 

  18. Burnette W.N. “Western Blotting”; electrophoretic transfer of proteins from sodium dodecylsulfate polyaccrylamide gels to unmodified nitrocellulose and radiographic detection with antibody and radioiodinated protein A. Anal. Biochem. 112: 195, 1981.

    Article  PubMed  CAS  Google Scholar 

  19. Czamock B, Ruf J., Ferrard M., Carayon P, Lissitzky S. Purification of the human thyroid peroxidase and its identification as microsomal antigen involved in autoimmune thyroid diseases. FEBS Lett. 190: 147, 1985.

    Article  Google Scholar 

  20. Portman L., Fitch F.W., Hauran W., Hamada N., Franklin W.A., DeGroot L.J. Characterization of the thyroid microsomal antibodies and its relationship to thyroid peroxidase, using monoclonal antibodies. J.Clin. Invest. 81: 1217, 1988.

    Article  Google Scholar 

  21. Sugarawa M, Lau R., Wasser H.L., Nelson A.M., Kuma K, Hershman J.M. Thyroid T4 5′-deiodinase activity in normal and abnormal human thyroid glands. Metabolism 33: 332, 1984.

    Article  Google Scholar 

  22. Lowry O.H., Rosenbrough N.J., Far A.L, Randall R.J. Protein mesurement with the Folin phenol reagent. J. Biol. Chem. 193: 265, 1951.

    PubMed  CAS  Google Scholar 

  23. Hill B.J., Whatley S. A simple microassay of DNA. FEBS Lett. 1: 20, 1975.

    Article  Google Scholar 

  24. Portmann L., Fitch F.W., Havran W., Hamada N., Franklin W.A., DeGroot L.J. Characterization of the thyroid microsomal antigen, and its relationship to thyroid peroxidase, using monoclonal antibodies. J. Clin. Invest. 81: 1217, 1988.

    Article  PubMed Central  PubMed  CAS  Google Scholar 

  25. Wu S.Y. Thyrotropin-mediated induction of thyroidal iodothyronine monedeiodinase in the dog. Endocrinology 112: 417, 1983.

    Article  PubMed  CAS  Google Scholar 

  26. Magnusson R.R., Rapoport B. Modulation of differentiated function in cultured thyroid cell: thyrotropin control of thyroid peroxidase. Endocrinology 116: 1493, 1985.

    Article  PubMed  CAS  Google Scholar 

  27. Ishii H., Inada M, Tanada K., Mashio Y., Natio K., Nishikawa M., Matsuzuka M., Kuma K., Imura H. Triiodothyronine generation from thyroxine in human thyroid: enhanced conversion of Graves’ tissue. J. Clin. Endocrinol. Metab. 57: 500, 1983.

    Article  PubMed  CAS  Google Scholar 

  28. Medeiros-Neto G.A., Halpern A., Cozzi Z.S., Lima N., Kohn L.D. Thyroid growth immunoglobulins in large multinodular endemic goiters: effect of iodized oil. J. Clin. Endocrinol. Metab. 63: 644, 1986.

    Article  PubMed  CAS  Google Scholar 

  29. Hetzel B.S., Thilly C.H., Fierro-Benitez R., Prettel E.A., Buttfield I.H., Stanburry J.B. Iodized oil in the prevention of endemic goiter and endemic cretinism. In: Stanbury J.B., Hetzel B.S. (Eds.). Endemic goiter and endemic cretinism. John Wiley and Son, New York, 1980, p. 513.

    Google Scholar 

  30. Takamatsu J. Sugawara M., Kuma K., Kobayashi A., Matsuzuka F., Mozai T., Hershman J.M. Ratio of serum triiodothyronine to thyroxine and prognosis of tiirodothytonine-predominant Graves’ disease. Ann. Int. Med. 100: 372, 1984.

    Article  PubMed  CAS  Google Scholar 

  31. Boyages S.C., Halpern J.P., Maberly G.F., Collins J., Jupp J, Eastman C.J., Chen-En J, Yuhai G., Lin Z. Supplementary iodine fails to reverse hypothyroidism in a adolescents and adults with endemic cretinism. J. Clin. Endocrinol. Metab. 70: 336, 1990.

    Article  PubMed  CAS  Google Scholar 

  32. Inoue K., Taurog A. Acute and chronic effects of iodide on thyroid radioiodine metabolism in iodine-deficient rats. Endocrinology 83: 279, 1968.

    Article  PubMed  CAS  Google Scholar 

  33. Inoue K, Taurog A. Sedimentation pattern of soluble protein from thyroids of iodine deficient rats. Acute effects of iodine. Endocrinology 83: 816, 1968.

    Article  PubMed  CAS  Google Scholar 

  34. Ermans A.M., Dumont J.E, Bastenie P.A. Thyroid function in a goiter endemic: I. Impairment of hormone synthesis and secretion in the goitrous gland. J. Clin. Endocrinol. Metab. 23: 539, 1963.

    Article  CAS  Google Scholar 

  35. DeGroot L.J., Larsen P.R., Refetoff S., Stanbury J.B. The thyroid and its diseases, ed. 5. John Wiley & Sons, New York, 1984. p. 642.

    Google Scholar 

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Authors and Affiliations

  1. Endocrine Division, Medical and Research Services, Wadsworth Veterans Administration Hospital and Department of Medicine, UCLA School of Medicine, Los Angeles, Wilshire and Sawtelle Blvds, Los Angeles, California, 90073, USA

    M. Sugawara, C. N. Summer & S. Murakami

  2. Kuma Hospital, Kobe, Japan

    A. Kobayashi & K. Kuma

  3. Thyroid Laboratory, University of São Paulo, School of Medicine, Brazil

    G. A. Medeiros-Neto

Authors
  1. M. Sugawara
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  2. C. N. Summer
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  3. A. Kobayashi
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  4. S. Murakami
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  5. K. Kuma
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  6. G. A. Medeiros-Neto
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This study was supported by Veterans Administration Medical Research Funds.

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Sugawara, M., Summer, C.N., Kobayashi, A. et al. Thyroid peroxidase in endemic goiter tissue. J Endocrinol Invest 13, 893–899 (1990). https://doi.org/10.1007/BF03349648

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  • DOI: https://doi.org/10.1007/BF03349648

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