Abstract
We present data demonstrating that the strain DBVPG 3827 does not belong toC. stellata. From the results of physiological analysis, electrophoretic karyotyping, the PCR-RFLP of the ITS1-5.8S-ITS2 region and the nucleotide sequence of the D1/D2 domain of the 26S rDNA it can be concluded that DBVPG 3827 is a strain ofStarmerella bombicola. This finding, and the recent observation thatC. stellata can easily be confused withC. zemplinina in tests of conventional taxonomy, urges a critical revision of the enological role(s) attributed by researchers toC. stellata.
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References
Antunovics Y., Csoma H., Sipiczki M.: Molecular and genetic analysis of the yeast flora of botrytiyed Tokaj wines.Bulletin de l’O.I.V. 76, 380–397 (2003).
Capriotti A., Rainieri L.: Yeasts in greenhouse environment. I. From soils, flowers and animals.Arch.Microbiol. 48, 325–331 (1964).
Ciani M., Ferraro L.: Enhanced glycerol content in wine made with immobilizedCandida stellata cells.Appl.Environ.Microbiol. 62, 128–132 (1996).
Ciani M., Ferraro L.: Combined use of immobilizedCandida stellata cells andSaccharomyces cerevisiae to improve the quality of wines.J.Appl.Microbiol. 85, 247–254 (1998).
Ciani M., MacCarelli F.: Enological properties of non-Saccharomyces yeasts associated with winemaking.World J.Microbiol.Biotechnol. 14, 199–203 (1998).
Ciani M., Picciotti G.: The growth kinetics and fermentation behavior of some non-Saccharomyces yeasts associated with wine making.Biotechnol.Lett. 17, 1247–1250 (1995).
Ciani M., Ferraro L., Fatichenti F.: Influence of glycerol production on the aerobic and anaerobic growth of the wine yeastCandida stellata.Enzyme Microb.Technol. 27, 698–703 (2000).
Constanti M., Poblet M., Arola L., Mas A., Guillamon J.M.: Analysis of yeast populations during alcoholic fermentation in a newly established winery.Am.J.Enol.Viticult. 48, 339–344 (1997).
Ferraro L., Fatichenti F., Ciani M.: Pilot scale vinification process using immobilizedCandida stellata cells andSaccharomyces cerevisiae.Proc.Biochem. 35, 1125–1129 (2000).
Fleet G.H., Heard G.M.: Yeasts — growth during fermentation, pp. 27–54 in G.H. Fleet (Ed.):Wine Microbiology and Biotechnology. Harwood Academic Publishers, Chur (Switzerland) 1993.
Granchi L., Bosco M., Messini A., Vincenzini M.: Rapid detection and quantification of yeast species during spontaneous wine fermentation by PCR-RFLP analysis of the rDNA ITS region.J.Appl.Microbiol. 87, 949–956 (1999).
Guillamon J.M., Sabate J., Barrio E., Cano J., Querol A.: Rapid identification of wine yeast species based on RFLP analysis of the ribosomal internal transcribed spacer (ITS) region.Arch.Microbiol. 169, 387–392 (1998).
Kurtzman C.P., Robnett C.J.: Identification and phylogeny of ascomycetous yeasts from analysis of nuclear large subunit (26S) ribosomal DNA partial sequences.Antonie van Leeuwenhoek 73, 331–371 (1998).
de Llanos Frutos R., Fernandez-Espinar M.T., Querol A.: Identification of species of the genusCandida by analysis of the 5.8S rRNA gene and the two ribosomal internal transcribed spacers.Antonie van Leeuwenhoek 85, 175–185 (2004).
Mills D.A., Johannsen E.A., Cocolin L.: Yeast diversity and persistence inBotrytis-affected wine fermentations.Appl.Environ.Microbiol. 68, 4884–4893 (2002).
Mora J., Mulet A.: Effects of some treatments of grape juice on the population and growth of yeast species during fermentation.Am.J.Enol.Viticult. 42, 133–136 (1991).
Soden A., Francis I.L., Oakey H., Henschke P.A.: Effects of cofermentation withCandida stellata andSaccharomyces cerevisae on the aroma and composition of Chardonnay wine.Austral.J.Grape Wine Res. 6, 21–30 (2000).
Sipiczki M.:Candida zemplinina sp.nov., an osmotolerant and psychrotolerant yeast that ferments sweet botrytized wines.Internat.J.Syst.Evol.Microbiol. 53, 339–343 (2003).
Sipiczki M.: Species identification and comparative molecular and physiological analysis ofCandida zemplinina andCandida stellata.J.Basic Microbiol. 44, 471–479 (2004).
Sipiczki M., Miklos I., Leveleki L., Antunovics Z.: Genetic and chromosomal stability of wine yeasts.Meth.Biotechnol. 14, 273–281 (2001).
Van der Walt J.P., Yarrow D.: Methods for the isolation, maintenance, classification and identification of yeasts, pp. 45–104 in N.J.W. Kreger-van Rij (Ed.):The Yeasts: a Taxonomic Study. Elsevier, Amsterdam 1984.
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This work was supported by the grant NKFP-4/0007/2002 of theHungarian Ministry of Education.
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Šipiczki, M., Ciani, M. & Csoma, H. Taxonomic reclassification ofCandida stellata DBVPG 3827. Folia Microbiol 50, 494–498 (2005). https://doi.org/10.1007/BF02931436
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DOI: https://doi.org/10.1007/BF02931436