Abstract
The transport of α-methyl-D-glucoside and two aminoacids, L-phenylalanine and L-leucine by a temperature sensitive fatty acid requiring mutant ofSalmonella typhimurium was studied under conditions of supplementation withcis or trans-unsaturated fatty acids. The results of such experiments definitely establish a relationship between the fatty acids composition of the membrane and the transport property of the cells. Cells grown in the presence of trans-unsaturated fatty acids cannot transport so efficiently as compared to the cis-unsaturated fatty acid-grown cells except linolelaidic acid, atrans-trans-unsaturated fatty acid. Protein: phospholipid ratio of the membrane also varies significantly under such conditions. The affinity of L-phenylalanine transport carrier for the substrate changes remarkably in cells grown in the presence of differentcis or trans-unsaturated fatty acids and indicate the possible role of membrane lipids in membrane assembly as well as regulation of the activity of L-phenylalanine transport system.
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Abbreviations
- α-MG:
-
α-Methyl-D-glucoside
- MM:
-
minimal medium
- DPH:
-
l, 6-diphenyl-l, 3, 5, hextriene
- ANS:
-
1, 8-anilino naphthalene sulphonic acid
References
Bligh, E. G. and Dyer, W. J. (1959)Can. J. Biochem. Physiol.,37, 911.
Braford, M. M. (1976)Anal. Biochem.,72, 248.
Chakravorty, M. (1970)J. Virol.,5, 541.
Chen, P. S., Toribara, T. Y. and Warner, H. (1956)Anal. Biochem.,28, 1756.
Cronan, J. E. Jr. (1978)Annu. Rev. Biochem.,47, 163.
Deb, J. K., Biswas, S. K., Chakrabarti, P. and Chakravorty, M. (1986)J. Biosci.,10, 335.
Endo, A. and Rothfield, L. I. (1969)Biochemistry,8, 3508.
Esfahani, M., Rudkin, B. B., Cutler, C. J. and Waldron, P. E. (1977)J. Biol. Chem.,252, 3194.
Higashi, Y. and Strominger, J. L. (1970)J. Biol. Chem.,245, 3691.
Hofee, P., Engelsberg, E. and Lamy, F. (1964)Biochim. Biophys. Acta,79, 337.
Hong, J. S. and Ames, B. N. (1971)Proc. Natl. Acad. Sci. USA,68, 3158.
Jakovcic, S., Getz, G. S., Rabinowitz, M., Jakob, H. and Swift, S. (1971)J. Cell. Biol.,48, 490.
Kundig, W. andRoseman, S. (1975)J. Biol. Chem.,246, 1407.
Khandekar, P. S., Burma, D. P., Taneja, S. and Chakravorty, M. (1975)J. Virol.,16, 101.
Lowry, O. H., Rosebrough, N. J., Farr, A. L. and Randall, R. J. (1951)J. Biol. Chem.,193, 265.
Mandal, S. B., Sen, P. C, Chakrabarti, P. and Sen, K.(1978)Can. J. Microbiol.,24, 586.
Milner, L. S. and Kaback, H. R. (1970)Proc. Natl. Acad. Sci. USA,66, 1008.
Ovarth, P., Schairer, H. M. and Stoffel, W. (1970)Proc. Natl. Acad. Sci. USA,67, 606.
Schneider, E. G. and Kennedy, E. P. (1973)J. Biol. Chem.,248, 3739.
Silbert, D. F. (1975)Annu. Rev. Biochem.,44, 315.
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Deb, J.K., Goyal, R. & Chakravorty, M. Effect ofcis andtrans unsaturated fatty acids on the transport properties ofSalmonella typhimurium . J. Biosci. 11, 145–153 (1987). https://doi.org/10.1007/BF02704665
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DOI: https://doi.org/10.1007/BF02704665