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Regulation of glutamine synthetase in L cells by cortisol andl-glutamine

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Summary

The glutamine synthetase system of strain L mouse cells is regulated by at least two natural substances: glutamine and a glucocorticoid hormone. In a chemically defined cell culture system, cortisol or dexamethasone promotes intracellular accumulation of the synthetase by a process which depends upon genetic transcription. The accumulation is most rapid if the hormone is added to a glutamine deficient culture medium. High concentrations of glutamine promote rapid disappearance of the enzyme by a mechanism which depends on unimpaired protein synthesis. Ways in which the two regulatory substances collaborate to influence the synthetase level and other growth-related processes of L cells in culture are discussed.

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Author notes

  1. Paul R. Barnes

    Present address: Department of Physiology and Behavioural Biology, California State University, San Francisco, 94132, San Francisco, California

Authors and Affiliations

  1. Department of Biochemistry, University of Kansas, 66044, Lawrence, Kansas

    Paul R. Barnes, Robert T. Hersh & Paul A. Kitos

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  1. Paul R. Barnes
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  2. Robert T. Hersh
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Additional information

This research was supported in part by Research Grant GB 13924 from the National Science Foundation, by the University of Kansas General Research Fund, and by NIH Biomedical Sciences Support Grant FR 07037.

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Barnes, P.R., Hersh, R.T. & Kitos, P.A. Regulation of glutamine synthetase in L cells by cortisol andl-glutamine. In Vitro 9, 230–238 (1974). https://doi.org/10.1007/BF02616068

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