Skip to main content
SpringerLink
Log in

High-performance liquid chromatography with light-scattering detection and desorption chemical-ionization tandem mass spectrometry of milk fat triacylglycerols

  • Article
  • Published:
Lipids

Abstract

The utility of reverse-phase high-performance liquid chromatography (HPLC), desorption chemical-ionization mass spectrometry (DCI-MS) and tandem mass spectrometry (MS/MS) for the characterization of triacylglycerols in complex mixtures has been further explored. Triacylglycerols of anhydrous bovine milk fat were separated by using two reversephase C18 HPLC columns, and eluents were monitored with an evaporative light-scattering detector. Fifty-eight fractions were resolved and analyzed by positive ion isobutane DCI-MS. The formation of protonated molecules and of major fragments corresponding to the random loss of any one of the constituent fatty acids readily identified acyl carbon numbers and the number of double bonds within each fatty acid. MS/MS was only required when the original mass spectra indicated the presence of more than one triacylglycerol or of impurities in a fraction. Protonated molecules produced by DCI were fragmented using high energy collisional activation, and the resulting ions were detected by MS/MS. Odd-chain triacylglycerols were also readily distinguished using this methodology. The positive ion DCI and MS/MS techniques described here demonstrate the usefulness of this approach for the characterization of triacylglycerols in complex mixtures.

This is a preview of subscription content, log in via an institution to check access.

Access this article

Log in via an institution

Price excludes VAT (USA)
Tax calculation will be finalised during checkout.

Instant access to the full article PDF.

Institutional subscriptions

Similar content being viewed by others

Abbreviations

B/E:

magnetic field strength/electrostatic analyzer voltage

CI:

chemical ionization

DCI:

desorption chemical ionization

ECN:

equivalent carbon number

EI:

electron impact

ELSD:

evaporative light-scattering detection

GLC:

gas-liquid chromatography

HPLC:

high-performance liquid chromatography

MS:

mass spectrometry

MS/MS:

tandem mass spectrometry

PCN:

propionitrile

References

  1. Christie, W.W. (1987) inRecent Advances in Chemistry and Technology of Fats and Oils (Hamilton, R.J. and Bhati, A., eds.) pp. 57–78, Elsevier Science Publishing Co., New York.

    Google Scholar 

  2. Christie, W.W. (1981) inLipid Metabolism in Ruminant Animals (Christie, W.W., ed.) pp. 93–191, Pergamon Press, New York.

    Google Scholar 

  3. Myher, J.J. and Kuksis, A. (1982)Can. J. Biochem. 60, 638–650.

    Article  PubMed  CAS  Google Scholar 

  4. Christie, W.W. (1983)Developments in Dairy Chemistry 2. Lipids, pp. 1–35, Applied Science Publishers, New York.

    Google Scholar 

  5. Patton, S., and Jenson, R.G. (1975)Prog. Chem. Fats Other Lipids 14, 162–277.

    Article  Google Scholar 

  6. Bhati, A. (1987) inRecent Advances in Chemistry and Technology of Fats and Oils (Hamilton, R.J., and Bhati, A., eds.) pp. 13–30, Elsevier Science Publishing Co., New York.

    Google Scholar 

  7. Geeraert, E., and Sandra, P. (1987)J. Am. Oil Chem. Soc. 64, 100–105.

    CAS  Google Scholar 

  8. Murata, T., and Takahashi, S. (1977)Anal. Chem. 49, 728–731.

    Article  PubMed  CAS  Google Scholar 

  9. Rezanka, T., and Mares, P. (1991)J. Chromatogr. 542, 145–159.

    Article  CAS  Google Scholar 

  10. Robinson, J.L., and Macrae, R. (1984)J. Chromatogr. 303, 386–390.

    Article  CAS  Google Scholar 

  11. Deffense, E. (1984)Rev Franc. Corps Gras 31, 123–129.

    CAS  Google Scholar 

  12. Dotson, K.D., Jerrell, J.P., Picciano, M.F., and Perkins, E.G. (1992)Lipids 27, 933–939.

    PubMed  CAS  Google Scholar 

  13. Schulte, V.E. (1981)Fette Seifen Anstrichm. 83, 289–291.

    Article  CAS  Google Scholar 

  14. Frede, E., and Thilele, H. (1987)J. Am. Oil Chem. Soc. 64, 521–528.

    CAS  Google Scholar 

  15. Nikolova-Damyanova, B., Christie, W.W., and Herslöf, B. (1990)J. Am. Oil Chem. Soc. 67, 503–507.

    CAS  Google Scholar 

  16. Phillips, F.C., Erdahl, W.L., Nadenicek, J.D., Nutter, L.J., and Schmit, J.A. (1984)Lipids 19, 142–150.

    Article  CAS  Google Scholar 

  17. Maniongui, C., Gresti, J., Bugaut, M., Gauthier, S., and Bezard, J. (1991)J. Chromatogr. 543, 81–103.

    Article  PubMed  CAS  Google Scholar 

  18. Stolyhowo, A., Colin, H., Martin, M., and Guiochon, G. (1984)J. Chromatogr. 288, 253–275.

    Article  Google Scholar 

  19. Stolyhwo, A., Colin, H., and Guiochon, G. (1985)Anal. Chem. 57, 1342–1354.

    Article  PubMed  CAS  Google Scholar 

  20. Robinson, J.L., Tsimidou, M., and Macrae, R. (1985)J. Chromatogr. 324, 35–51.

    Article  PubMed  CAS  Google Scholar 

  21. Herslöf, B., and Kindmark, G. (1985)Lipids 20, 783–790.

    Article  Google Scholar 

  22. Currie, G.J., and Kallio, H. (1993)Lipids 28, 217–222.

    PubMed  CAS  Google Scholar 

  23. Kallio, H., and Currie, G.J. (1993)Lipids 28, 207–215.

    CAS  Google Scholar 

  24. Murata, T. (1977)Anal. Chem. 49, 2209–2213.

    Article  PubMed  CAS  Google Scholar 

  25. Kuksis, A., Myher, J.J. and Marai, L. (1985)J. Am. Oil Chem. Soc. 62, 762–767.

    Article  CAS  Google Scholar 

  26. Kuksis, A., Myher, J.J., and Marai, L. (1984)J. Am. Oil Chem. Soc. 61, 1582–1589.

    CAS  Google Scholar 

  27. Weintraub, S.T. (1990) inMass Spectrometry of Biological Materials (McEwen, C.N., and Larsen, B.S., eds.) pp. 257–286, Marcel Dekker, New York.

    Google Scholar 

  28. van Breemen, R.B. (1992) inMass Spectrometry in the Biological Sciences: A Tutorial (Gross, M.L., ed.) pp. 443–451, Kluwer Academic Publishers, Dordrecht.

    Google Scholar 

  29. Adams, J. (1990)Mass Spectrom. Rev. 9, 141–186.

    Article  CAS  Google Scholar 

  30. Laakso, P.H., Nurmela, K.V.V., and Homer, D.R. (1992)J. Agr. Food Chem. 40, 2472–2482.

    Article  CAS  Google Scholar 

Download references

Author information

Author notes

  1. George A. Spanos

    Present address: Applied Analytical Industries, 1206 North 23rd St., 28405, Wilmington, NC

  2. Richard B. van Breemen

    Present address: Department of Medicinal Chemistry and Pharmacognosy, University of Illinois at Chicago, 833 South Wood St., 606127231, Chicago, IL

Authors and Affiliations

  1. Southeast Dairy Foods Research Center, Department of Food Science, North Carolina State University, Box 7624, 276957624, Raleigh, NC

    George A. Spanos & Steven J. Schwartz

  2. Department of Chemistry, North Carolina Sate University, 27695, Raleigh, North Carolina

    Richard B. van Breemen & Chien-Hua Huang

Authors
  1. George A. Spanos
    View author publications

    You can also search for this author in PubMed Google Scholar

  2. Steven J. Schwartz
    View author publications

    You can also search for this author in PubMed Google Scholar

  3. Richard B. van Breemen
    View author publications

    You can also search for this author in PubMed Google Scholar

  4. Chien-Hua Huang
    View author publications

    You can also search for this author in PubMed Google Scholar

About this article

Cite this article

Spanos, G.A., Schwartz, S.J., van Breemen, R.B. et al. High-performance liquid chromatography with light-scattering detection and desorption chemical-ionization tandem mass spectrometry of milk fat triacylglycerols. Lipids 30, 85–90 (1995). https://doi.org/10.1007/BF02537046

Download citation

  • Received:

  • Revised:

  • Accepted:

  • Issue Date:

  • DOI: https://doi.org/10.1007/BF02537046

Keywords

Search

Navigation