Abstract
Microtiter plate methods were developed for the enzymatic determination of serum total cholesterol (TC), high density lipoprotein cholesterol (HDL-C) and triglyceride (TG), and for the turbidometric determination of apolipoproteins. The micromethods resulted in accurate, precise values that were in good agreement with the conventional spectrophotometric assays. The coefficient of variation for TC determinations was 4.5% or less and bias was 5% or less. The lipid micromethod assays are sensitive to 10 mg/dL or less, and the apolipoprotein assay to 1 mg/dL. Less than 100 μL of serum suffices for TC, TG and apoprotein assays; HDL-C requires an additional 100 μL of serum. Advantages of the micromethods include reductions in assay time and in the amount of reagents required.
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Abbreviations
- apo:
-
apolipoprotein
- CV:
-
coefficient of variation
- ELISA:
-
enzyme linked immunosorbent assay
- HDL-C:
-
high density lipoprotein cholesterol
- NBS:
-
National Bureau of Standards
- NCEP:
-
National Cholesterol Education Program
- PBS:
-
phosphate-buffered saline; std, standard
- TC:
-
total cholesterol
- TG:
-
triglyceride
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Shireman, R.B., Durieux, J. Microplate methods for determination of serum cholesterol, high density lipoprotein cholesterol, triglyceride and apolipoproteins. Lipids 28, 151–155 (1993). https://doi.org/10.1007/BF02535780
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DOI: https://doi.org/10.1007/BF02535780