Skip to main content
SpringerLink
Log in

Heterogeneity of molecular weight and apolipoproteins in low density lipoproteins of healthy human males

  • Communication
  • Published:
Lipids

Abstract

The molecular weights of five low density lipoprotein (LDL) subfractions from four normal healthy males were determined by analytic ultracentrifuge sedimentation equilibria. Protein content of each subfraction was determined by elemental CHN analysis, and weights of apoprotein peptides were calculated. Molecular weights in subfractions of increasing density were 2.92±0.26, 2.94±0.12, 2.68±0.09, 2.68±0,28 and 2.23 ±0,22 million Da, and protein weight percentages were 21.05, 21.04, 22.05, 23.10 and 29.10, in subfractions, 1,2,3,4 and 5, respectively. Total mean apoprotein weights for respective subfractions were 614±53,621±45, 588±9,637±83 and 645 ±62 KDa. In addition to a single apoprotein B-100 (apo B-100) peptide with a mean carbohydrate content of 7.1% and a molecular weight of 550 KDa per LDL particle, there may be one or more apoprotein E peptides of 34 KDa and/or apoprotein C-III of 9 KDa. In addition, subfractions 4 and 5 may contain 3–7% apolipoprotein (a). There is considerable heterogeneity among LDL subfractions as well as within the same fraction from different individuals. This heterogeneity may relate to differences in origin, metabolism and/or atherogenicity as a result of their content of apoproteins other than apo B-100.

This is a preview of subscription content, log in via an institution to check access.

Access this article

Log in via an institution

Price excludes VAT (USA)
Tax calculation will be finalised during checkout.

Instant access to the full article PDF.

Institutional subscriptions

Similar content being viewed by others

Abbreviations

apo B-100:

apolipoprotein B-100

apo LP:

apolipoprotein

EDTA:

ethylenediaminetetraacetic acid dipotassium salt

IDL:

intermediate density lipoproteins

LDL:

low density lipoproteins

VLDL:

very low density lipoproteins

References

  1. Chen, S., Yang, C., Chen, P., Setzer, D., Tanimura, M., Li, W., Gotto, A.M., and Chan, L. (1986)J. Biol. Chem. 261, 12918–12921.

    PubMed  CAS  Google Scholar 

  2. Knott, T.J., Pease, R.J., Powell, L.M., Wallis, S.C., Rall, S.C., Innerarity, T.L., Blackhart, B., Taylor, W.H., Marcel, Y., Milne, R., Johnson, D., Fuller, M., Lusis, A.J., McCarthy, B.J., Mahley, R.W., Levy-Wilson, B., and Scott, J. (1986)Nature 323, 734–738.

    Article  PubMed  CAS  Google Scholar 

  3. Yang, C., Chen, S., Gianturco, S.H., Bradley, W.A., Sparrow, J.T., Tanimura, M., Li, W., Sparrow, D.A., DeLoof, H., Rosseneu, M., Lee, F., Gu, Z., Gotto, A.M., and Chan, L. (1986)Nature 323, 738–742.

    Article  PubMed  CAS  Google Scholar 

  4. Law, S.W., Grant, S.M., Higuchi, K., Hospattankar, A., Lackner, K., Lee, N., and Brewer, H.B. (1986)Proc. Natl. Acad. Sci. USA 83, 8142–8146.

    Article  PubMed  CAS  Google Scholar 

  5. La Belle, M., and Krauss, R.M. (1990)J. Lipid Res. 31, 1577–1588.

    PubMed  Google Scholar 

  6. Campos, E., and McConathy, W.J. (1986)Arch. Biochem. Biophys. 249, 455–463.

    Article  PubMed  CAS  Google Scholar 

  7. Lee, D.M. and Alaupovic, P. (1986)Biochim. Biophys. Acta 879, 126–133.

    PubMed  CAS  Google Scholar 

  8. Chapman, J.M., Laplaud, P.M., Luc, G., Forgez, P., Bruckert, E., Goulinet, S., and Lagrange, D. (1988)J. Lipid Res. 29, 442–458.

    PubMed  CAS  Google Scholar 

  9. Shen, M.M.S., Krauss, R.M., Lindgren, F.T., and Forte, T.M. (1981)J. Lipid Res. 22, 236–244.

    PubMed  CAS  Google Scholar 

  10. Kahlon, T.S., Glines, L.A., and Lindgren, F.T. (1986)Methods Enzymol. 129, 26–45.

    Article  PubMed  CAS  Google Scholar 

  11. Kahlon, T.S., Adamson, G.L., Glines, L.A., Orr, J.R., and Lindgren, F.T. (1986)Lipids 21, 235–238.

    Article  PubMed  CAS  Google Scholar 

  12. Schachman, H.K. (1957)Methods Enzymol. 4, 1–103.

    Google Scholar 

  13. Kahlon, T.S., Adamson, G.L., Shen, M.M.S., and Lindgren, F.T. (1982)Lipids 17, 323–330.

    PubMed  CAS  Google Scholar 

  14. Wiklund, O., Dyer, C.A., Tsao, B.P., and Curtiss, L.K. (1985)J. Biol. Chem. 260, 10956–10960.

    PubMed  CAS  Google Scholar 

  15. Young, S.G., Northey, S.T., and McCarthy, B.J. (1988)Science 241, 591–593.

    Article  PubMed  CAS  Google Scholar 

  16. Krul, E.S., Kinoshita, M., Talmud, P., Humphries, S.E., Turner, S., Goldberg, A.C., Cook, K., Boerwinkel, E., and Schonfeld, G. (1989)Arteriosclerosis 9, 856–868.

    PubMed  CAS  Google Scholar 

  17. Wagner, R.D., Krul, E.S., Tang, J., Parhofer, K.G., Garlock, K., Talmud, P., and Schonfeld, G. (1991)J. Lipid Res. 32, 1001–1011.

    PubMed  CAS  Google Scholar 

  18. Lindgren, F.T., Adamson, G.L., Shore, V.G., Nelson, G.J., and Schmidt, P.C. (1991)Lipids 26, 97–101.

    PubMed  CAS  Google Scholar 

Download references

Author information

Authors and Affiliations

  1. Lawrence Livermore National Laboratory, 94550, Livermore, California

    Virgie G. Shore

  2. Donner Laboratory, University of California, 94720, Berkeley, California

    Frank T. Lindgren

  3. Western Regional Research Center, USDA-ARS, 800 Buchanan Street, 94710, Albany, CA

    Talwinder S. Kahlon

Authors
  1. Talwinder S. Kahlon
    View author publications

    You can also search for this author in PubMed Google Scholar

  2. Virgie G. Shore
    View author publications

    You can also search for this author in PubMed Google Scholar

  3. Frank T. Lindgren
    View author publications

    You can also search for this author in PubMed Google Scholar

About this article

Cite this article

Kahlon, T.S., Shore, V.G. & Lindgren, F.T. Heterogeneity of molecular weight and apolipoproteins in low density lipoproteins of healthy human males. Lipids 27, 1055–1057 (1992). https://doi.org/10.1007/BF02535588

Download citation

  • Received:

  • Revised:

  • Accepted:

  • Issue Date:

  • DOI: https://doi.org/10.1007/BF02535588

Keywords

Search

Navigation