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Incorporation of deuterium-labeledcis- andtrans-9-octadecenoic acids in humans: Plasma, erythrocyte, and platelet phospholipids

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Lipids

Abstract

The objective of this study was to follow the uptake and distribution of oleic and elaidic acids into human erythrocytes, platelets, and plasma phospholipids. The use of dual and triple labeling methodology permitted a precise comparison of elaidic and oleic acid utilization. Elaidic acid (EI) was selectively concentrated in all the plasma phospholipids except for lysophosphatidylcholine. Three times more elaidic than oleic acid (Ol) accumulated in the 1-acyl position of phosphatidylcholine, as determined by hydrolysis with phospholipase A2. Rapid incorporation and removal of elaidate were observed for all samples. These results support the concept that enzymes responsible for acylation of phospholipids are sensitive to double bond configuration and the physical properties of the fatty acid moieties. Labeled fatty acid levels in red cell and platelet phospholipids were much lower than for plasma phospholipids, indicating a relatively slow rate for the in vivo incorporation of fatty acids into blood cell membrane phospholipids. No isotope effect was found when oleic acid labeled with deuterium on the double bond was used.

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Authors and Affiliations

  1. Northern Regional Research Center, Agricultural Research, Science and Education Administration, U.S. Department of Agriculture, 61604, Peoria, IL

    E. A. Emken, W. K. Rohwedder, H. J. Dutton, W. J. Dejarlais & R. O. Adlof

  2. Georgetown Medical School, 20005, Washington, DC

    J. F. Mackin

  3. Lipid Nutrition Laboratory, Nutrition Institute, Agricultural Research, Science and Education Administration, U.S. Department of Agriculture, 20705, Beltsville, MD

    R. M. Dougherty

  4. Marketing, Nutrition and Engineering Sciences, Agricultural Research, Science and Education Administration, U.S. Department of Agriculture, 20250, Washington, DC

    J. M. Iacono

Authors
  1. E. A. Emken
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  2. W. K. Rohwedder
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  3. H. J. Dutton
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  4. W. J. Dejarlais
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  5. R. O. Adlof
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  6. J. F. Mackin
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  7. R. M. Dougherty
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  8. J. M. Iacono
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The mention of firm names or trade products does not imply that they are endorsed or recommended by the U.S. Department of Agriculture over other firms or similar products not mentioned.

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Emken, E.A., Rohwedder, W.K., Dutton, H.J. et al. Incorporation of deuterium-labeledcis- andtrans-9-octadecenoic acids in humans: Plasma, erythrocyte, and platelet phospholipids. Lipids 14, 547–554 (1979). https://doi.org/10.1007/BF02533530

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  • DOI: https://doi.org/10.1007/BF02533530

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