Abstract
We have performed the immunohistochemical analysis for PTHrP expression in normal and 60 lung, 108 uterine, 28 breast, 42 renal, 22 ovarian and 33 gastric neoplastic tissues. Normal epithelial tissues, such as keratinocytes, renal tubules and mammary ductal cells showed positive immunoreactivity. In lung cancer, squamous (94%) and small cell (89%) carcinoma were positive regardless to the association with hypercalcemia and adenocarcinoma was negative. In uterine cervical neoplasia, 96% of squamous cell carcinoma were positive and higher immunoreactivity was observed in the cases with keratinization or prominent stromal cell interaction. 57% of brea st cancer examined were positive and immunoreactivity was most intensely observed in papillotubular and scirrhous types. Among the metastatic sites of breast cancer, skeletal lesion showed significantly higher expression of PTHrP than other sites. PTHrP was detected in 95% of renal cell carcinoma but the incidence of hypercalcemia was only 14%. In ovarian neoplasia, serous cystadenocarcinoma and clear cell carcinoma were the major histological subtypes showed positive for PTHrP. In gastric cancer, only exceptional cases of adenocarcinoma associated with heterotopic ossification showed positive for PTHrP. In conclusion, PTHrP was expressed commonly in human cancer tissues regardless to the existence of hypercalcemia. Histological subtypes were well correlated to the expression of PTHrP and surrounding tissues could modify the production of PTHrP in cancer cells.
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Moseley JM, Kubota M, Diefenbach-Jagger H et al.: Parathyroid hormone-related protein purified from a human lung cancer cell line. Proc Natl Acad Sci USA 84: 5048–5052, 1987
Suva LJ, Winslow GA, Wettenhall REH et al.: A parathyroid hormone-related protein implicated in malignant hypercalcemia: cloning and expression. Science 237: 893–896, 1987
Kitazawa S, Fukase M, Kitazawa R et al.: Immunohistologic evaluation of parathyroid hormone-related protein in human lung cancer and normal tissue with newly developed monoclonal antibody. Cancer 67: 984–989, 1991
Asa SL, Henderson J, Goltzman D et al.: Parathyroid hormone-like peptide in normal and neoplastic human endocrine tissues. J Clin Endoclinol Metab 71: 1112–1118, 1990
Kitazawa R, Kitazawa S, Fukase M et al.: The expression of parathyroid hormone-related protein (PTHrP) in normal parathyroid: histochemistry and in situ hybridization Histochem 98: 211–215, 1992
Gotoh A, Kitazawa S, Mizuno Y et al.: Common expression of parathyroid hormone-related protein and no correlation of calcium level in renal cell carcinoma. Cancer 71: 2803–2806, 1993
Kitazawa S, Kitazawa R, Fukase M et al.: Immunohistochemical evaluation of parathyroid hormone-related protein (PTHrP) in the uterine cervix. Intl J Cancer 50: 731–735, 1992
Hoekman K, Lowik CWG, v.d. Ruit M et al.: Modulation of the production of a parathyroid-hormone-like protein in human squamous carcinoma cell line by interaction with fibroblast. Cancer Res 50: 3589–3598, 1990
Kohno N, Kitazawa S, Fukase M et al.: Expression of parathyroid hormone-related protein in human breast cancer with skeletal metastases. J Surg 1994: in press
Kitazawa S, Maeda S: Development of skeletal metastases. Clin Orthop Relat Res 1994: in press.
Vargus SJ, Gillespie MT, Powell GJ et al.: Localization of parathyroid hormone-related protein mRNA in breast cancer and metastatic lesions by in situ hybridization. J Bone Miner Res 8: 971–979, 1992
Rizzoli R, Bonjour JP: High extracellural calcium increases the production of parathyroid hormone-like activity by cultured Leidig tumor cells associated with hypercalcemia. J Bone Miner Res 4: 839–844, 1989
Yamamura-Idei Y, Kitazawa S, Kitazawa R et al.: Parathyroid hormone-related protein in gastric cancers with heterotopic ossification. Cancer 72: 1849–1852, 1993
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Kitazawa, R., Kitazawa, S., Fukunishi, H. et al. Clinicopathological relevance of parathyroid hormone-related protein in various types of cancer tissues. J Bone Miner Metab 12 (Suppl 1), S145–S151 (1994). https://doi.org/10.1007/BF02375693
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DOI: https://doi.org/10.1007/BF02375693