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Comparison of preparations of erythrocyte membranes and membrane proteins by SDS-Gel electrophoresis

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Résumé

Quelques préparations de membranes érythrocytaires et de leurs protéines extraites en phase aqueuse ont été comparées par électrophorèse sur gel de SDS-polyacrylamide. De faibles différences seulement ont été notées d'une préparation à l'autre. Les extraits obtenus à force ionique basse ou élevée contiennent tous la même protéine de poids moléculaire élevé (‘spectrine’) mais different quant à leur contenu en peptides de faible poids moléculaire. Les préparations obtenues à l'intérieur de chaque groupe s'avèrent fondamentalement semblables.

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Authors and Affiliations

  1. Department of Radiation Biology and Biophysics, University of Rochester School of Medicine and Dentistry, 14642, Rochester, New York, USA

    F. H. Kirkpatrick & P. L. LaCelle

Authors
  1. F. H. Kirkpatrick
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  2. P. L. LaCelle
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Additional information

We would like to thank Dr.Paul Bredderman for many discussions about gel electrophoresis. This work was supported by postdoctoral fellowship No. 34600 from the National Heart and Lung Institute, and by USPHS Grant No. AM1548-03. This paper is also based on work performed under contract with the U.S. Atomic Energy Commission at the University of Rochester Atomic Energy Project and has been assigned Report No. UR-3490-239.

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Kirkpatrick, F.H., LaCelle, P.L. Comparison of preparations of erythrocyte membranes and membrane proteins by SDS-Gel electrophoresis. Experientia 30, 140–142 (1974). https://doi.org/10.1007/BF01927694

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