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Myocardial lactate dehydrogenase isoenzyme distribution in the normal heart

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Summary

The isoenzyme pattern of the lactate-dehydrogenase (LDH) in different parts of the heart was measured by micro-isoelectric focusing. Samples were taken from the right and left auricle, the outer, middle and inner layer of the myocardial wall of both ventricles and from the papillary muscle of the left ventricle. The results show that the activity of LDHtotal and the isoenzyme pattern are different in the various parts of the heart. In both auricles the isoenzymes LDH3,4,5 were mostly found, whereas in the ventricles a significant increase of LDH1,2 could be seen. Additionally, there was a shift in the isoenzyme pattern from the outside to the inner part of the myocardium wall with a significant increase of LDH1.

Zusammenfassung

Mit Hilfe der mikroisoelektrischen Fokussierung wurde das Isoenzymmuster der Laktatdehydrogenase (LDH) in den verschiedenen Anteilen und Wandschichten des Herzens bestimmt. Untersucht wurden der rechte und linke. Vorhof, die äußere, mittlere und innere Wandschicht des linken Ventrikels. Hierbei wurden völlig verschiedene Enzymmuster und-aktivitäten gemessen. In beiden Vorhöfen überwogen die Isoenzyme LDH3,4,5, wohingegen in beiden Ventrikeln ein deutlicher Anstieg der LDH1,2 gegenüber den Vorhöfen beobachtet werden konnte. Innerhalb der Ventrikelwände wurden die höchsten LDH1-Konzentrationen in den endomyokardialen Schichten, die höchsten LDH5-Konzentrationen in den epikardialen Schichten gemessen.

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Author notes

  1. H. -P. Schultheiß, G. Bispink, V. Neuhoff & H. -D. Bolte

    Present address: Klinikum Großhadern, Medizinische Klinik I der Universität München, Marchioninistraße 15, D-8000, München 70, West Germany

Authors and Affiliations

  1. Forschungsstelle Neurochemie, Max-Planck-Institut für experimentelle Medizin, Göttingen

    H. -P. Schultheiß, G. Bispink, V. Neuhoff & H. -D. Bolte

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  1. H. -P. Schultheiß
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  2. G. Bispink
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  3. V. Neuhoff
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  4. H. -D. Bolte
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Schultheiß, H.P., Bispink, G., Neuhoff, V. et al. Myocardial lactate dehydrogenase isoenzyme distribution in the normal heart. Basic Res Cardiol 76, 681–689 (1981). https://doi.org/10.1007/BF01908058

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