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Effects of three avocado/soybean unsaponifiable mixtures on metalloproteinases, cytokines and prostaglandin E2 production by human articular chondrocytes

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Abstract

The in-vitro effects of avocado and soybean unsaponifiable residues on neutral metalloproteinase activity, cytokines and prostaglandin E2 (PGE2) production by human articular chondrocytes were investigated. Avocado and soybean unsaponifiable residues were mixed in three ratios: 1∶2 (A1S2), 2∶1 (A2S1) or 1∶1 (A2S2). Freshly isolated human chondrocytes were cultured for 72 h in the absence or presence of interleukin-1β (IL-1β) (17 ng/ml), with or without unsaponifiable residue mixtures at a concentration of 10 μg/ml. A/S unsaponifiable residues were also tested separately at concentrations of 3.3, 6.6 and 10 μg/ml. All A/S unsaponifiable mixtures reduced the spontaneous production of stromelysin, interleukin-6 (IL-6), interleukin-8 (IL-8) and prostaglandin E2 (PGE2) by chrondrocytes. At concentrations of 3.3 and 6.6 μg/ml, A/S residues, tested separately, were potent inhibitors of the production of IL-8 and PGE2. Nevertheless, only avocado residue inhibited IL-6 production at these concentrations. A/S unsaponifiable mixtures had a more pronounced inhibitory effect on cytokine production than avocado or soybean residues added alone. As anticipated, IL-1β induced a marked release of collagenase, stromelysin, IL-6, IL-8 and PGE2. A/S unsaponifiable mixtures partially reversed the IL-1 effects on chrondrocytes. These findings suggest a potential role for A/S unsaponifiable extracts in mitigating the deleterious effects of IL-1β on cartilage.

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References

  1. Rancurel A. Parfums, cosmétiques, Arômes 1985;61:91.

    Google Scholar 

  2. Farines M, Soulier J, Rancurel A, Montaudoin MG, Leborgne L. Influence of avocado oil processing on the nature of some unsaponifiable constituents. JAOCS 1995;72:473.

    Google Scholar 

  3. Baillet A. Pharmaceutical expert report. Courbevoie, France: Pharmascience, 1995 (unpublished data).

    Google Scholar 

  4. Robert AM. Miskulin M, Godeau G, Robert L. Pharmacologie du tissus conjonctif. Action des insaponifables d'avocat et de soja sur le métabolisme de la matrice intercellulaire. Gaz Med Fr 1975;82:2–6.

    Google Scholar 

  5. Dupurrat B, Lamberton J-N. Le traitement des sclérodermies par les insaponifiables d'huiles végétales. Arch Belg Dermat Symphil 1970;26:271–7.

    Google Scholar 

  6. Thiers H, Fayotte J, Zwingelstein G, Jouhanneteau J. L insaponifiable d'huiles végétales en solution alcoolique dans le traitement des sclérodermies et des états apparentés. IXéme Congr Ass Derm Syph Fr 1956;187–289 (Médecine & Hygiène, Genève).

    Google Scholar 

  7. Kerbel B, Clergeau-Guerithault S, Brion M. A scanning electron microscope study of experimental periodontal disease. Its induction and inhibition. J Periodontol 1975;46:27–35.

    PubMed  Google Scholar 

  8. Maziere B, Loyau G, Valat JP, Bourgeois P, Rosenberg S. 6-month symptomatic efficacy of avocado/soya unsaponifiable (ASU) in kene and hip osteoarthritis (KOA and HOA). Arthritis Rheum 1996;39(9 suppl):S227.

    Google Scholar 

  9. Lequesne M, Maheu E, Cadet C, Gaspard H, Dreiser RL. Effects of avocado/soya unsaponifiables (ASU) on joint space loss in hip osteoarthritis (HOA) over 2 years. A placebo controlled trial. Arthritis Rheum 1996;39(9 suppl):S227. fibronectin biosynthesis, elastase activity, cell proliferation and cell phenotype of rabbit chondrocytes by soja and avocado extracts. Xlle EULAR meeting (Budapest). Abstract blooklet 1991;FP 22:225.

    Google Scholar 

  10. Mauviel A, Daireaux M, Hartman DJ, Galera P, Loyau G, Pujol JP. Effets des insaponifiables d'avocat/soja (PIAS) sur la production de collagène par des cultures de synoviocytes, chondrocytes articulaires et fibroblastes dermiques. Rev Rhum 1989;56:207–13

    PubMed  Google Scholar 

  11. Harmand MF. Etude de l'action des insaponifiables d'avocat et de soja sur les cultures de chondrocytes articulaires. Gaz Med Fr 1985;92:1–3.

    Google Scholar 

  12. Mauviel A, Loyau G, Pujol JP. Effets des insaponifiables d'avocat/soja (Piasclédine) sur l'activité collagénolytique de cultures de synoviocytes rhumatoïdes humains et de chondrocytes articulaires de lapin traités par l'interleukine-1. Rev Rhum 1991;58:241–8.

    PubMed  Google Scholar 

  13. Lotz M, Blanco FJ, von Kempis J, Dudler J, Mier R, Villigher PM, et al. Cytokine regulation of chondrocyte functions. J Rheumatol 1995;22:104–12.

    Google Scholar 

  14. Martel-Pelletier J, Zafarullah M, Kodama S, Pelletier JP. In vitro effects of interleukin-1 on the synthesis of metalloproteinases, TIMP, plasminogen activators and inhibitors in human articular cartilage. J Rheumatol 1991;18:80–7.

    Google Scholar 

  15. Tiku ML, Liesch JB, Robertson FM. Production of hydrogen peroxide by rabbit articular chondrocytes. J Immunol 1990;145:690–6.

    PubMed  Google Scholar 

  16. Goldring MB, Birkhead J, Sandell LJ. Interleukin-1 suppresses expression of cartilage-specific type II and IX collagen and increases type I and III collagens in human chondrocytes. J Clin Invest 1988;82:2036–37.

    Google Scholar 

  17. Nietfield JJ, Wilbrink B, Den Otter W, Huber J, Huber-Bruning O. The effect of human interleukin 1 on proteoglycan metabolism in human and porcine cartilage explants. J Rheumatol 1990;17:818–26.

    PubMed  Google Scholar 

  18. Cawston E, Barrett AJ. A rapid and producible assay for collagenase using14C-acetylated collagen. Anal Biochem 1979;99:340–9.

    PubMed  Google Scholar 

  19. Gisslow M, McBride B. A rapid sensitive collagenase assay. Anal Biochem 1975;26:259–68.

    Google Scholar 

  20. Pardo A, Ramiriez R, Gutierrez-Kobeh L, Mendoza P, Bauer E, Selman M. Purification of procollagenase activator present in the medium of cultured guinea pig carrageenin granuloma. Connect Tiss Res 1991;26:259–68.

    Google Scholar 

  21. Serteyn D, Deby-Dupont G, Pincemail J, Mottard E, Philippart C, Lamy M. Equine post-anesthetic myositis: thromboxane, prostacyclin and prostaglandin E2 production. Vet Res Comm 1988;12:219–26.

    Google Scholar 

  22. De Groote D, Zangerle PF, Gevaert Y, Fassote MF, Beguin Y, Noizat-Pirenne F, et al.: Direct stimulation of cytokines (IL-1β, TNFα, IL-6, IL-2 IFNγ and GM-CSF) in whole blood. I. Comparison with isolated PBMC stimulation. Cytokine 1992; 4:239–48.

    PubMed  Google Scholar 

  23. Labarca C, Paigen K. A simple rapid and sensitive DNA assay procedure. Anal Biochem 1980;102:344–52.

    PubMed  Google Scholar 

  24. Maheu E, Le Loët X, Loyau G. 6-months symptomatic efficacy of avocado/soya unsaponifiables in osteoarthritis (OA) at the lower limb. Rev Rhum 1995;60:667–673.

    Google Scholar 

  25. Vignon E, Mathieu P, Richard M, Conrozier T. Etude de l'effet de Piascledine 300 sur le cartilage articulaire humain arthrosique in vivo. Symposium on the medical treatment of osteoarthritis (abstract booklet), Firenze, April 10–12, 1992.

  26. Harmand M-F. Modulation par les IAS de la matrice extracellulaire du cartilage humain. Etude in vitro. Rhumatologie Pratique 1993;100:1–6.

    Google Scholar 

  27. Okada Y, Konomi H, Yada T, Kimata K, Nagase H. Degradation of type IX collagen by matrix metalloproteinase 3 (stromelysin) from human rhumatoid synovial cells. FEBS Lett 1989;244:473–82.

    PubMed  Google Scholar 

  28. Wu JJ, Lark MW, Chun LE, Eyre DR. Sites of stromelysin cleavage in collagen type II, IX, X and XI of cartilage. J Biol Chem 1991;266:5625–34.

    PubMed  Google Scholar 

  29. Campbell IK, Roughley PJ, Mort JS. The action of human articular-cartilage metalloproteinase on proteoglycan and link protein. Similarities between products of degradation in situ and in vitro. Biochem J 1986;237:117–23.

    PubMed  Google Scholar 

  30. Murphy G, Hembry RM, Hughes C, Fosang AJ, Hardingham TE. Role and regulation of metalloproteinases in connective tissue turnover. Biochem Soc Trans 1990;18:812–21.

    PubMed  Google Scholar 

  31. Robert AM, Miskulin M, Godeau G. Biochemical pharmacology of connective tissues. II. Evolution of enzymatic activities in carrageenan granulomas. Influence of lipidic plant-extracts. ‘R’ Rhumatologie 1974;6:595–603.

    Google Scholar 

  32. Daniel A. Action des unsaponifiables d'avocat et de soja sur l'inflammation du tissu conjonctif. Gaz Med Fr 1979;86:2461–8.

    Google Scholar 

  33. Chin JE, Lin Y. Effects of recombinant human interleukin-1 β on rabbit articular chondrocytes. Stimulation of prostanoid release and inhibition of cell growth. Arthritis Rheum 1988;31:1290–6.

    PubMed  Google Scholar 

  34. Verschure PJ, Van Noorden CJF. The effects of interleukin-1 on articular cartilage destruction as observed in arthritic diseases, and its therapeutic control. Clin Exp Rheumatol 1990;8:303–39.

    PubMed  Google Scholar 

  35. Malenud CJ, Moskowitz RW, Papay RS. Correlation of the synthesis of protaglandin and cyclic AMP in monolayer cultures of rabbit articular chondrocytes. Biochem Biophys Acta 1982;715:70–7.

    PubMed  Google Scholar 

  36. Shimner M, Ghosh P, Taylor TK. N6, O2 dibutyryl adenosine 3′, 5′-monophosphate stimulated release of proteoglycans from cultured immature rabbit ear cartilage. Biochim biophys Acta 1976;437:94–104.

    PubMed  Google Scholar 

  37. Peveri P, Watlz A, Dewald B, Baggiolini M. A novel neutrophilactivating factor produced by human mononuclear phagocytes. J Exp Med 1988;167:1547–59.

    PubMed  Google Scholar 

  38. Gauldie J, Northemann W, Fey GH. IL-6 functions as an exocrine hormone in inflammation. Hepatocytes undergoing acute phase responses require exogenous IL-6. J Immunol 1990;144:3804–8.

    PubMed  Google Scholar 

  39. Lotz M, Guerne PA. Interleukin-6 induces the synthesis of tissue inhibitor of metalloproteases-1/erythroid potentiating activity (TIMP/EPA). J Biol Chem 1991;266:2017–20.

    PubMed  Google Scholar 

  40. Nietfeld JJ, Wilbrink B, Helle M, Van Roy LAM, Den Otter W, Swaak AJG, et al. Interleukin-1-induced interleukin-6 is required for the inhibition of proteoglycan synthesis by interleukin-1 in human articular cartilage. Arthritis Rheum 1990;33:1695–1701.

    PubMed  Google Scholar 

  41. Henrotin YE, De Groote DD, Labasse AH, Gaspar SL, Zheng SX Geenen VG, et al. Effects of exogenous IL-1β, TNFα, IL-6, IL-8, and LIF on cytokine production by articular chondrocytes. Osteoarthritis and Cartilage 1996;4:163–73.

    PubMed  Google Scholar 

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Authors and Affiliations

  1. Bone and Cartilage Metabolism Research Unit, Radioimmunoassay Laboratory, University Hospital, B35, B-4000, Liège, Belgium

    Y. E. Henrotin, A. H. Labasse, S. X. Zheng & J. Y. L. Reginster

  2. Biosource Europe, B35, B-4000, Fleurus, Belgium

    J. M. Jaspar & D. D. De Groote

  3. Phannascience Laboratories, Boulevard de la Mission Marchand, B35, B-4000, Courbevoie, France

    G. B. Guillou

Authors
  1. Y. E. Henrotin
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  2. A. H. Labasse
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  3. J. M. Jaspar
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  4. D. D. De Groote
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  6. G. B. Guillou
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  7. J. Y. L. Reginster
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Henrotin, Y.E., Labasse, A.H., Jaspar, J.M. et al. Effects of three avocado/soybean unsaponifiable mixtures on metalloproteinases, cytokines and prostaglandin E2 production by human articular chondrocytes. Clin Rheumatol 17, 31–39 (1998). https://doi.org/10.1007/BF01450955

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  • DOI: https://doi.org/10.1007/BF01450955

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