Skip to main content
SpringerLink
Log in

Selective retention of organic phosphate esters and phosphonates on aluminium oxide

  • Short Papers
  • Published:
Bioscience Reports

Abstract

Compounds containing the −PO3H2 function, such as monoesters of phosphoric acid and phosphonic acids, specifically bind to aluminium oxide in aqueous solution under experimental conditions where non-phosphorylated compounds are completely desorbed. The bound organic phosphate can be specifically displaced by aqueous solution of inorganic phosphates thus allowing their separation or detection by a technique similar to that of affinity chromatography. The consequences of this finding for phosphate compound biochemistry are discussed.

This is a preview of subscription content, log in via an institution to check access.

Access this article

Log in via an institution

Price excludes VAT (USA)
Tax calculation will be finalised during checkout.

Instant access to the full article PDF.

Institutional subscriptions

References

  1. Cohen, P. (1982).Nature 296:613–620.

    Google Scholar 

  2. “Protein Phosphorylation”, Cold Spring Harbor Symp. (Rosen, O. M., and Krebs, E. G., Eds.), 1981, vols. 1 and 2.

  3. Roach, P. J. (1984).Methods in Enzymology 107:81–101.

    Google Scholar 

  4. Ingebritsen, T. S., and Cohen, P. (1983).Eur. J. Biochem. 132:255–262.

    Google Scholar 

  5. Colowick, S. P. (1955).Methods in Enzymology 1:90–98.

    Google Scholar 

  6. White, A. A., and Zenser, T. V. (1971).Anal. Biochem. 41:372–396.

    Google Scholar 

  7. Ramachandran, J. (1971).Anal. Biochem. 43:227–239.

    Google Scholar 

  8. Reimerdes, E. H., and Klostermeyer, H. (1976).Methods in Enzymology 45:26–28.

    Google Scholar 

  9. Previero, A., Cavadore, J. C., Torreilles, J., and Coletti-Previero, M-A. (1979).Biochim. Biophys. Acta 581:276–282.

    Google Scholar 

  10. Kemp, B. E. (1979).J. Biol. Chem. 254:2638–2641.

    Google Scholar 

  11. Peri, J. B. (1965).J. Phys. Chem. 69:211–220.

    Google Scholar 

  12. Stahl, E. (1969).Thin-Layer Chromatography, Springer-Verlag (Berlin), p. 26.

    Google Scholar 

  13. Chibeta, I. (1978).Immobilized Enzymes, John Wiley & Sons (New York), p. 13.

    Google Scholar 

  14. Templeton, M. K., and Weinberg, W. H. (1985).J. Am. Chem. Soc. 107:774–779.

    Google Scholar 

  15. Miyamoto, K., Fujii, T., and Miura, T. (1971).J. Ferment. Technol. 49:565–573.

    Google Scholar 

  16. Solomon, B., and Levin, Y. (1975)Biotechnol. Bioeng. 17:1323–1328.

    Google Scholar 

  17. Nicolas, J. C., Boussioux, A. M., Balguer, P., Badia, E., Coletti-Previero, M.-A., and Previero, A. (In preparation.)

Download references

Author information

Authors and Affiliations

  1. INSERM U 58, 60 rue des Navacelles, 34100, Montpellier, France

    M-A. Coletti-Previero, M. Pugnière, H. Mattras, J. C. Nicolas & A. Previero

Authors
  1. M-A. Coletti-Previero
    View author publications

    You can also search for this author in PubMed Google Scholar

  2. M. Pugnière
    View author publications

    You can also search for this author in PubMed Google Scholar

  3. H. Mattras
    View author publications

    You can also search for this author in PubMed Google Scholar

  4. J. C. Nicolas
    View author publications

    You can also search for this author in PubMed Google Scholar

  5. A. Previero
    View author publications

    You can also search for this author in PubMed Google Scholar

Rights and permissions

Reprints and permissions

About this article

Cite this article

Coletti-Previero, MA., Pugnière, M., Mattras, H. et al. Selective retention of organic phosphate esters and phosphonates on aluminium oxide. Biosci Rep 6, 477–483 (1986). https://doi.org/10.1007/BF01116139

Download citation

  • Received:

  • Issue Date:

  • DOI: https://doi.org/10.1007/BF01116139

Key Words

Search

Navigation