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Reduced infarct size in the rabbit heartin vivo by ethylisopropyl-amiloride. A role for Na+/H+ exchange

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Abstract

Inhibition of Na+/H+ exchange with amiloride analogues has been shown to offer functional protection during ischemia and reperfusion and reduce infarct size in isolated rat hearts and intact pigs. The aim of the present study was to examine if pre- or postischemic treatment with ethylisopropylamiloride (EIPA), a selective Na+/H+ exchange inhibitor, could reduce infarct size in anin situ rabbit model of regional ischemia and reperfusion. Anesthetized, open-chest rabbits were subjected to 30 min of regional ischemia and 180 min of reperfusion. The risk zone was determined by fluorescent particles, and infarct size was determined by TTC staining. Preischemic treatment with EIPA (0.65 mg/kg) significantly reduced infarct size from 45.8±3.5% of the risk zone in the control group to 10.6±3.1% (p<0.01). EIPA-treatment during the first part of the reperfusion period did not reduced infarct size compared to controls (41.9±3.5%). We conclude that EIPA, when administered prior to ischemia, reduces infarct size in the rabbit heartin situ, a protection most likely due to inhibition of Na+/H+ exchange.

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Authors and Affiliations

  1. Department of Medical Physiology Institute of Medical Biology, University of Tromso, 9037, Tromso, Norway

    E. Bugge, J. Munch-Ellingsen & K. Ytrehus

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  1. E. Bugge
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  2. J. Munch-Ellingsen
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  3. K. Ytrehus
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Bugge, E., Munch-Ellingsen, J. & Ytrehus, K. Reduced infarct size in the rabbit heartin vivo by ethylisopropyl-amiloride. A role for Na+/H+ exchange. Basic Res Cardiol 91, 203–209 (1996). https://doi.org/10.1007/BF00788906

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  • DOI: https://doi.org/10.1007/BF00788906

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