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A rapid and efficient method to remove labelled molecular probes from nucleic acids immobilized on solid supports

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Abstract

A rapid and efficient method is described for the removal of radio-active molecular probes from nucleic acids immobilized on nylon membranes. This method involves boiling in distilled water in a microwave oven. This procedure can be completed within ten minutes, does not require the use of any buffers or reagents, and produces results comparable with conventional buffer-wash procedures recommended by the suppliers of the transfer membranes.

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Authors and Affiliations

  1. Department of Biology, Queen's University, K7L 3N6, Kingston, Ontario, Canada

    Charlene P. Wight, David T. Dennis & Reginald H. Lau

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  1. Charlene P. Wight
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  2. David T. Dennis
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  3. Reginald H. Lau
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Wight, C.P., Dennis, D.T. & Lau, R.H. A rapid and efficient method to remove labelled molecular probes from nucleic acids immobilized on solid supports. Mol Biol Rep 11, 173–175 (1986). https://doi.org/10.1007/BF00419738

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  • DOI: https://doi.org/10.1007/BF00419738

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