Summary
An enzyme immunoassay technique combined with Western blotting is described to demonstrate thyroxine-binding globulin (TBG) by isoelectric focusing in thin-layer polyacrylamide gels with 8mol/l urea. Quantitative evaluation was by laser densitometry. No genetic charge variants of TBG were encountered in a sample of 840 unrelated individuals from southwestern Germany. There was no correlation between structural and quantitative variations in the TBG protein. Results from a family with quantitative TBG deficiency strongly support the postulated X-linked mode of inheritance. The method described can be considered as an additional diagnostic tool in thyroid evaluation.
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References
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Luckenbach, C., Kömpf, J. & Ritter, H. Genetic studies on human thyroxine-binding globulin (TBG). Hum Genet 84, 368–370 (1990). https://doi.org/10.1007/BF00196237
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DOI: https://doi.org/10.1007/BF00196237