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Microwave histoprocessing of bone marrow trephine biopsies

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Summary

In recent years, the microwave oven has been increasingly used in the pathology laboratory for processing of tissue for diagnostic purposes with a remarkable reduction in processing time and also reports of excellent morphology and immunohistochemistry. We evaluated some of these processes on post mortem bone marrow trephine biopsies and describe a novel way of processing these biopsies in the microwave oven.

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References

  • Boon, M. E. & Kok, L. P. (1988) Microwave Cookbook of Pathology, 2nd edn. Leiden: Coulomb Press.

    Google Scholar 

  • Boon, M. E., Kok, L. P. & Ouwerkerk-Noordam, E. (1986) Microwave-stimulated diffusion for fast processing of tissue: reduced dehydrating, clearing, and impregnating times. Histopathology 10, 303–9.

    Google Scholar 

  • Boon, M. E., Gerrits, P. O., Morrlag, H. E., Nieuwenhuis, P. & Kok, L. P. (1988) Formaldehyde fixation and microwave irradiation. Histochem. J. 20, 313–22.

    Google Scholar 

  • Curran, R. C. & Gregory, J. (1980) Effects of fixation and processing on immunohistochemical demonstration of immunoglobulin in paraffin sections of tonsil and bone marrow. J. Clin. Pathol. 33, 1047–57.

    Google Scholar 

  • Findlay, A. B. (1977) Bone marrow changes in the post mortem interval. J. Forensic Sci. Soc. 16, 213–8.

    Google Scholar 

  • Gordon, H. W. & Daniel, E. J. (1974) Preliminary report: microwave fixation of human tissues. Am. J. Med. Technol. 40, 441–2.

    Google Scholar 

  • Hellström, S. & Nilsson, M. (1992) The microwave oven in temporal bone research. Acta Otolaryngol. (Stockh) (suppl) 493, 15–18.

    Google Scholar 

  • Hellström, S., Tengblad, A., Johansson, C., Hedlung, U. & Axelsson, E. (1990) An improved technique for hyaloronan histochemistry using microwave irradiation. Histochem. J. 22, 677–82.

    Google Scholar 

  • Holgate, C. S., Jackson, P., Pollard, K., Lunny, D. & Bird, C. C. (1986) Effect of fixation on T and B lymphocyte surface membrane antigen demonstration in paraffin processed tissue. J. Pathol. 149, 293–300.

    Google Scholar 

  • Hopwood, D., Yeaman, G. & Milne, G. (1988) Differentiating the effects of microwave and heat on tissue proteins and their crosslinking by formaldehyde. Histochem. J. 20, 341–6.

    Google Scholar 

  • Kok, L. P. & Boon, M. E. (1990) Microwaves for microscopy. J. Microsc. 158, 291–322.

    Google Scholar 

  • Kok, L. P., Boon, M. E. & Suurmeijer, A. J. H. (1987) Major improvement in microscope-image quality of cryostat sections. Am. J. Clin. Pathol. 88, 620–3.

    Google Scholar 

  • Kok, L. P., Visser, P. E. & Boon, M. E. (1988) Histoprocessing with the microwave oven: an update. Histochem. J. 20, 323–8.

    Google Scholar 

  • Lai, F. M-M., Lai, K. N., Chew, E. C. & Lee, J. C. K. (1987) Microwave fixation in diagnostic renal pathology. Pathology 19, 17–21.

    Google Scholar 

  • Leong, A. S-Y. (1988) Microwave irradiation in histopathology. Pathol. Annu. 23, 213–34.

    Google Scholar 

  • Leong, A. S-Y. (1991) Microwave fixation and rapid processing in a large throughput histopathology laboratory. Pathology 23, 271–3.

    Google Scholar 

  • Leong, A. S-Y. & Milios, J. (1986) Rapid immunoperoxidase staining of lymphocyte antigens using microwave irradiation. J. Pathol. 148, 183–7.

    Google Scholar 

  • Leong, A. S-Y. & Milios, J. (1990) Accelerated immunohistochemical staining by microwaves. J. Pathol. 161, 327–34.

    Google Scholar 

  • Leong, A. S-Y. & Pulbrook, S. (1989) Microwave-stimulated staining of reticulin fibers in plastic sections by an ammoniacal silver nitrate method. J. Histotechnol. 12, 289–91.

    Google Scholar 

  • Leong, A. S-Y., Daymon, M. E. & Milios, J. (1985) Microwave irradiation as a form of fixation for light and electron microscopy. J. Pathol. 146, 313–21.

    Google Scholar 

  • Login, G. R. (1978) Microwave fixation versus formalin fixation of surgical and autopsy tissue. Am. J. Med. Technol. 44, 435–7.

    Google Scholar 

  • Login, G. R., Schnitt, S. J. & Dvorak, A. M. (1987) Methods in laboratory investigations. Rapid microwave fixation of human tissue for light microscopic immunoperoxidase identification of diagnostically useful antigens. Lab. Invest. 57, 585–91.

    Google Scholar 

  • Mayers, C. P. (1970) Histological fixation by microwave heating. J. Clin. Pathol. 23, 273–5.

    Google Scholar 

  • Medawar, P. B. (1941) III. The rate of penetration of fixatives. J. R. Microsc. Soc. 61, 46–57.

    Google Scholar 

  • Moran, R. A., Nelson, F., Jagirdar, J. & Paronetto, F. (1988) Application of microwave irradiation to immunohistochemistry: preservation of antigens of the extracellular matrix. Stain Technol. 63, 263–9.

    Google Scholar 

  • Pelstring, R. J., Allred, C., Esther, R. J., Lampkin, S. R. & Banks, P. M. (1991) Differential antigen preservation during tissue autolysis. Hum. Pathol. 22, 237–41.

    Google Scholar 

  • Tornero, G., Latta, L. L. & Godoy, G. (1991) Use of microwave radiation for the histological study of bone canaliculi. J. Histotechnol. 14, 27–30.

    Google Scholar 

  • Wittekind, D. (1979) On the nature of Romanowsky dyes and the Romanowsky-Giemsa effect. Clin. Lab. Haematol. 1, 247–62.

    Google Scholar 

  • Wong, F. T. S. (1990) Microwave stimulated diffusion method for processing oral mucosal tissue of hamster and human being. J. Histotechnol. 13, 127–9.

    Google Scholar 

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Louw, I., De Beer, D.P. & Du Plessis, M.J. Microwave histoprocessing of bone marrow trephine biopsies. Histochem J 26, 487–494 (1994). https://doi.org/10.1007/BF00157894

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  • DOI: https://doi.org/10.1007/BF00157894

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