Abstract
The biotransformation of toluene, benzene and naphthalene was examined in anaerobic sediment columns. Five columns filled with a mixture of sediments were operated in the presence of bicarbonate, sulfate, iron, manganese, or nitrate as electron acceptor. The columns were continuously percolated with a mixture of the three organic compounds (individual concentrations 25–200 μM) at 20°C.
Toluene was transformed readily (within 1 to 2 months) under all redox conditions tested. Benzene was recalcitrant over the test period of 375–525 days in all five columns. Naphthalene was partly transformed in the column with nitrate or manganese as electron acceptor present; the addition of benzoate had a positive effect in the column with nitrate. In the column with sulfate, the majority of the added naphthalene disappeared. No effect was observed after adding and omitting an easier degradable substrate. [14C]naphthalene was used to confirm this disappearance to be the result of degradation; two third of the naphthalene was converted to CO2.
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References
Aihara J (1992) Why aromatic compounds are stable. Scientific American 266: 62–68
Al-bashir B, Cseh T, Leduc R & Samson R (1990) Effect of soil contaminant interactions on the biodegradation of naphthalene in flooded soil under denitrifying conditions. Appl. Microbiol. Biotechnol. 34: 414–419
Altenschmidt U & Fuchs G (1991) Anaerobic degradation of toluene in denitrifying Pseudomonas sp.: indication for toluene methyl-hydroxylation and benzyl-CoA as central aromatic intermediate. Arch. Microbiol. 156: 152–158
Altenschmidt U & Fuchs G (1992) Anaerobic toluene oxidation to benzyl alcohol and benzaldehyde in a denitrifying Pseudomonas strain. J. Bacteriol. 174: 4860–4862
Beller HR, Reinhard M & Grbić-Galić D (1992) Metabolic by-products of anaerobic toluene degradation by sulfate-reducing enrichment cultures. Appl. Environ. Microbiol. 58: 3192–3195
Bosma TNP, van derMeer JR, Schraa G, Tros M & Zehnder AJB (1988) Reductive dechlorination of all trichloro- and dichlorobenzene isomers. FEMS Microbiol. Ecol. 53: 223–229
Burdige DJ & Nealson KH (1985) Microbial manganese-reduction by enrichment cultures from coastal marine sediments. Appl. Environ. Microbiol. 50: 491–497
deBruin WP, Kotterman MJJ, Posthumus MA, Schraa G & Zehnder AJB (1992) Complete biological reductive transformation of tetrachloroethene to ethane. Appl. Environ. Microbiol. 58: 1996–2000
Dolfing J, Zeyer J, Binder-Eicher P & Schwarzenbauch RP (1990) Isolation and characterization of a bacterium that mineralizes toluene in the absence of molecular oxygen. Arch. Microbiol. 154: 336–341
Edwards EA & Grbić-Galić D (1992a) Complete mineralization of benzene by aquifer microorganisms under strictly anaerobic conditions. Appl. Environ. Microbiol. 58: 2663–2666
Edwards EA, Wills LE, Reinhard M & Grbić-Galić D (1992b) Anaerobic degradation of toluene and xylene by aquifer microorganisms under sulfate-reducing conditions. Appl. Environ. Microbiol. 58: 794–800
Evans PJ, Ling W, Goldschmidt B, Ritter ER & Young LY (1992) Metabolites formed during anaerobic transformation of toluene and ortho-xylene and their proposed relationship to the initial steps of toluene mineralization. Appl. Environ. Microbiol. 58: 496–501
Evans PJ, Mang DT, Kim KS & Young LY (1991) Anaerobic degradation of toluene by a denitrifying bacterium. Appl. Environ. Microbiol. 57: 1139–1145
Fuchs G, Mohamed MES, Altenschmidt U, Koch J, Lack A, Brackmann R, Lochmeyer C & Oswald B (1994) Biochemistry of anaerobic degradation of aromatic compounds. In: C.Ratledge (Ed) Biochemistry of Microbial Degradation (pp 513–553). Kluwer Academic Publishers, Dordrecht.
Gibson DT & Subramanian V (1984) Microbial degradation of aromatic hydocarbons. In: D. T.Gibson (Ed) Microbial Degradation of Organic Compounds (pp 181–252). Marcel Dekker, Inc., New York and Basel.
Grbić-Galić D (1990) Anaerobic microbial transformation of nonoxygenated aromatic and alicyclic compounds in soil, subsurface and freshwater sediments. In: J. M. Bollag and G. Stotzky (Eds) Soil Biochemistry and Microbiology, Vol 6 (pp 117–189). Marcel Dekker, Inc.,
Grbić-Galić D & Vogel TM (1987) Transformation of toluene and benzene by mixed methanogenic cultures. Appl. Environ. Microbiol. 53: 254–260
Holliger C, Schraa G, Stams AJM & Zehnder AJB (1993) A highly purified bacterium couples the reductive dechlorination of tetrachloroethene to growth. Appl. Environ. Microbiol. 59: 2991–2997
Knowles R (1982) Denitrification. Microbiology Reviews 46: 43–70
Langenhoff AAM, Zehnder AJB, & Schraa G (1996) Microbial reduction of manganese coupled to toluene oxidation. FEMS Microbiol. Ecol. submitted
Lovley DR, Coates JD, Woodward JC & Phillips EJP (1995) Benzene oxidation coupled to sulfate reduction. Appl. Environ. Microbiol. 61: 953–958
Lovley DR & Lonergan DJ (1990) Anaerobic oxidation of toluene, phenol and p-cresol by the dissimilatory iron-reducing organism, GS-15. Appl. Environ. Microbiol. 56: 1858–1864
Lovley DR & Phillips EJP (1986) Organic matter mineralization with reduction of ferric iron in anaerobic sediments. Appl. Environ. Microbiol. 51: 683–689
Lovley DR, Woodward JC & Chapelle FH (1994) Stimulated anoxic biodegradation of aromatic hydrocarbons using fe(III) ligands. Nature 370: 128–131
Middeldorp PJM, Jaspers M, Zehnder AJB & Schraa G (1996) Biotransformation of α-, β-, γ- and δ-hexachlorocyclohexane to benzene and chlorobenzene under methanogenic conditions. Environmental Science and Technology accepted
Mihelcic JR & Luthy RG (1988a) Degradation of aromatic hydrocarbon compounds under various redox conditions in soil-water systems. Appl. Environ. Microbiol. 54: 1182–1187
Mihelcic JR & Luthy RG (1988b) Microbial degradation of acenaphthalene and naphthalene under denitrification conditions in soil-water systems. Appl. Environ. Microbiol. 54: 1188–1198
Mihelcic JR & Luthy RG (1991) Sorption and microbial degradation of naphthalene in soil-water suspensions under denitrification conditions. Environmental Science and Technology 25: 169–177
Rabus R, Nordhaus R, Ludwig W & Widdel F (1993) Complete oxidation of toluene under strictly anoxic conditions by a new sulfate-reducing bacterium. Appl. Environ. Microbiol. 59: 1444–1451
RIWA (1993) Ur ernstig verontreinigd, IAZI van DSM onterecht beschuldigd. H2O 26: 659–660
Schocher RJ, Seyfried B, Vazquez F & Zeyer J (1991) Anaerobic degradation of toluene by pure cultures of denitrifying bacteria. Arch. Microbiol. 157: 7–12
Smith MR (1990) The biodegradation of aromatic bydrocarbons by bacteria. Biodegradation 1: 191–206
Smith MR (1994) The physiology of aromatic hydrocarbon degrading bacteria. In C.Ratledge (Ed) Biochemistry of Microbial Degradation (pp 347–378). Kluwer Academic Publishers, Dordrecht.
Stumm W & Morgan JJ (1981) Aquatic chemistry. John Wiley and Sons, Inc., New York.
Vogel TM & Grbić-Galić D (1986) Incorporation of oxygen from water into toluene and benzene during anaerobic fermentative transformation. Appl. Environ. Microbiol. 52: 200–202
Weast RC (1971–1972) Handbook of chemistry and physics. The Chemical Rubber Co., Cleveland.
Zedeck MS (1980) Polycyclic aromatic hydrocarbons — a review. Journal of Environmental Pathology and Toxicology 3: 537–567
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Langenhoff, A.A.M., Zehnder, A.J.B. & Schraa, G. Behaviour of toluene, benzene and naphthalene under anaerobic conditions in sediment columns. Biodegradation 7, 267–274 (1996). https://doi.org/10.1007/BF00058186
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DOI: https://doi.org/10.1007/BF00058186