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Venom Toxins of Fire Ants

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Venom Genomics and Proteomics

Abstract

Fire ants are aggressive invasive insects spread around the world via ship cargo, mainly originating from the United States. They can cause severe impacts on human activities and the environment. This chapter presents an overview of what is known about fire ant venom, ending with different open possibilities for investigation in this topic. For decades, studies on fire ant venoms have been limited in scope because of the technical difficulties in obtaining enough samples for analysis and bioassays; yet now there is one simple, effective, published method for extracting venom from whole colonies. Fire ant venom is mainly composed of a mixture of >95 % bioactive piperidine alkaloids and 0.01 % of proteins, which comprise mainly allergens, phospholipases, and neurotoxins. The alkaloids of fire ants, generally named solenopsins, are well known for their antifungal and insecticidal properties; however, many have also been suggested as promising alternatives for other biomedical applications, such as the treatment of parasitemia and various neurological diseases. The venom proteins of fire ants remain only superficially studied, as most published literature focuses on just four allergens. Crucially, others may contain compounds of interest to immunotherapy or even play a central role in aspects of social organization.

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References

  • Allen CR, Forys EA, Rice KG, Wojcik DP. Effects of fire ants (Hymenoptera: Formicidae) on hatching turtles and prevalence of fire ants on sea turtle nesting beaches in Florida. Fla Entomol. 2001;84:250–3.

    Article  Google Scholar 

  • Anderson JM, Oliveira F, Kamhawi S, Mans BJ, Reynoso D, Seitz AE, Lawyer P, Garfield M, Pham M, Valenzuela JG. Comparative salivary gland transcriptomics of sandfly vectors of visceral leishmaniasis. BMC Genomics. 2006;7:52.

    Article  PubMed  PubMed Central  Google Scholar 

  • Arbiser JL, Kau T, Konar M, Narra K, Ramchandran R, Summers SA, Vlahos CJ, Ye K, Perry BN, Matter W, Fischl A, Cook J, Silver PA, Bain J, Cohen P, Whitmire D, Furness S, Govindarajan B, Bowen JP. Solenopsin the alkaloidal component of the fire ant (Solenopsis invicta) is a naturally occurring inhibitor of phosphatidylinositol-3-kinase signaling and angiogenesis. Blood. 2007;109(2):560–5.

    Article  PubMed  CAS  PubMed Central  Google Scholar 

  • Baer H, Liu TY, Anderson MC, Blum M, Schmidt WH, James FJ. Protein components of fire any venom. Toxicon. 1979;17:397–405.

    Article  PubMed  CAS  Google Scholar 

  • Bates HW. The naturalist on the river Amazons. London: Murray; 1856.

    Google Scholar 

  • Blum MSJR, Walker PS, Callahan XX, Novak AF. Chemical insecticidal and antibiotic properties of fire ant venom. Science. 1958;128:306–7.

    Article  PubMed  CAS  Google Scholar 

  • Borer AS, Wassmann P, Schmidt M, Hoffman DR, Zhou JJ, Wright C, Schirmer T, Marković-Housley Z. Crystal structure of Sol i 2: a major allergen from fire ant venom. J Mol Biol. 2012;415:635–48.

    Article  PubMed  CAS  Google Scholar 

  • Chalcraft DR, Andrews RM. Predation on lizard eggs by ants: species interactions in a variable physical environment. Oecologia. 1999;119:285–92.

    Article  Google Scholar 

  • Chen L, Fadamiro HY. Re-investigation of venom chemistry of Solenopsis fire ants. I. Identification of novel alkaloids in S. richteri. Toxicon. 2009;53:469–78.

    Article  PubMed  CAS  Google Scholar 

  • Chen J, Cantrell CL, Shang HW, Rojas MG. Piperideine alkaloids from the poison gland of the red imported fire ant (Hymenoptera: Formicidae). J Agric Food Chem. 2009;57:3128–33.

    Article  PubMed  CAS  Google Scholar 

  • Drees BM. Red imported fire ant predation on nestlings of colonial waterbirds. Southwest Entomol. 1994;19:355–9.

    Google Scholar 

  • Fox EGP, Bueno OC, Yabuki AT, Jesus CM, Solis DR, Rossi ML, Nogueira NL. General morphology and ultrastructure of the venom apparatus and convoluted gland of the fire ant, Solenopsis saevissima. J Insect Sci. 2010;10(24):1–11.

    Article  CAS  Google Scholar 

  • Fox EGP, Pianaro A, Solis DR, Delabie JHC, Vairo BC, Machado EA, Correa OC. Intraspecific and Intracolonial Variation in the Profile of Venom Alkaloids and Cuticular Hydrocarbons of the Fire Ant Solenopsis saevissima Smith (Hymenoptera: Formicidae). Psyche. 2012;2012:1–10.

    Google Scholar 

  • Fox EGP, Solis DR, Santos LD, Pinto JRAS, Menegasso ARS, Silva RCMC, Palma MS, Correa OCB, Machado EA. A simple, rapid method for the extraction of whole fire ant venom (Insecta: Formicidae: Solenopsis). Toxicon. 2013;65:5–8.

    Article  CAS  Google Scholar 

  • Haight KL, Tschinkel WR. Patterns of venom synthesis ans use in the fire ant, Solenopsis invicta. Toxicon. 2003;42:673–682.

    Article  CAS  Google Scholar 

  • Haight KL. Defensiveness of the fire ant Solenopsis invicta is increased during colony rafting. Insect Soc. 2006;53(1):32–6.

    Article  Google Scholar 

  • Hoffman DR. Allergens in Hymenoptera venom XXIV: the amino acid sequences of imported fire ant venom allergens Sol i II Sol i III and Sol i IV. J Allergy Clin Immunol. 1993;91:71–8.

    Article  PubMed  CAS  Google Scholar 

  • Hoffman DR. Fire ant and venom allergy. Allergy. 1995;50:535–44.

    Article  PubMed  CAS  Google Scholar 

  • Hoffman DR, Dove DE, Jacobson RS. Allergens in Hymenoptera venom: XX. Isolation of four allergens from imported fire ant (Solenopsis invicta) venom. J Allergy Clin Immunol. 1988;82:818–27.

    Article  PubMed  CAS  Google Scholar 

  • Hoffman DR, Smith AM, Schmidt M, Moffitt JE, Guralnick M. Allergens in Hymenoptera venom. XXII. Comparison of venoms from two species of imported fire ants Solenopsis invicta and richteri. J Allergy Clin Immunol. 1990;85:988–96.

    Article  PubMed  CAS  Google Scholar 

  • Hoffman DR, Sakell RH, Schmidt M. Sol i 1 the phospholipase allergen of imported fire ant venom. J Allergy Clin Immunol. 2005;115:611–16.

    Article  PubMed  CAS  Google Scholar 

  • Howell G, Butler J, DeShazo RD, Farley JM, Liu HL, Nanayakkara NPD, Yates A, Yi GB, Rockhold RW. Cardiodepressant and neurologic actions of Solenopsis invicta (imported fire ant) venom alkaloids. Ann Allergy Asthma Immunol. 2005;94:380–6.

    Article  PubMed  CAS  Google Scholar 

  • Javors MA, Zhou W, Maas Jr JW, Han S, Keenan RW. Effects of fire ant venom alkaloids on platelet and neutrophil function. Life Sci. 1993;53:1105–12.

    Article  PubMed  CAS  Google Scholar 

  • Jouvenaz EP, Blum MS, MacConnell JG. Antibacterial activity of venom alkaloids from the imported fire ant Solenopsis invicta Buren. Antimicrob Agents Chemother. 1972;2:291–3.

    Article  PubMed  CAS  PubMed Central  Google Scholar 

  • Kemp SF, deShazo RD, Moffitt JE, Williams DF, Buhner WA. Expanding habitat of the imported fire ant (Solenopsis invicta): a public health concern. J Allergy Clin Immunol. 2000;105:683–91.

    Article  PubMed  CAS  Google Scholar 

  • Koch RB, Desaiah D, Foster D, Ahmed K. Effect of piperidines and fire ant venom on ATPase activities from brain homogenate fractions and the characterization of Na+ -K+ ATPase inhibition. Biochem Pharmacol. 1977;26:983–5.

    Article  PubMed  CAS  Google Scholar 

  • Lai LC, Chang YY, Huang RN, Wu WJ. Comparative toxicity of two fire ant venoms to Spodoptera litura larvae. Sociobiology. 2010;56:653–63.

    Google Scholar 

  • Leclercq S, Daloze D, Braekman J-C. A synthesis of the fire ant alkaloids solenopsins. Org Prep Proced Int. 1996a;28(5):499.

    Article  Google Scholar 

  • Leclercq S, Braekman JC, Daloze D, Pasteels J, Van Der Meer RK. Biosynthesis of the solenopsins venom alkaloids of the fire ants. Naturwissenschaften. 1996b;83(5):222–5.

    Article  CAS  Google Scholar 

  • Li S, Jin X, Chen J. Effects of piperidine and piperideine alkaloids from the venom of red imported fire ants Solenopsis invicta Buren on Pythium ultimum Trow growth in vitro and the application of piperideine alkaloids to control cucumber damping-off in the greenhouse. Pest Manag Sci. 2012;68:1546–52.

    Article  PubMed  CAS  Google Scholar 

  • Lind NK. Mechanism of action of fire ant (Solenopsis) venoms. I. Lytic release of histamine from mast cells. Toxicon. 1982;20:831–40.

    Article  PubMed  CAS  Google Scholar 

  • McDonald M. Reds under your feet (interview with Robert Vander Meer). New Sci. 2006;189(2538):50.

    Google Scholar 

  • Mendes S. Formigas carnívoras atacam cidade amazônica [Internet]; 2008 (cited July 2014). Available from http://memoria.ebc.com.br/agenciabrasil/noticia/2008-01-14/formigas-carnivoras-atacam-cidade-do-amazonas

  • More DR, Kohlmeier RE, Hoffman DR. Fatal anaphylaxis to indoor native fire ant stings in an infant. Am J Forensic Med Pathol. 2008;29(1):62–3.

    Article  PubMed  Google Scholar 

  • Morrison LW, Porter SD, Daniels E, Korzuhkin MD. Potential global range expansion of the invasive fire ant, Solenopsis invicta. Biol Invasions. 2004;6:183–91.

    Article  Google Scholar 

  • Ownby DR. Prevalence of allergic sensitization to imported fire ants in children living in an endemic region of the southeastern United States. Ann Allergy Asthma Immunol. 2008;100:54–8.

    Article  PubMed  Google Scholar 

  • Padavattan S, Schmidt M, Hoffman DR, Marković-Housley Z. Crystal Structure of the major allergen from fire ant venom Sol i 3. J Mol Biol. 2008;383:178–85.

    Article  PubMed  CAS  Google Scholar 

  • Park J, Kaufmann GF, Bowen JP, Arbiser JL, Janda KD. Solenopsin A, a venom alkaloids from the fire ant Solenopsis invicta, inhibits quorum-sensing signaling in Pseudomonas aeruginosa. Journal of Infectious Diseases. 2008;198(8):1198–1201.

    Article  PubMed  CAS  Google Scholar 

  • Pelletier G, Constantineau-Forget L, Charette AB. Directed functionalization of 12-dihydropyridines: stereoselective synthesis of 26-disubstituted piperidines. Chem Commun. 2014;50:6883–5.

    Article  CAS  Google Scholar 

  • Pianaro A, Fox EGP, Bueno OC, Marsaioli AJ. Rapid configuration analysis of the solenopsins. Tetrahedron Asymmetry. 2012;23:635–42.

    Article  CAS  Google Scholar 

  • Pinto JRAS, Fox EGP, Saidemberg DM, Santos LD, Silva MAR, Costa-Manso E, Machado EA, Bueno OC, Palma MS. Proteomic view of the venom from the fire ant Solenopsis invicta Buren. J Proteome Res. 2012;11:4643–53.

    Article  Google Scholar 

  • Prahlow JA, Barnard JJ. Fatal anaphylaxis due to fire ant stings. Am J Forensic Med Pathol. 1989;19:137–42.

    Article  Google Scholar 

  • Rashid T, Chen J, McLeod P. Toxicity of newly isolated piperideine alkaloids from the red imported fire ant Solenopsis invicta Buren against the green peach aphid Myzus persicae (Sulzer). Adv Entomol. 2013;1:20–3.

    Article  Google Scholar 

  • Read GW, Lind NK, Oda CS. Histamine release by fire ant (Solenopsis) venom. Toxicon. 1978;16:361–7.

    Article  PubMed  CAS  Google Scholar 

  • Schmidt M, McConnell TJ, Hoffman DR. Production of a recombinant imported fire ant venom allergen Sol i 2 in native and immunoreactive form. J Allergy Clin Immunol. 1996;98(1):82–8.

    Article  PubMed  CAS  Google Scholar 

  • Stablein JJ, Lockey RF, Hensel AE. Death from imported fire ant stings. Immunol Allergy Pract. 1985;7:279–82.

    Google Scholar 

  • Stafford CT. Hypersensitivity to fire ant venom. Ann Allergy Asthma Immunol. 1996;77:87–95.

    Article  PubMed  CAS  Google Scholar 

  • Sukprasert S, Uawonggul N, Jamjanya T, Thammasirirak S, Daduang J, Daduang S. Characterization of the allergen Sol gem 2 from the fire ant venom Solenopsis geminata. J Venom Anim Toxins Incl Trop Dis. 2012;18(3):325–34.

    Article  CAS  Google Scholar 

  • Tschinkel WS. The fire ants. Cambridge: Belknap Press of Harvard University Press; 2006.

    Google Scholar 

  • Wetterer JK. Worldwide spread of the tropical fire ant, Solenopsis geminata (Hymenoptera: Formicidae). Myrmecol News. 2011;14:21–35.

    Google Scholar 

  • Yeh JZ, Narahashi T, Almon RR. Characterization of neuro-muscular blocking action of piperidine derivatives. J Pharmacol Exp Ther. 1975;194:373–83.

    PubMed  CAS  Google Scholar 

  • Yi GB, McClendon D, Desaiah D, Goddard J, Lister A, Moffit J, Vender Meer RK, de Shazo R, Lee KS, Rockhold RW. Alkaloid component of Solenopsis invicta (fire ant) venom inhibits brain nitric oxide synthase. Pharmacologist. 2001;43:109.

    Google Scholar 

  • Yi GB, McClendon D, Desaiah D, Goddard J, Lister A, Moffitt J, Meer RK, deShazo R, Lee KS, Rockhold RW. Fire ant venom alkaloid isosolenopsin A a potent and selective inhibitor of neuronal nitric oxide synthase. Int J Toxicol. 2003;22(2):81–6.

    Article  PubMed  CAS  Google Scholar 

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Authors and Affiliations

  1. Département d’Ecologie et Evolution, UNIL-Sorge Le Biophore, Lausanne, VD, 1015, Switzerland

    Eduardo Gonçalves Paterson Fox

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  1. Eduardo Gonçalves Paterson Fox
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Correspondence to Eduardo Gonçalves Paterson Fox .

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Editors and Affiliations

  1. Yong Loo Lin School of Medicine, National University of Singapore, Singapore, Singapore

    P. Gopalakrishnakone

  2. Inst.Biomedicina de Valencia IBV-CSIC, Valencia, Spain

    Juan J. Calvete

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Fox, E.G.P. (2014). Venom Toxins of Fire Ants. In: Gopalakrishnakone, P., Calvete, J. (eds) Venom Genomics and Proteomics. Springer, Dordrecht. https://doi.org/10.1007/978-94-007-6649-5_38-1

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  • DOI: https://doi.org/10.1007/978-94-007-6649-5_38-1

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  • Online ISBN: 978-94-007-6649-5

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