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HPTLC: Herbal Drugs and Fingerprints

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Herbal Drugs and Fingerprints

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Abstract

HPTLC is used for determining the quality and possible adulteration in herbal raw material and herbal products. HPTLC fingerprints are used by regulatory authorities to compare various samples with standards/reference standard. The recent trends of implementation of quality management policies in pharmaceuticals are to produce best quality of drugs. After using synthetic drugs for some decades with case history of curing some diseases successfully with many side-effects, there is a relook for herbal drugs and their strength for diseases incurable with synthetic drugs. The reliable chromatographic fingerprints are generated through the chromatographic separation of all active components present in the herbal drugs. HPTLC has very powerful separation ability, even for complex chemical components of herbals and products. Complex extracts can be easily separated into relatively simple subfractions, evaluated for stability as well as consistency for composition, even by different manufacturers.

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References

  1. Giri L, Andola HC, Purohit VK, Rawat MSM, Rawal RS, Bhatta ID. Chromatographic and spectral fingerprinting standardization of traditional medicines: an overview as modern tools. Res J Phytochem. 2010;4:234–41.

    Article  Google Scholar 

  2. Rajkumar T, Sinha BN. Chromatographic fingerprint ­analysis of budmunchiamines in Albizia amara by HPTLC technique. Int J Res Pharm Sci. 2010;1(3):313–6.

    CAS  Google Scholar 

  3. Wall PE. Thin layer chromatography: a modern ­practical approach, RCS chromatography monograph. Cambridge: Royal Society of Chemistry; 2005. ISBN 0-85404-535-X.

    Google Scholar 

  4. Andola HC, Rawal RS, Rawat MSM, Bhatta ID, Purohit VK. Analysis of berberine content using HPTLC fingerprinting of root and bark of three Himalayan berberis species. Asian J Biotechnol. 2010;2(4):239–45.

    Article  CAS  Google Scholar 

  5. Cimpoiu C. Qualitative and quantitative analysis by hyphenated (HP)TLC-FTIR technique. J Liq Chromatogr Relat Technol. 2005;28:1203–13.

    Article  CAS  Google Scholar 

Bibliography

  • Ahmad I, Aqil F, Owais M. Turning medicinal plants into drugs. Modern phytomedicine, vol. 384. Weinheim: Wiley; 2006. p. 67–72.

    Google Scholar 

  • Bhutani KK. Fingerprinting of Ayurvedic drugs. East Pharm. 2000;507:21–6.

    Google Scholar 

  • Bobby N, Wesely EG, Johnson M. HPTLC profile studies on the alkaloids of Albizia lebbeck. Asian Pac J Trop Biomed. 2012;2:1–3.

    Article  Google Scholar 

  • Dhandapani A, Kadarkarai M. HPTLC quantification of flavonoids, larvicidal and smoke repellent activities of Cassia occidentalis L. (Caesalpiniaceae) against malarial vectore Anopheles Stephensi Lis (Diptera: Culicidae). J Phytol. 2011;3(2):60–71.

    CAS  Google Scholar 

  • Liang YZ, Xie P, Chan K. Quality control of herbal medicines. J Chromatogr B. 2004;812:53–70.

    CAS  Google Scholar 

  • Long F. Bio-pharmaceutical characterization of herbal medicinal products. Drugs. 2001;44(4):102–8.

    Google Scholar 

  • Sagar BPS, Zafar R, Panwar R. Herbal drug standardization. Indian Pharm. 2005;4(35):19–22.

    Google Scholar 

  • Shahare MD, Mello PM. Standardization of Bacopa monnieri and its formulations with reference to Bacoside A, by high performance thin layer chromatography. Int J Pharmacogn Phytochem Res. 2010;2(4):8–12.

    Google Scholar 

  • Shanbhag DA, Khandagale NA. Application of HPTLC in the standardization of a homoeopathic mother tincture of Syzygium jambolanum. J Chem Pharm Res. 2011;3(1):395–401.

    CAS  Google Scholar 

  • Soni K, Naved T. HPTLC–its applications in herbal drug industry. Pharma Rev. 2010 (July-August);112–7.

    Google Scholar 

  • WHO. Quality control methods for medicinal plant materials. Geneva: WHO; 1998.

    Google Scholar 

  • Yadav D, Tiwari N, Gupta MM. Simultaneous quantification of diterpenoids in Premna integrifolia using a validated HPTLC method. J Sep Sci. 2011;34(3):286–91.

    Article  PubMed  CAS  Google Scholar 

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Authors and Affiliations

  1. Amity Institute of Phytochemistry & Phytomedicine, Amity University, Uttar Pradesh, Noida, UP, India

    Devi Datt Joshi

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  1. Devi Datt Joshi
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Correspondence to Devi Datt Joshi .

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© 2012 Springer India

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Joshi, D.D. (2012). HPTLC: Herbal Drugs and Fingerprints. In: Herbal Drugs and Fingerprints. Springer, India. https://doi.org/10.1007/978-81-322-0804-4_3

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  • DOI: https://doi.org/10.1007/978-81-322-0804-4_3

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  • Publisher Name: Springer, India

  • Print ISBN: 978-81-322-0803-7

  • Online ISBN: 978-81-322-0804-4

  • eBook Packages: MedicineMedicine (R0)

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